52results about How to "Realize commercial production" patented technology

The invention belongs to the technical fields of biotechnology, biological medical materials and tissue engineering, in particular to injected chemical crosslinking protein / polypeptide hydrogel and a preparation method thereof. The injected chemical crosslinking protein / polypeptide hydrogel consists of natural or synthesized protein / polypeptide with a crosslinking phenolic hydroxyl group, horse radish peroxidase and hydrogen peroxide which quickly form the chemical crosslinking hydrogel under physiological condition. The protein / polypeptide hydrogel has good biocompatibility and easily regulated mechanical strength, and is degradable, and the degraded product is amino acids capable of being absorbed by a human body. In addition, the framework of the protein / polypeptide hydrogel very approaches a protein three-dimensional network which supports the cell growth in extracellular matrix, and the invention can be widely applied to multiple biological and medical fields such as drug controlled-release carriers, cell culture supports and the like.

The invention relates to a sulfydryl cellulose porous material as well as a preparation method and application thereof. The sulfydryl cellulose porous material contains the raw material components of cellulose suspension liquid and a sulfydryl silane coupling agent in a mass ratio of (1 to 0.3)-(1 to 4). The preparation method comprises the following steps: adding the sulfydryl silane coupling agent into the cellulose suspension liquid, adjusting the pH value, and stirring at the room temperature, so as to obtain cellulose-silane suspension liquid; and freezing the cellulose-silane suspension liquid in liquid nitrogen so as to obtain cellulose-silane icy gel, and carrying out freeze drying, baking and curing, so as to obtain the sulfydryl cellulose porous material. Compared with the existing commercial enrichment adsorbent, namely sulfydryl cotton, in the prior art, the sulfydryl cellulose porous material has excellent performance of uniform porous mesh structure, relatively large specific surface area, relatively high sulfydryl content and good mechanical property, reaction raw materials are environment-friendly, reaction conditions are mild, and the process is simple, efficient and controllable. The sulfydryl cellulose porous material has wide application prospects in the fields of heavy metal adsorption, enrichment and detection, catalyst carriers, chromatographic separation and analysis and the like.

The invention discloses a carbon-based solid acid catalyst and a preparation method thereof. The prepared catalyst adopts activated carbon as carrier and benzenesulfonic acid as acid site which are combined through firm carbon-carbon covalent bond. The preparation method comprises the following steps: first adopting aminobenzene sulfonic acid to perform diazotization process, obtaining diazosalt of sulfanilic acid, then using hypophosphorous acid as reductant to react with diazosalt of sulfanilic acid on activated carbon, and finally filtrating, washing and drying the products after finishing the reaction to obtain the carbon-based solid acid catalyst. The carbon-based solid acid catalyst has the advantage of stable structure, strong acidity, low cost, good catalytic effect and environmental-friendliness and can be reproducible and reused.

A method for synthesis of linking polysaccharide chiral stationary phase, includes the following steps: 1) in the reactor with organic solvent, polysaccharide and derivative solvent of polysaccharide react with including functional group which can react with polysaccharide hydrocarbon radical, then though centrifugal separation the solid substance is collected; 2) coat the prepared polysaccharide derivative containing trihydroxyme or on the surface of silica gel matrixof chromatogram filler, then they are mixed with toluene and pyridine in reactor , cooled after reaction, then use tetrahydrofuran and carbinol separately to wash the creature, which are dried in vacuum, acquiring the linking polysaccharide chiral stationary phase. Its advantages are: wide field of use, simple and rapid preparation.

The invention relates to a method for manufacturing chopped carbon fiber reinforced concrete, particularly comprising the following steps: dispersing chopped carbon fiber in aqueous solution of water-soluble cellulose ether; adopting a papermaking method to filter the carbon fiber from carbon fiber suspension by a filter screen or a mop; taking down the dried carbon fiber from the filter screen or the mop; cutting the take-down carbon fiber felt into small blocks with side length being 4-8cm; dry mixing the small blocks with the concrete raw material for 1-4min, then adding water to mix for 1-4min, and finally casting and tightly molding the mixture to obtain the chopped carbon fiber reinforced concrete, wherein the dosage of carbon fiber accounts for 0.2-2.5% of the total volume of the chopped carbon fiber reinforced concrete. The invention is beneficial to blending and dispersing the carbon fiber and the concrete, improving the tightness of the concrete, lowering production cost and mixing a large amount of the chopped carbon fiber reinforced concrete with stable and uniform performance on-site.

