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Bacillus for antagonizing phytopathogens and promoting rooting and application of bacillus

A technology for plant pathogenic bacteria and bacillus, which is applied in the field of microorganisms and can solve the problems that plant fusarium wilt does not have a good control effect and the like

Inactive Publication Date: 2020-04-10
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these bacteria do not have a good control effect on plant wilt

Method used

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  • Bacillus for antagonizing phytopathogens and promoting rooting and application of bacillus
  • Bacillus for antagonizing phytopathogens and promoting rooting and application of bacillus
  • Bacillus for antagonizing phytopathogens and promoting rooting and application of bacillus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Screening of antagonistic strains

[0029] 1. Soil sample collection and Bacillus isolation and purification

[0030] Suspension liquid was made from yak dung in Namtso National Park in Tibet, then diluted with sterile water, and coated on LB plates (LB: tryptone 10g L -1 , sodium chloride 10g·L -1 , yeast powder 5g·L -1 , agar powder 1.5%, pH 7.0-7.2); cultivated at 30°C for 48 hours, picked a single colony, stored in glycerol, and used it as the strain to be tested. 58 strains were isolated and purified by dilution plating method as the strains to be tested.

[0031] 2. Screening of fusarium wilt biocontrol strains

[0032] (1) Preliminary screening by confrontation method

[0033] The tested strains were activated on LB medium plates and incubated at 30°C for 48 hours.

[0034] Fusarium oxysporum FJAT-30512 (Chen Qianqian, Liu Bo, Liu Guohong, Che Jianmei, Gong Haiyan. Study on the Diversity of Geobacillus in the Rhizosphere of Huazhonglou[J]. Tropica...

Embodiment 2

[0058] Identification of embodiment 2 antagonistic strains

[0059] 1. Morphological characteristics

[0060] The FJAT-47164 strain was activated on the LB plate, and the colony shape, size, color, transparency, protrusion and edge of the strain were observed after 48 hours.

[0061] The colony characteristics of strain FJAT-47164 are as follows figure 2 As shown, light yellow, round raised, opaque, neat edges, small colonies with smooth and moist surface.

[0062] 2.16S rRNA gene sequence analysis

[0063] Genomic DNA of strain FJAT-47164 was extracted by Tris-saturated phenol method, PCR amplification was carried out using 16S rRNA gene general primers 27F and 1492R, and the PCR reaction procedure was referred to the literature of Zheng Xuefang et al (Zheng Xuefang, Liu Bo, Zhu Yujing, et al. Screening and identification of biocontrol Bacillus [J]. Chinese Journal of Biological Control, 2016, 32(5): 657-665.), the PCR product was sent to Shanghai Boshang for sequencing, ...

Embodiment 3

[0065] The inhibitory effect of embodiment 3 bacillus to other bacteria

[0066] (1) Preparation of Bacillus FJAT-47164 antibacterial solution

[0067] Inoculate the Bacillus sp. FJAT-47164 strain on the LB plate, and then pick a single bacterial colony and transfer it to 10mL LB liquid medium, 170r min -1 Cultured at 30°C for 48 hours, counted by hemocytometer, the colony density of FJAT-47164 reached 2.232×10 8 cfu mL -1 . Centrifuge the FJAT-47164 fermentation broth at 4000 rpm for 8 minutes, discard the bacteria, and take the supernatant to obtain the antibacterial solution.

[0068] (2) inhibitory effect of antibacterial solution on Xanthomonas rugosa

[0069] 1 mL of Xanthomonas axonopodis (Xanthomonas axonopodis) FJAT-10151 (preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, preservation number CGMCC NO.10271) bacterial suspension (colony density 4.224 × 10 8 cfu mL -1 ) mixed with 200mL 50°C NA semi-soli...

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Abstract

The invention provides bacillus for antagonizing phytopathogens and promoting rooting and an application of the bacillus. The bacterial strain is bacillus velezensis FJAT-47164, and the chemical nameof the bacterial strain is Bacillus velezensis FJAT-47164. The bacterial strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the bacterial strain is CGMCC NO. 15948. The bacillus disclosed by the invention not only can inhibit phytopathogens, but also can promote plant rooting, can be used for early prevention of fusarium wilt, and isan environment-friendly, economical and effective biocontrol bacterium for preventing and treating diseases.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Bacillus strain which antagonizes plant pathogenic bacteria and promotes rooting. Background technique [0002] Fusarium wilt is a systemic infection disease, which is a common soil-borne disease of plants such as lyme, soybean, wheat, tomato, cotton, strawberry, etc. It is an increasingly prominent problem in the United States, Canada, Australia, and China. Due to Fusarium wilt, the leaves become smaller, lack luster, the branches are dwarfed, the fruits are thin, or the leaves turn yellow and wilt. In severe cases, the plants gradually die. The main pathogenic species of fusarium wilt are Fusarium oxysporum and Fusarium solani. The control methods include chemical reagents, fungicides, biological control, phytotherapeutic drugs, optimized fertilization, etc., and the use of biocontrol bacteria to control wilt Disease prevention is an important measure of c...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P21/00C12R1/07
CPCC12N1/20A01N63/00C12R2001/07C12N1/205
Inventor 陈峥朱育菁郑梅霞刘波许炼潘志针肖荣凤邓文琼李慧敏
Owner 福建省农业科学院农业生物资源研究所
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