A Shaker Incubator with High Light Energy Utilization Efficiency for Suspension Culture

Abstract

The invention provides a shaking culture box with a high light energy utilization rate for suspension culture. The shaking culture box comprises a box body, a culture frame assembly, a sterilization unit, a temperature control system unit, a light environment control unit and a control module. The culture frame assembly comprises a rack, culture flask fixing plates, lamp panels, a lamp panel lifting module and a shaking module, wherein the culture flask fixing plates are provided with flask containing grooves, the lamp panels are horizontally arranged at the positions, above the culture flask fixing plates, of the rack and are provided with flask holes, and the flask holes are provided with LED light sources. A heat dissipation fan of the temperature control system discharges air towards the culture frame assembly, and an exhaust fan discharges air towards the outside of the box body. When culture is conducted through the culture frame assembly, the shaking module drives the rack to shake, the lamp panel lifting module drives the lamp panels to move to make conical culture flasks located in the flask holes in the lamp panels in a sleeved mode, the LED light sources provide illumination for the culture flasks, and gaps forming heat dissipation air paths are reserved between the flask holes in the lamp panels and the outer walls of the culture flasks. According to the shaking culture box, a customizable light environment can be provided through the LED light sources, and the situation that the temperature of the box is influenced by the heat of the light sources is not likely to occur.

Description

technical field [0001] The invention relates to optical culture equipment, in particular to a shaking bed incubator with high light energy utilization rate for suspension culture. Background technique [0002] Suspension culture refers to a tissue culture system in which single cells and small cell clusters are cultured in a liquid medium that is constantly stirred or shaken. It is a culture method of non-anchorage-dependent cells. Suspension culture is widely used in the production of secondary metabolites and has unparalleled advantages. Usually, the capacity of the standard Erlenmeyer flasks for suspension culture used in the laboratory is 150ml, 200ml, and 250ml, and the outer diameters of the bottles are 69mm, 75mm, and 85mm, respectively. Nowadays, constant temperature shakers are more and more widely used in suspension culture, but when studying the influence of light on suspension culture, many constant temperature shakers on the market cannot meet the experimental ...

AI Technical Summary

Problems solved by technology

At present, the constant temperature shaker on the market has a variety of installation and design of the light source, and none of them can meet the requirements of light cultivation.

Some shakers install light strips on the side, and some shakers install light panels on the top, but none of them can solve the problem of light uniformity; how to design a light source with a better lighting environment for suspension culture is a research direction

[0004] LED light source is a kind of cold light source that has emerged in recent years. It has the advantages of saving electricity and space. Some patents use LED light source for incubators. The light-emitting surface of LED light source is directional and the amount of light is limited. A certain number of LED chips are required It is easy to achieve the light environment required by the incubator when working. However, although the LED light source is a cold light source, it has a certain working temperature. If a large number of LED light sources are used in the incubator, it may make the temperature environment of the incubator difficult to control. How to solve these problems, is also a research direction

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