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Dendrobium officinale-moss coexisting culture system

A Dendrobium officinale and culture system technology, applied in the direction of culture medium, cultivation, soilless cultivation, etc., can solve the problems affecting the survival rate and growth stability of planting, erosion and invasion of forest planting, and unstable growth environment, etc. Utilization rate, increase utilization rate, effect of widening optional area

Inactive Publication Date: 2017-03-08
SHAOXING UNIV YUANPEI COLLEGE
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

But the growth is slow, and the utilization rate of suitable forest land is low, and the yield is scarce. At the same time, due to the irregular growth of forest trees, mountain forest slopes and other terrains, the growth environment will be unstable, and the water flow will wash and invade the forest planting, affecting the survival of planting. rate and growth stability

Method used

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  • Dendrobium officinale-moss coexisting culture system
  • Dendrobium officinale-moss coexisting culture system
  • Dendrobium officinale-moss coexisting culture system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0042] Materials and Methods

[0043] 1.1 Experimental materials

[0044] Moss: There are many kinds of mosses on the trees in the nearby park and in the shade on the wall.

[0045] Dendrobium candidum: Tissue culture seedlings of a large Dendrobium candidum planting base in Zhejiang Province.

[0046] Main instruments: fluorescence microscope (Nikon), PCR (Bio-rad), gel imaging system (Bio-rad), nucleic acid and protein analyzer (Bio-rad).

[0047] experimental method

[0048] 1.2.1 Disinfection of moss

[0049] Select fresh moss, soak it in 0.1% sodium hypochlorite for 30 minutes, shake it on a shaker for 4 hours, soak it in distilled water and wash it, and dry it with sterile filter paper.

[0050] The configuration of the moss medium

[0051] There are two kinds of moss tissue culture medium, Knop medium and Benecke medium.

[0052] Moss DNA extraction and identification

[0053] Take fresh moss, add liquid nitrogen to grind, add CTAB (containing 0.1% mercaptoe...

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Abstract

The invention relates to a dendrobium officinale-moss coexisting culture system, and belongs to the technical field of horticulture and forestry. A corrosion-resistant wood base is wrapped by associated mosses or a combination of the associated mosses and tree bark, the corrosion-resistant wood base and the associated mosses or the associated mosses and the tree bark form a support, dendrobium officinale tissue culture seedlings are fixed on the support, and the support is fixed on tree-shaded forest land. The system is applied to planting of dendrobium officinale, and the system has the advantages of being capable of achieving three-dimensional planting, increasing the forest utilization area, reducing growing environment impacts and the like.

Description

technical field [0001] The invention relates to a dendrobium officinale-moss symbiosis culture system, which belongs to the technical field of horticulture and forestry. Background technique [0002] Dendrobium officinale ( Dendrobium officinale Kimura et Migo ) is a traditional Chinese medicinal material in my country, mainly distributed in Anhui, Zhejiang, Fujian and other places. It has unique effects such as benefiting the stomach and promoting body fluid, nourishing yin and clearing away heat. As early as the Qin and Han Dynasties, "Shen Nong's Materia Medica" recorded Dendrobium officinale as "sweet and flat. In the main injury, it eliminates numbness, lowers qi, nourishes five viscera and fatigue, wins weakness, and strengthens yin. Long-term use can thicken the stomach, lighten the body, Extend the year". More than 1,000 years ago, "Tao Zang" listed Dendrobium candidum as the first of the "Nine Great Immortal Grasses in China"; Li Shizhen commented in "Compendium ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/02A01G31/00
CPCA01G24/00A01G31/02Y02P60/21
Inventor 寿建昕梅雪娜孔伟晶余莎
Owner SHAOXING UNIV YUANPEI COLLEGE
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