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Bacillus for antagonizing blight and application thereof

A bacillus and fusarium wilt technology, applied in the field of microorganisms, can solve the problems of plant fusarium wilt not having a good control effect and the like, and achieve the effect of improving seed vigor

Inactive Publication Date: 2020-04-07
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these bacteria do not have a good control effect on plant wilt

Method used

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  • Bacillus for antagonizing blight and application thereof
  • Bacillus for antagonizing blight and application thereof
  • Bacillus for antagonizing blight and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening of antagonistic strains

[0026] 1. Soil sample collection and Bacillus isolation and purification

[0027] The rhizosphere soil under grasses in Dieshuihe Scenic Area, Tengchong, Yunnan Province was made into soil suspension, which was then properly diluted with sterile water and coated on LB plates (LB: tryptone 10g L -1 , sodium chloride 10g·L -1 , yeast powder 5g·L -1 , agar powder 1.5%, pH 7.0-7.2); cultivated at 30°C for 48 hours, picked a single colony, stored in glycerol, and used it as the strain to be tested. 58 strains were isolated and purified by dilution plating method as the strains to be tested.

[0028] 2. Screening of fusarium wilt biocontrol strains

[0029] (1) Preliminary screening by confrontation method

[0030] The tested strains were activated on LB plates and cultured at 30°C for 48 hours.

[0031] Fusarium oxysporum FJAT-30512 (Chen Qianqian, Liu Bo, Liu Guohong, Che Jianmei, Gong Haiyan. Study on the Diversity of Geo...

Embodiment 2

[0055] Identification of embodiment 2 antagonistic strains

[0056] 1. Morphological characteristics

[0057] The Bacillus sp. FJAT-47582 was activated on the LB plate, and the colony shape, size, color, transparency, protrusion and edge of the strain were observed after 48 hours.

[0058] The colony characteristics of strain FJAT-47582 are as follows figure 1 As shown, the colonies are large, round, light yellow, rough and dry, raised, with irregular edges, and opaque.

[0059] 2. 16S rRNA gene sequence analysis

[0060] Genomic DNA of strain FJAT-47582 was extracted by Tris-saturated phenol method, PCR amplification was carried out using 16S rRNA gene general primers 27F and 1492R, and the PCR reaction procedure was referred to the literature of Zheng Xuefang et al. (Zheng Xuefang, Liu Bo, Zhu Yujing, et al. Tomato bacterial wilt Screening and identification of biocontrol Bacillus [J]. Chinese Journal of Biological Control, 2016, 32(5): 657-665.), the PCR product was sent...

Embodiment 3

[0062] The inhibitory effect of embodiment 3 bacillus to Xanthomonas rugosa

[0063] Inoculate the FJAT-47582 strain on the LB plate, then pick a single bacterial colony and transfer it to 10mL LB liquid medium, 170r min -1 , cultured at 30°C for 48 hours, and counted by a hemocytometer, the colony density of FJAT-47582 reached 2.576×10 8 cfu mL -1 . The FJAT-47582 fermentation broth was centrifuged at 4000 rpm for 8 minutes, the supernatant was taken, and the bacterial cells were discarded to obtain a bacteriostatic solution.

[0064] 1mL of the bacterial suspension of Xanthomonas rugosa FJAT-10151 (preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, preservation number CGMCC NO.10271) (colony density 4.224×10 8 cfu mL -1 ) mixed with 200mL of 50°C NA semi-solid medium (3g of beef extract, 5g of peptone, 10g of glucose, 9g of agar, and 1L of water), draw 4mL and pour it into the prepared NA solid medium (3g of bee...

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Abstract

The invention provides Bacillus for antagonizing blight and application thereof, wherein the strain has a scientific name of Bacillus siamensis FJAT-47582. The Bacillus siamensis FJAT-47582 is preserved in the China General Microbiological Culture Collection Center (CGMCC), with the preservation number of CGMCC NO. 15947. The Bacillus of the invention can not only inhibit fusarium oxysporum, xanthomonas axonopodis and other plant pathogenic bacteria, but also improve the vitality of plant seeds. The bacillus can be used for preventing and treating blight, and is a biocontrol bacterium being environment-friendly, economic and effective in terms of disease prevention and control.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a Bacillus strain antagonizing Fusarium wilt and its application in antagonizing plant pathogenic bacteria and improving seed vigor. Background technique [0002] Fusarium wilt is a systemic infection disease, which is a common soil-borne disease of plants such as lyme, soybean, wheat, tomato, cotton, strawberry, etc. It is an increasingly prominent problem in the United States, Canada, Australia, and China. Due to Fusarium wilt, the leaves become smaller, lack luster, the branches are dwarfed, the fruits are thin, or the leaves turn yellow and wilt. In severe cases, the plants gradually die. The main pathogenic species of fusarium wilt are Fusarium oxysporum and Fusarium solani. The control methods include chemical reagents, fungicides, biological control, phytotherapeutic drugs, optimized fertilization, etc., and the use of biocontrol bacteria to control wilt Dise...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G13/00A01C1/00A01P3/00A01P21/00C12R1/07
CPCC12N1/20A01G13/00A01C1/00C12R2001/07C12N1/205
Inventor 朱育菁郑梅霞陈峥刘波许炼潘志针肖荣凤邓文琼李慧敏
Owner 福建省农业科学院农业生物资源研究所
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