The invention discloses a liquid fermentation type fertilizer for pear trees and a preparation method of the fertilizer. The fertilizer is prepared from raw materials in parts by weight as follows: industrial starch, soybean meal, a compound microbial agent, bran, earthworm soil, withered leaves, humic acid, saturated lime water, monopotassium phosphate, ammonium bicarbonate, boric acid, quartz sand, a coagulant and a proper amount of water. With the adoption of the liquid fermentation manner, the fertilizer is fermented more completely, various ingredients beneficial to crops are dissolved in water and then are coagulated through the special coagulant and spin-dried. The preparation method is used for processing of the fertilizer.

The invention relates to preparation of chromatogram, which concretely is using free radical polymerization method to prepare linking polysaccharide chiral stationary phase ,including the following steps of reaction: 1) prepare derivative of polysaccharide containing methacrylate; 2)prepare derivative of silica gel 3) free radical polymerization reaction . Its advantages are high efficiency of chiral stationary phase prepared, wide field of use, suitable for each kind of mibile phase, sample and rapid preparation.

The invention discloses a chemically-bonded cellulose-type chiral stationary phase and a preparation method thereof. The preparation method comprises the following steps: (1) adding cellulose derivatives, which are obtained by reactions between cellulose and derivatives thereof, into a reaction container containing an organic solvent, washing and drying the cellulose derivatives, and saving cellulose derivatives for later use; (2) painting the prepared cellulose derivatives on the surface of aminopropyl silica gel, evaporating the solvent, adding a new solvent and a joint arm reagent to carry out reactions, cooling, then washing the reaction products by methanol and tetrahydrofuran, and drying the reaction products in vacuum so as to obtain the chemically-bonded cellulose-type chiral stationary phase. The prepared chiral stationary phase has the advantages of wide application range, good chromatographic performance, and high chemical stability, and can be applied to commercial production.

The invention provides bacillus for antagonizing fusarium wilt and promoting growth and an application of the bacillus. The bacterial strain is bacillus simplex FJAT-47158, and the chemical name of the bacterial strain is Bacillus simplex FJAT-47158. The bacterial strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the bacterial strainis CGMCC NO. 15946. The bacillus disclosed by the invention not only can inhibit phytopathogens such as fusarium oxysporum, xanthomonas carpetalis and the like, but also can improve the activity of plant seeds and promote the growth of the seeds, can be used for preventing and treating fusarium wilt, and is an environment-friendly, economical and effective biocontrol bacterium for preventing andtreating diseases.

The invention discloses a method for preparing a mitochondria targeted spinning scavenger MitoPBNs (spinning probe). The structural formula of the MitoPBN is shown in a formula (I). A synthesis flow is shown in the formula (II), and comprises the following steps of: performing elimination reaction on 4-hydroxybenzaldehyde and dibromo-straight-chain paraffin under alkali condition to prepare a compound 3; reacting the compound 3 with triphenylphosphine to prepare the compound 4; in ethanol, mixing the compound 4, 2-nitro-2-methylpropane, a 4A molecular sieve and zinc powder in a solvent, adding dropwise a glacial acetic acid, stirring and reacting the mixed solution at room temperature, placing the mixed solution in a refrigerator for refrigeration, and performing separation and purification to prepare the spinning probe MitoPBN; and performing liposome packing treatment. The method has the characteristics of relatively fewer synthesis steps, low raw material cost, readily available raw materials and relatively higher product purity. The probe for scavenging free radical signals can enter cell mitochondria.

The invention provides Bacillus for antagonizing blight and application thereof, wherein the strain has a scientific name of Bacillus siamensis FJAT-47582. The Bacillus siamensis FJAT-47582 is preserved in the China General Microbiological Culture Collection Center (CGMCC), with the preservation number of CGMCC NO. 15947. The Bacillus of the invention can not only inhibit fusarium oxysporum, xanthomonas axonopodis and other plant pathogenic bacteria, but also improve the vitality of plant seeds. The bacillus can be used for preventing and treating blight, and is a biocontrol bacterium being environment-friendly, economic and effective in terms of disease prevention and control.

