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Bacillus strain with antagonistic action on root rot

A technology of Bacillus and Bacillus subtilis, applied in the field of microorganisms, can solve the problem of poor control effect on plant root rot

Inactive Publication Date: 2017-11-24
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these bacteria do not have a good control effect on plant root rot

Method used

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  • Bacillus strain with antagonistic action on root rot
  • Bacillus strain with antagonistic action on root rot
  • Bacillus strain with antagonistic action on root rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Screening of antagonistic strains

[0017] 1. Soil sample collection and Bacillus isolation and purification

[0018] The grass root soil samples collected from Hoh Xil were air-dried and ground to make a soil suspension, which was then properly diluted with sterile water and coated on an NA plate; cultivated at 30°C for 72 hours, picked a single colony, and preserved it in glycerol as the strain to be tested . 63 strains were isolated and purified by dilution plating method as the strains to be tested.

[0019] 2. Screening of biocontrol strains of Fusarium oxysporum

[0020] (1) Preliminary screening by confrontation method

[0021] The test strain tomato host Fusarium oxysporum FJAT-30512 (preserved in the Institute of Agricultural Biological Resources, Fujian Academy of Agricultural Sciences), in beef extract peptone medium (NA: beef extract 3g, peptone 5g, glucose 10g, agar powder 1.7%, pH 7.2-7.4) on the plate, and incubated at 30°C for 48h. Fusariu...

Embodiment 2

[0041] Identification of embodiment 2 antagonistic strains

[0042] 1. Morphological characteristics

[0043] Bacillus FJAT-20265 was activated on the NA plate, and the colony shape, size, color, transparency, protrusion and edge of the strain were observed after 48 hours.

[0044] The colony characteristics of strain FJAT-20265 are as follows figure 1 As shown, the colonies are small, round, milky white, smooth and moist, raised, with neat edges, and opaque.

[0045] 2.16S rRNA gene sequence analysis

[0046] The genomic DNA of the strain FJAT-20265 was extracted according to the operation steps of the bacterial genome extraction kit (generay biotech#GK 1071, Shanghai Jierui Bioengineering Co., Ltd.), and the 16S rRNA gene general primers 27F and 1492R were used for PCR amplification. The PCR reaction procedure was referred to Literature by Zheng Xuefang et al. (Zheng Xuefang, Liu Bo, Zhu Yujing, et al. Screening and Identification of Bacillus Bacillus for the Biocontrol o...

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Abstract

The invention provides a Bacillus strain with antagonistic action on root rot. The Bacillus strain has a scientific name of Bacillus subtilis FJAT-20265 and is preserved in China General Microbiological Culture Collection Center under the accession number of CGMCC No. 14430. The Bacillus subtilis FJAT-20265 screened in the invention has strong inhibitory effect on main pathogenic bacteria of root rot, i.e., Fusarium oxysporum, Fusarium solani and Fusarium moniliforme, and can be specially used for controlling root rot.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Bacillus strain antagonizing root rot. Background technique [0002] Root rot is a systemic infection disease, which is a common soil-borne disease of plants such as lyme, soybean, wheat, tomato, cotton, strawberry, etc. It is an increasingly prominent problem in the United States, Canada, Australia, and China. Due to root rot, the leaves of the plants become smaller, lack luster, the branches are dwarfed, the fruits are thin, or the leaves turn yellow and wilt. In severe cases, the plants gradually die. The main pathogenic populations of root rot are Fusarium oxysporum and Fusarium solani. The control methods include chemical reagents, fungicides, biological control, phytotherapeutic drugs, optimized fertilization, etc., and biocontrol bacteria are used to control Root rot is an important measure for comprehensive control. [0003] A variety of fungi, bact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P3/00C12R1/125
CPCC12N1/205C12R2001/125
Inventor 郑梅霞朱育菁刘波潘志针陈峥
Owner 福建省农业科学院农业生物资源研究所
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