Method for treating antibiotic bacterial residue by using laccase

A technology of antibiotic bacteria residues and antibiotics, which is applied in the field of enzymatic hydrolysis of antibiotic residues of antibiotic bacteria residues and the enzymatic hydrolysis of antibiotic residues of antibiotic bacteria residues by using laccase, can solve the problems of long treatment period, high cost, complicated operation, etc., and achieves convenient follow-up. The effect of processing, short reaction period and simple operation

Inactive Publication Date: 2017-03-15
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is used to solve technical problems such as difficult treatment of antibiotic bacteria residue, high cost, easy to produce secondary pollution, long treatment cycle and complicated operation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Prepare a standard sample of erythromycin with a concentration of 0.001g / mL, and its potency is 920U / mL;

[0031] The preparation concentration is 0.01g / mL suspension of thiorubicin bacteria residue, its original potency is 73.4U / mL, and the pH is adjusted to be 6;

[0032] Add laccase suspension to the thioerythromycin residue suspension, the enzyme concentration is 0.015g / mL, and the enzyme activity is 105U / mL; Residual antibiotics were digested. After digestion, the titer of thioerythromycin bacteria residue was 3.67U / mL, and the titer of thioerythromycin bacteria residue was reduced by 95%.

Embodiment 2

[0034] Prepare a standard sample of erythromycin with a concentration of 0.003g / mL, and its potency is 2760U / mL;

[0035] The preparation concentration is 0.02g / mL suspension of thioerythromycin bacteria residue, its original potency is 220.1U / mL, and the pH is adjusted to be 7;

[0036] Add laccase suspension to the thioerythromycin residue suspension, the enzyme concentration is 0.007g / mL, and the enzyme activity is 49U / mL; Residual antibiotics were digested. After digestion, the titer of thioerythromycin residue was 15.4U / mL, and the titer of thioerythromycin residue was reduced by 93%.

Embodiment 3

[0038] Prepare a standard sample of erythromycin with a concentration of 0.005g / mL, and its potency is 4600U / mL;

[0039] The preparation concentration is 0.03g / mL suspension of thioerythromycin bacteria residue, its original potency is 78.15U / mL, and the pH is adjusted to be 7;

[0040] Add laccase suspension to the thioerythromycin residue suspension, the enzyme concentration is 0.02g / mL, and the enzyme activity is 140U / mL; Residual antibiotics were digested. After digestion, the titer of thioerythromycin residue was 3.9 U / mL, and the titer of thioerythromycin residue was reduced by 95%.

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PUM

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Abstract

The invention provides a method for treating antibiotic bacterial residue by using laccase. According to the method adopting laccase for treating the antibiotic bacterial residue, the antibiotic bacterial residue is erythromycin thiocyanate bacterial residue; and the treating conditions are as follows: the temperature is 40 to 55 DEG C, the pH is 6 to 7, the enzyme concentration is 0.005 to 0.02 g/mL, the treating time is 4 to 6 h, and the titer of the erythromycin thiocyanate bacterial residue is reduced by 90% or higher. The method provided by the invention is simple, convenient and feasible; the treatment cost is low; no secondary pollution is generated; energy is saved; the environment is protected; most of residual antibiotics in the antibiotic bacterial residue can be removed, so that the subsequent treatment of the antibiotic bacterial residue is facilitated; and a certain data basis is provided for enzyme treatment technology of the antibiotic bacterial residue.

Description

technical field [0001] The invention belongs to the field of environmental protection, and in particular relates to a method for enzymatic hydrolysis of residual antibiotic residues of antibiotic bacteria, in particular to a method for enzymatic hydrolysis of residual antibiotic residues of antibiotic bacteria by using laccase. Background technique [0002] my country is a major producer of antibiotics. A large amount of solid waste, namely antibiotic residue, will be produced in the fermentation production of antibiotics. The main components of antibiotic residues are: microbial mycelium that produces specific antibiotics, reagents added during the extraction of antibiotics, incompletely utilized medium, degradation products of the medium, metabolites produced during fermentation, and unknown growth factors and small amounts of residual antibiotics. During the open-air stacking of antibiotic residues, on the one hand, it will cause air pollution and directly affect the li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D3/02
CPCA62D3/02
Inventor 臧立华姬丹丹李肖玲
Owner QILU UNIV OF TECH
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