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Method for asexual cloning and quick propagation of high-quality Shixing Dendrobium seedlings

A Shixing stone and seedling technology, which is applied in the field of plant regeneration, can solve the problems of unfavorable commercial production and uneven shape of the offspring, and achieve the effect of low cost

Active Publication Date: 2017-03-08
南京大花滩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to explore the asexual rapid propagation method of Shixing Dendrobium to solve the separation phenomenon and uneven shape of the breeding offspring, which is not conducive to the problem of commercial production

Method used

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  • Method for asexual cloning and quick propagation of high-quality Shixing Dendrobium seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Material collection: In the growing season, the young shoots of the year's best individual plants that were vigorously growing in the Shixing Dendrobium Germplasm Garden were selected as explants in the South China Botanical Garden.

[0031] 2. Select Shixing Dendrobium strains, rinse them with water, cut off the leaves, soak in 70% alcohol for 30 seconds, then sterilize with 0.1% mercuric chloride solution for 5 minutes, rinse with sterile water 4 to 5 times, and cut out 1 to 1 The 2 cm section of the stem segment is inoculated into the protocorm-like induction medium M1. After 45 days, the stem segment has axillary buds on the medium, and the incision at the base of the stem segment expands to form a granule protrusion; it is transferred to the above-mentioned medium M1, and then After 50 days of cultivation, protocorm-like is formed, and the culture temperature of the induced protocorm-like stage is 25±2°C, the illuminance is 1500Lx, and the light is 12 hours / day; ...

Embodiment 2

[0037] 1. Material collection: In the growing season, the young shoots of the year's best individual plants that were vigorously growing in the Shixing Dendrobium Germplasm Garden were selected as explants in the South China Botanical Garden.

[0038] 2. Select Shixing Dendrobium strains, rinse them with water, cut off the leaves, soak in 70% alcohol for 30 seconds, then sterilize with 0.1% mercuric chloride solution for 10 minutes, rinse with sterile water 4 to 5 times, and cut 1 to 5 The 2 cm section of the stem segment is inoculated into the protocorm-like induction medium M1. After 45 days, the stem segment has axillary buds on the medium, and the incision at the base of the stem segment expands to form a granule protrusion; it is transferred to the above-mentioned medium M1, and then After 50 days of cultivation, protocorm-like is formed, and the culture temperature of the induced protocorm-like stage is 25±2°C, the illuminance is 2000Lx, and the light is 12 hours / day; the...

Embodiment 3

[0044] 1. Material collection: In the growing season, the young shoots of the year's best individual plants that were vigorously growing in the Shixing Dendrobium Germplasm Garden were selected as explants in the South China Botanical Garden.

[0045] 2. Select Shixing Dendrobium strains, rinse them with water, cut off the leaves, soak in 70% alcohol for 30 seconds, then sterilize with 0.1% mercuric chloride solution for 7.5 minutes, rinse with sterile water 4 to 5 times, and cut out 1 to 1 The 2 cm section of the stem segment is inoculated into the protocorm-like induction medium M1. After 45 days, the stem segment has axillary buds on the medium, and the incision at the base of the stem segment expands to form a granule protrusion; it is transferred to the above-mentioned medium M1, and then After 50 days of cultivation, protocorm-like is formed, and the culture temperature of the induced protocorm-like stage is 25±2°C, the illuminance is 1800Lx, and the light is 12 hours / day...

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Abstract

The invention discloses a method for asexual cloning and quick propagation of Shixing Dendrobium seedlings, and the method comprises the following main steps: preprocessing, inducing culture, protocrom-like body formation, protocrom-like body proliferation, differentiation culture, rooting and seedling strengthening and transplanting, and in the process, a protocrom-like body inducing culture medium is M1, a proliferation culture medium is M2, a differentiation culture medium is M3 and a rooting and seedling strengthening medium is M4. According to the method, a Shixing Dendrobium high-quality individual plant is selected, a plant tissue culture technique is used for rapid and mass propagation of high quality seedlings, and the large-scale and commercial production of the Shixing Dendrobium seedlings are facilitated. Only simple plant tissue culture equipment is used in the method for successful asexual cloning and quick and mass propagation of Shixing Dendrobium, and the method has the advantages of low cost and high efficiency.

Description

technical field [0001] The invention relates to the field of plant regeneration through tissue culture technology, in particular to a method for rapid propagation of asexual clones of high-quality seedlings of Dendrobium shixing and the culture medium used. Background technique [0002] Dendrobium shixingense, Latin name Dendrobium shixingense, was discovered in Shixing County, northern Guangdong in 2010. Now it is only found in Guangdong and a few areas on the border with Jiangxi. It is suitable for growing at an altitude of about 400-600 meters. It has important medicinal uses , ornamental, scientific research, protection and other values. Compared with Dendrobium candidum with good market value at present, the leaves and stems of Shixing Dendrobium are reddish-brown, more ornamental, purple-red in color, more attractive in color, stronger in chewing stickiness, and sweeter in taste. Its germination rate is high, stress resistance, disease and insect resistance are strong...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 史月龙吴坤林曾宋君吴玲祥陈德伟赵宏群马翠萍芮建汤静
Owner 南京大花滩生物科技有限公司
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