Systemic lupus erythematosus detection method

Abstract

The invention discloses a systemic lupus erythematosus detection method. The systemic lupus erythematosus detection method comprises the following steps: drawing 3ml of blood of a patient into a disposable vacuum blood collection tube without an additive; placing the disposable vacuum blood collection tube at room temperature for coagulating; sucking a leucocytes layer from the disposable vacuum blood collection tube by using a disposable suction tube; heating a suction opening of the disposable suction tube through a lighter; tightly clamping the suction opening by a pair of forceps after the suction opening is softened, so as to seal the suction opening; cutting off the head and the tail of the suction tube by a pair of scissors and putting the suction tube into a disposable plastic hose; putting the disposable plastic hose into a centrifugal machine, and centrifuging and precipitating at the speed of 2000r / min for 10 minutes; inserting a tip micro-suction tube into the disposable suction tube to suck away upper-layer blood serum; then slightly sucking the leucocytes layer to prepare 3 to 4 pieces of thin blood films; staining the thin blood films with a Wright-Giemsa staining solution and then carrying out microscope inspection. According to the systemic lupus erythematosus detection method, the leucocytes layer is sealed in the disposable suction tube and then is concentrated again, so that the positive rate is greatly increased, and the screening rate of lupus erythematosus cells and the detection accuracy are improved.

Description

technical field [0001] The invention relates to a systemic lupus erythematosus detection method, which belongs to the technical field of medical detection. Background technique [0002] The word lupus comes from Latin and appeared in Western medicine around the 19th century. But it was not until the middle of the 19th century that a doctor named Carson Love officially used the medical term "lupus erythematosus". However, the lupus he mentioned only refers to discoid lupus erythematosus, which is mainly caused by skin lesions. People with this disease have recurrent intractable and refractory skin lesions on the face or other related parts, and some also have atrophy, scars, and plain color changes on the basis of erythema, which deform the face and severely disfigure it. , It looks like it has been bitten by a wolf, so it has its name. Later, with the continuous development of medical science, people's understanding of lupus erythematosus gradually deepened, so the name s...

AI Technical Summary

Problems solved by technology

Therefore, LE (lupus erythematosus) cell examination is one of the screening indicators for the diagnosis of systemic lupus erythematosus, see figure 1 , the traditional method is to directly collect the white blood cell layer by centrifugation in the vacuum blood collection tube. Due to the large diameter of the vacuum blood collection tube, the white blood cell column layer formed is small, and the number of white blood cells absorbed is small. The positive detection rate is often very low, and there is even a possibility of missed detection.

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