52results about How to "Realize commercial production" patented technology

The invention belongs to the technical fields of biotechnology, biological medical materials and tissue engineering, in particular to injected chemical crosslinking protein / polypeptide hydrogel and a preparation method thereof. The injected chemical crosslinking protein / polypeptide hydrogel consists of natural or synthesized protein / polypeptide with a crosslinking phenolic hydroxyl group, horse radish peroxidase and hydrogen peroxide which quickly form the chemical crosslinking hydrogel under physiological condition. The protein / polypeptide hydrogel has good biocompatibility and easily regulated mechanical strength, and is degradable, and the degraded product is amino acids capable of being absorbed by a human body. In addition, the framework of the protein / polypeptide hydrogel very approaches a protein three-dimensional network which supports the cell growth in extracellular matrix, and the invention can be widely applied to multiple biological and medical fields such as drug controlled-release carriers, cell culture supports and the like.

The invention discloses a liquid fermentation type fertilizer for pear trees and a preparation method of the fertilizer. The fertilizer is prepared from raw materials in parts by weight as follows: industrial starch, soybean meal, a compound microbial agent, bran, earthworm soil, withered leaves, humic acid, saturated lime water, monopotassium phosphate, ammonium bicarbonate, boric acid, quartz sand, a coagulant and a proper amount of water. With the adoption of the liquid fermentation manner, the fertilizer is fermented more completely, various ingredients beneficial to crops are dissolved in water and then are coagulated through the special coagulant and spin-dried. The preparation method is used for processing of the fertilizer.

The invention relates to preparation of chromatogram, which concretely is using free radical polymerization method to prepare linking polysaccharide chiral stationary phase ,including the following steps of reaction: 1) prepare derivative of polysaccharide containing methacrylate; 2)prepare derivative of silica gel 3) free radical polymerization reaction . Its advantages are high efficiency of chiral stationary phase prepared, wide field of use, suitable for each kind of mibile phase, sample and rapid preparation.

The invention discloses a modular cross-wall structure for a civil work, which is formed by splicing a plurality of retaining walls laterally. Each retaining wall is mainly formed by a base, a plurality of regulating building blocks and a cover board; each base is vertically provided with integrally formed foundation building blocks; longitudinal positioning grooves on the lower surfaces of the regulating building blocks on each upper layer are clamped on longitudinal positioning convex strips on the upper surfaces of the regulating building blocks on the adjacent lower layer; the longitudinal positioning grooves on the lower surfaces of the regulating building blocks on the lowermost layer are clamped on longitudinal positioning convex strips on the upper surfaces of the foundation building blocks; longitudinal positioning grooves on the lower surfaces of the cover boards are clamped on the longitudinal positioning convex strips on the upper surfaces of the regulating building blocks on the uppermost layer; and one end of each base is provided with a dovetail-shaped lock connector and the other end of the base is provided with a locking groove matched with the lock connector so as to implement locking of the left and right bases. The modular cross-wall structure adopts the modular design, is convenient and rapid to construct and has no technical requirements. Anyone can construct the modular cross-wall structure after simple training. Each component can be uniformly made well in a factory and then transported to the field to be installed; commercial production can be implemented.

The invention relates to preparation and application of a fluorescent carbon dot nanoprobe for detecting methylene blue and living cell imaging. Under the excitation wavelength of 320nm, the maximum emission wavelength of carbon dots (CDs) appears at 440nm and strong blue fluorescence is emitted. Methylene blue can be used for quenching the fluorescence of the carbon dots, and in a cell culture dish, a living cell incubates for 24 hours with the methylene blue, and the cell is observed by using a laser confocal microscopy system and continuously emits blue fluorescence. According to the preparation and the application, the carbon dots carbonized by semen litchi can be used for detecting the methylene blue by quenching a fluorescent signal. As an effective means for detecting the methylene blue, the method shows a remarkable advantage. The detection limit is as low as 0.05micro mol / L and the linear response range is 0.8-10micro mol / L [R<2>=0.9930]. As being a fluorescent probe, a sensing system has the optical characteristics of simplicity, low cost, greenness, high selectivity, rapidness and sensitivity and is successfully applied to methylene blue detecting and living cell imaging analysis. The sensing method has a broad application prospect in the field of environmental analysis and the cell imaging analysis.