The invention provides bacillus for antagonizing phytopathogens and promoting rooting and an application of the bacillus. The bacterial strain is bacillus velezensis FJAT-47164, and the chemical nameof the bacterial strain is Bacillus velezensis FJAT-47164. The bacterial strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the bacterial strain is CGMCC NO. 15948. The bacillus disclosed by the invention not only can inhibit phytopathogens, but also can promote plant rooting, can be used for early prevention of fusarium wilt, and isan environment-friendly, economical and effective biocontrol bacterium for preventing and treating diseases.

The invention provides a special culture medium for a blueberry and a production process thereof, belonging to the technical field of crop cultivation. According to the invention, vinegar residues are used as a main material, 1 to 5% of urea is added to adjust a carbon-nitrogen ratio to 25: 1 to 20: 1; 0.1 to 0.5% of a composite microbial strain is added; after completion of microbial fermentation and decomposition, the pH value of a material is 6.7 to 6.9 and is adjusted to 4.5 to 5.0 by using tail vinegar; and a finished product procedure is carried out so as to prepare the special culture medium for the blueberry. The special culture medium for the blueberry is an acidic granular medium containing no less than 60% of organic matters, 4.0 to 4.5% of total nutrients, 30 to 35% of moisture content, a soluble ion concentration EC value of 1 to 2 mS / cm, 22 to 25% (V) of ventilation porosity and 44 to 46% (V) of water-retention porosity. Test results show that the special culture medium for the blueberry is used for blueberry cultivation and has better effect than a turf medium. According to the invention, the waste is turned into the valuable; exploitation of the resource of turf is reduced; and the environment is protected.

The invention discloses a method for asexual cloning and quick propagation of Shixing Dendrobium seedlings, and the method comprises the following main steps: preprocessing, inducing culture, protocrom-like body formation, protocrom-like body proliferation, differentiation culture, rooting and seedling strengthening and transplanting, and in the process, a protocrom-like body inducing culture medium is M1, a proliferation culture medium is M2, a differentiation culture medium is M3 and a rooting and seedling strengthening medium is M4. According to the method, a Shixing Dendrobium high-quality individual plant is selected, a plant tissue culture technique is used for rapid and mass propagation of high quality seedlings, and the large-scale and commercial production of the Shixing Dendrobium seedlings are facilitated. Only simple plant tissue culture equipment is used in the method for successful asexual cloning and quick and mass propagation of Shixing Dendrobium, and the method has the advantages of low cost and high efficiency.

The invention discloses a modular cross-wall structure for a civil work, which is formed by splicing a plurality of retaining walls laterally. Each retaining wall is mainly formed by a base, a plurality of regulating building blocks and a cover board; each base is vertically provided with integrally formed foundation building blocks; longitudinal positioning grooves on the lower surfaces of the regulating building blocks on each upper layer are clamped on longitudinal positioning convex strips on the upper surfaces of the regulating building blocks on the adjacent lower layer; the longitudinal positioning grooves on the lower surfaces of the regulating building blocks on the lowermost layer are clamped on longitudinal positioning convex strips on the upper surfaces of the foundation building blocks; longitudinal positioning grooves on the lower surfaces of the cover boards are clamped on the longitudinal positioning convex strips on the upper surfaces of the regulating building blocks on the uppermost layer; and one end of each base is provided with a dovetail-shaped lock connector and the other end of the base is provided with a locking groove matched with the lock connector so as to implement locking of the left and right bases. The modular cross-wall structure adopts the modular design, is convenient and rapid to construct and has no technical requirements. Anyone can construct the modular cross-wall structure after simple training. Each component can be uniformly made well in a factory and then transported to the field to be installed; commercial production can be implemented.

The invention relates to preparation and application of a fluorescent carbon dot nanoprobe for detecting methylene blue and living cell imaging. Under the excitation wavelength of 320nm, the maximum emission wavelength of carbon dots (CDs) appears at 440nm and strong blue fluorescence is emitted. Methylene blue can be used for quenching the fluorescence of the carbon dots, and in a cell culture dish, a living cell incubates for 24 hours with the methylene blue, and the cell is observed by using a laser confocal microscopy system and continuously emits blue fluorescence. According to the preparation and the application, the carbon dots carbonized by semen litchi can be used for detecting the methylene blue by quenching a fluorescent signal. As an effective means for detecting the methylene blue, the method shows a remarkable advantage. The detection limit is as low as 0.05micro mol / L and the linear response range is 0.8-10micro mol / L [R<2>=0.9930]. As being a fluorescent probe, a sensing system has the optical characteristics of simplicity, low cost, greenness, high selectivity, rapidness and sensitivity and is successfully applied to methylene blue detecting and living cell imaging analysis. The sensing method has a broad application prospect in the field of environmental analysis and the cell imaging analysis.

