Paper mulberry active ingredient extraction method and applications thereof in preparation of cosmetics

Abstract

The invention discloses a paper mulberry active ingredient extraction method and applications thereof in preparation of cosmetics. Cellulase and compound protease are applied to loosen and break plant cell walls, reduce mass transfer resistance and accelerate the release of active ingredients, namely flavonoid substances and polyphenols; the slightly alkaline small-molecular water is used for extracting the residual active ingredient from paper mulberry slag; paper mulberry root and stem extracts prepared by utilizing the modern bioengineering technology of micro-filtering membrane purification extracting liquid and the like are rich in antioxidant ingredients and antibacterial and anti-inflammatory ingredients of polyphenols, flavones and the like, and the extraction and separation yield is high; and the paper mulberry active ingredients can be used for preparing cosmetics.

Description

technical field

[0001] The invention relates to a method for extracting active ingredients from plants, in particular to a method for extracting effective ingredients from Lycium barbadensis and its use in preparing cosmetics. Background technique

[0002] Chinese wolfberry, [Latin scientific name] Broussonetia papyrifera (Linn.) L'Hér. ex Vent ;

Alternative names

Examples

Embodiment 1

[0023] The method for extracting active ingredients of lycium barbadensis comprises the following steps:

[0024] 1) First remove the soil and weeds from the leaves of the wolfberry tree, wash the leaves of the wolfberry tree with tap water, dry them in the shade, and then use an ultra-fine mechanical pulverizer to crush the leaves of the wolfberry tree to reach 1000-2000 mesh to obtain the leaf powder of the wolfberry tree;

[0025] 2) According to the weight of wolfberry leaf powder, add 5 times the amount of water to soak the wolfberry leaf powder obtained in step 1), and at the same time add cellulase with the weight of wolfberry powder 1‰ in the heating reaction kettle, adjust the pH to 6-7, The temperature is 40°C, the stirring speed is 35rpm, and the enzymatic hydrolysis is 4hr; after the cellulase enzymatic hydrolysis, add the compound protease with the weight of 1‰ of citrus leaf hydrolysis for 4 hours; after enzymatic hydrolysis with the compound protease, treat at 9...

Embodiment 2

[0031] The method for extracting active ingredients of lycium barbadensis comprises the following steps:

[0032] 1) First remove the soil and weeds from the leaves of the wolfberry tree, wash the leaves of the wolfberry tree with tap water, dry them in the shade, and then use an ultra-fine mechanical pulverizer to crush the leaves of the wolfberry tree to reach 1000-2000 mesh to obtain the leaf powder of the wolfberry tree;

[0033] 2) According to the weight of the wolfberry leaf powder, add 8 times the amount of water to soak the wolfberry leaf powder obtained in step 1), and at the same time add cellulase with a weight of 3‰ of the wolfberry powder in the heating reaction kettle, adjust the pH to 6-7, The temperature is 45°C, the stirring speed is 30rpm, and the enzymatic hydrolysis is 3hr; after the cellulase enzymatic hydrolysis, add the compound protease with the weight of 3‰ of citrus leaf, adjust the pH to 6-7, the temperature is 45°C, the stirring speed is 30rpm, hyd...

Embodiment 3

[0039] The method for extracting active ingredients of lycium barbadensis comprises the following steps:

[0040] 1) First remove the soil and weeds from the leaves of the wolfberry tree, wash the leaves of the wolfberry tree with tap water, dry them in the shade, and then use an ultra-fine mechanical pulverizer to crush the leaves of the wolfberry tree to reach 1000-2000 mesh to obtain the leaf powder of the wolfberry tree;

[0041] 2) According to the weight of the wolfberry leaf powder, add 10 times the amount of water to soak the wolfberry leaf powder obtained in step 1), and at the same time add cellulase with a weight of wolfberry powder 5‰ in the heating reaction kettle, adjust the pH to 6-7, The temperature is 50°C, the stirring speed is 20rpm, and the enzymatic hydrolysis is 2hr; after the cellulase enzymatic hydrolysis, add the compound protease with the weight of 5‰ of citrus leaves, adjust the pH to 7, the temperature is 50°C, the stirring speed is 20rpm, and the en...

technical field

[0001] The invention relates to a method for extracting active ingredients from plants, in particular to a method for extracting effective ingredients from Lycium barbadensis and its use in preparing cosmetics. Background technique

[0002] Chinese wolfberry, [Latin scientific name] Broussonetia papyrifera (Linn.) L'Hér. ex Vent ;

Alternative names

Examples

Embodiment 1

[0023] The method for extracting active ingredients of lycium barbadensis comprises the following steps:

[0024] 1) First remove the soil and weeds from the leaves of the wolfberry tree, wash the leaves of the wolfberry tree with tap water, dry them in the shade, and then use an ultra-fine mechanical pulverizer to crush the leaves of the wolfberry tree to reach 1000-2000 mesh to obtain the leaf powder of the wolfberry tree;

[0025] 2) According to the weight of wolfberry leaf powder, add 5 times the amount of water to soak the wolfberry leaf powder obtained in step 1), and at the same time add cellulase with the weight of wolfberry powder 1‰ in the heating reaction kettle, adjust the pH to 6-7, The temperature is 40°C, the stirring speed is 35rpm, and the enzymatic hydrolysis is 4hr; after the cellulase enzymatic hydrolysis, add the compound protease with the weight of 1‰ of citrus leaf hydrolysis for 4 hours; after enzymatic hydrolysis with the compound protease, treat at 9...

Embodiment 2

[0031] The method for extracting active ingredients of lycium barbadensis comprises the following steps:

[0032] 1) First remove the soil and weeds from the leaves of the wolfberry tree, wash the leaves of the wolfberry tree with tap water, dry them in the shade, and then use an ultra-fine mechanical pulverizer to crush the leaves of the wolfberry tree to reach 1000-2000 mesh to obtain the leaf powder of the wolfberry tree;

[0033] 2) According to the weight of the wolfberry leaf powder, add 8 times the amount of water to soak the wolfberry leaf powder obtained in step 1), and at the same time add cellulase with a weight of 3‰ of the wolfberry powder in the heating reaction kettle, adjust the pH to 6-7, The temperature is 45°C, the stirring speed is 30rpm, and the enzymatic hydrolysis is 3hr; after the cellulase enzymatic hydrolysis, add the compound protease with the weight of 3‰ of citrus leaf, adjust the pH to 6-7, the temperature is 45°C, the stirring speed is 30rpm, hyd...

Embodiment 3

[0039] The method for extracting active ingredients of lycium barbadensis comprises the following steps:

[0040] 1) First remove the soil and weeds from the leaves of the wolfberry tree, wash the leaves of the wolfberry tree with tap water, dry them in the shade, and then use an ultra-fine mechanical pulverizer to crush the leaves of the wolfberry tree to reach 1000-2000 mesh to obtain the leaf powder of the wolfberry tree;

[0041] 2) According to the weight of the wolfberry leaf powder, add 10 times the amount of water to soak the wolfberry leaf powder obtained in step 1), and at the same time add cellulase with a weight of wolfberry powder 5‰ in the heating reaction kettle, adjust the pH to 6-7, The temperature is 50°C, the stirring speed is 20rpm, and the enzymatic hydrolysis is 2hr; after the cellulase enzymatic hydrolysis, add the compound protease with the weight of 5‰ of citrus leaves, adjust the pH to 7, the temperature is 50°C, the stirring speed is 20rpm, and the en...