The invention provides a Bacillus strain with antagonistic action on root rot. The Bacillus strain has a scientific name of Bacillus subtilis FJAT-20265 and is preserved in China General Microbiological Culture Collection Center under the accession number of CGMCC No. 14430. The Bacillus subtilis FJAT-20265 screened in the invention has strong inhibitory effect on main pathogenic bacteria of root rot, i.e., Fusarium oxysporum, Fusarium solani and Fusarium moniliforme, and can be specially used for controlling root rot.

The invention discloses a method for mechanical, chemical and biological combined solidification of copper tailings and preparation of engineering filler, and provides a packaged technology for material processing, roadbed construction and slope reinforcement. The mass percent is controlled for preparation of a tailing and gravel mixture according to the principle that the content of each heavy metal element of the tailing and gravel mixture meets the national standard and the principle that the content of the particle group with the particle size larger than 0.075 mm is higher than 50%, a proper amount of Portland cement is added, mixed and spread, then the mixture is controlled to be layered and compacted under the optimum water content ranging from 13% to 17%, and the mixture can be applied to engineering after the seepage concentration of each heavy metal element is tested to be lower than the national standard according to a leaching experiment. Ecological slope reinforcement is realized through planting of copper enriching plants during construction, so that migration of the heavy metal elements is further controlled. According to the method, mechanical, chemical and biological solidification technologies are utilized comprehensively, and a three-dimensional closed system of tailing and gravel mixed soil is constructed. The requirement for reduction of solid waste in thewhole process of copper smelting is realized, occupation of cultivated land due to the requirement for a large amount of engineering earthwork can also be avoided, and thus the harm is turned into thebenefit.

The invention relates to preparation of chromatogram, which concretely is using free radical polymerization method to prepare linking polysaccharide chiral stationary phase ,including the following steps of reaction: 1) prepare derivative of polysaccharide containing methacrylate; 2)prepare derivative of silica gel 3) free radical polymerization reaction . Its advantages are high efficiency of chiral stationary phase prepared, wide field of use, suitable for each kind of mibile phase, sample and rapid preparation.

A quadruplex hybridization method for culturing new variety of meat ox includes such steps as the ternary hybridization between German ox, Nanyang ox and Hongangesi ox as the secondary male parent, choosing male group, the ternary hybridization between Ximentaer ox, Nanyang ox and Hongangesi ox as the secondary male parent, choosing female group, and the hybridization between said made group and female group, and preferable culture.

The invention belongs to the technical field of plant tissue culture, and particularly relates to a clonal propagation method of paphiopedilum armeniacum. The clonal propagation method of the paphiopedilum armeniacum comprises the following steps: (1) obtaining and treating of explants; (2) collection of the explants; (3) surface disinfection of the explants; (4) induction culture; (5) cluster budinduction; (6) multiplication culture; (7) seedling strengthening and rooting culture; (8) test-tube plantlet transplanting. Compared with the prior art, the clonal propagation method disclosed by the invention has the advantages that the explants are simple in material taking, are easy to obtain, are large in amount and cannot damage a stock plant, a tissue culture process is simple and feasible, the seedling formation period is short, the product consistency is high, the whole quality standard is controllable, and the like; the clonal propagation method is a simple, fast and effective propagation method for realizing commercialized production of the paphiopedilum armeniacum.

The invention discloses a method for culturing and rapidly breeding high-quality paphiopedilum seedling tissues by leafage buds. According to the method, lateral buds of paphiopedilum serve as explants, the leafage buds are induced, proliferated and disintegrated into normal adventitious buds by the aid of unique cutting of the explants and a culture medium, rooting and strong seedling culture isimplemented to obtain a lot of uniform tissue culture seedlings. According to the method for culturing and rapidly breeding the high-quality paphiopedilum seedling tissues by the leafage buds, the leafage buds can be successfully induced, proliferated and reset into the normal adventitious buds, each cluster of leafage buds acquire adventitious buds of 89-122 pieces within half year, rooting and strong seedling culture of 6-8 months of the acquired leafage buds is implemented and can be transplanted, and large-scale and commercialization production of the paphiopedilum tissue culture seedlingsis achieved. The culture method and components of the used culture medium are unique, simple and practical, a culture system is stable, a technique is operable, high in application value, and an effective path is provided for improvement of breeding efficiency and market requirement meeting of the paphiopedilum.