The invention provides bacillus for biological prevention and control of root rot and bacterial wilt. The strain is bacillus subtilis FJAT-19708, has a scientific name of Bacillus subtilis FJAT-19708and is preserved in China General Microbiological Culture Collection Center (CGMCC), with the preservation number of CGMCC No. 14431. The bacillus FJAT-19708 screened by the invention has a very strong inhibition effect on main pathogenic bacteria including fusarium oxysporum and fusarium solani of the root rot, and also has a very strong antagonistic effect on ralstonia solanacearum; the bacilluscan be used for preventing and controlling the root rot and also can be used for preventing and controlling the bacterial wilt.

The invention provides a Bacillus strain with antagonistic action on root rot. The Bacillus strain has a scientific name of Bacillus subtilis FJAT-20265 and is preserved in China General Microbiological Culture Collection Center under the accession number of CGMCC No. 14430. The Bacillus subtilis FJAT-20265 screened in the invention has strong inhibitory effect on main pathogenic bacteria of root rot, i.e., Fusarium oxysporum, Fusarium solani and Fusarium moniliforme, and can be specially used for controlling root rot.

The invention discloses a method for mechanical, chemical and biological combined solidification of copper tailings and preparation of engineering filler, and provides a packaged technology for material processing, roadbed construction and slope reinforcement. The mass percent is controlled for preparation of a tailing and gravel mixture according to the principle that the content of each heavy metal element of the tailing and gravel mixture meets the national standard and the principle that the content of the particle group with the particle size larger than 0.075 mm is higher than 50%, a proper amount of Portland cement is added, mixed and spread, then the mixture is controlled to be layered and compacted under the optimum water content ranging from 13% to 17%, and the mixture can be applied to engineering after the seepage concentration of each heavy metal element is tested to be lower than the national standard according to a leaching experiment. Ecological slope reinforcement is realized through planting of copper enriching plants during construction, so that migration of the heavy metal elements is further controlled. According to the method, mechanical, chemical and biological solidification technologies are utilized comprehensively, and a three-dimensional closed system of tailing and gravel mixed soil is constructed. The requirement for reduction of solid waste in thewhole process of copper smelting is realized, occupation of cultivated land due to the requirement for a large amount of engineering earthwork can also be avoided, and thus the harm is turned into thebenefit.

The invention discloses wrappers for quick-frozen roasted steamed stuffed buns and an application of the wrappers. The wrappers comprise 50-400 parts of potato whole powder, 600-1200 parts of wheat flour, 300-800 parts of water, 1-5 parts of trehalose, 1-6 parts of high-acyl gellan gum, 10-30 parts of table salt and 10-50 parts of modified starch P1800. The lowest bleeding rate of the wrappers ofthe quick-frozen roasted steamed stuffed buns, prepared by the formula, can reach 44.25%; when the wrappers coating fillings are applied to the roasted steamed stuffed buns, and after the reheated roasted steamed stuffed buns are subjected to sense organ assessment, the highest mark can reach 90; besides, the phenomena that fillings and cooking liquor permeate and wrappers crack do not appear; andthe quality of the quick-frozen roasted steamed stuffed buns is improved, and the commodity production of the roasted steamed stuffed buns is realized.

The invention discloses a double-unit UV photocatalytic oxidation reaction device, and belongs to the technical field of high-performance novel composite materials. The reaction device mainly comprises the following parts: (1) a three-dimensional porous foamed ceramic novel composite immobilized photocatalyst is subjected to double-sided combination, so that the specific surface area and the active reaction of the photocatalyst are increased; (2) a UVA ultraviolet lamp group is used for promoting the photocatalytic oxidation reaction rate; (3) a metal outer frame is convenient to disassemble and assemble and durable in use, and can form a multi-unit reaction system; (4) reaction devices of different specifications can be manufactured according to design requirements; (5) the metal frame can be made of stainless steel aluminum profiles and light steel materials; and (6) the reaction device can be used for an indoor environment purification device and a reaction system of industrial VOCswaste gas emission purification equipment. The double-unit UV photocatalytic oxidation reaction device subverts the conventional metal mesh photocatalyst reactor, can realize multi-unit combination,and is used for industrial VOCs waste gas emission purification treatment equipment.

The invention belongs to the technical field of plant tissue culture, and particularly relates to a clonal propagation method of paphiopedilum armeniacum. The clonal propagation method of the paphiopedilum armeniacum comprises the following steps: (1) obtaining and treating of explants; (2) collection of the explants; (3) surface disinfection of the explants; (4) induction culture; (5) cluster budinduction; (6) multiplication culture; (7) seedling strengthening and rooting culture; (8) test-tube plantlet transplanting. Compared with the prior art, the clonal propagation method disclosed by the invention has the advantages that the explants are simple in material taking, are easy to obtain, are large in amount and cannot damage a stock plant, a tissue culture process is simple and feasible, the seedling formation period is short, the product consistency is high, the whole quality standard is controllable, and the like; the clonal propagation method is a simple, fast and effective propagation method for realizing commercialized production of the paphiopedilum armeniacum.

The invention discloses a movable solar stove support structure, comprising a strove surface, a chassis and a stove ring, characterized in that the strove surface is provided with a support base, an adjustable pole base and a stove frame, the support base is provided with a coupling head, the chassis is provided with a support frame, a cover tube base and a beam, the chassis is a triangle, the top ends of the triangle is provided with an universal wheel, the two ends of the bottom is provided with a directional wheel, the stove ring is provided with a coupling head, a prolonged pole horizontally fixed. The invention makes the stove surface absorb the solar lights at a maximum extent, to make the stove follow every direction of the sun, and the absorbed energy is reflected to the location of the stove ring via the stove surface, to maintain the stove ring always locate at the focus point of the reflected rays and in a horizontal condition. The invention could sufficiently and effectively use the solar energy, to improve the heat efficiency, the operation is easy, the structure is simple, the produce cost is low and it is easy to realize commercial mass production.

The invention relates to a methanol gasoline with high stability, which is prepared from the following raw materials in volume: 70-88 percent of gasoline, 5-15 percent of methanol, 2-5 percent of diethylamine, 2-5 percent of toluene, 1-5 percent of tween 80, 2-5 percent of dodecyl sodium sulfate; the methanol gasoline is prepared by the following steps: adding the tween 80 and the dodecyl sodium sulfate in the gasoline and evenly mixing to obtain mixed solution for standby; and evenly mixing the methanol, the diethylamine and the toluene in a high-frequency electromagnetic processor for magnetic processing and immediately adding the processed solution in the mixed solution, evenly mixing to obtain the methanol gasoline. The methanol gasoline with high stability can ensure that the methanoland the gasoline are organically fused, has good stability, does not layer after long-term storage and can realize the commercialized production of the methanol gasoline.

The environment friendly digital and analog pulse width modulation (PWM) vehicle horn is one electronic horn as one vehicle part. The electronic horn includes two vibrators, PWM modulator, PWM driving stage, class-D amplifier and loudspeaker connected successively. It has two vibrators and thus can generate two tones, so that it may be used to replace available two vehicle horns for lowered making cost. It has digital and analog pulse width modulation technology adopted, so that it has high electric-to-sound conversion efficiency, relatively lower sound pressure level, and less noise interference and environmental pollution. In addition, it has also the advantages of stable performance, no need of regulation and compact structure, and may be used in automatic, motorcycle, electrically driven vehicle, ship, etc.

The invention discloses a cyperus esculentus quick-frozen baked steamed stuffed bun wrapper and application thereof. The cyperus esculentus quick-frozen baked steamed stuffed bun wrapper is prepared from 10 to 200 parts of cyperus esculentus powder, 800 to 1200 parts of wheat powder, 300 to 800 parts of water, 1 to 5 parts of mycose, 1 to 6 parts of gella gum, 10 to 30 parts of table salt and 10 to 50 parts of denatured starch P1800. The minimal water separating rate of the cyperus esculentus quick-frozen baked steamed stuffed bun wrapper prepared from the formula can reach to 41.26 percent; and when the wrapped fillers are applied to the baked steamed stuffed bun, the reheated baked steamed stuffed bun is subjected to sensory evaluation, the highest grade can reach to 91, the fillings andsoup are not permeated, the wrapper is not cracked, the quality of the quick-frozen baked steamed stuffed bun wrapper is improved, and commercialized production of the baked steamed stuffed bun is realized.