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Directed rapid screening method for enrichment of broad-spectrum ammonia oxidizing bacteria

A broad-spectrum technology of ammonia oxidizing bacteria, applied in the field of water treatment, can solve the problems of difficulty in forming ecological advantages, poor environmental adaptability, long time period, etc., and achieve stable high ammonia oxidation rate, high ecological stability, and simplified treatment Effects on process and operating costs

Active Publication Date: 2019-06-14
BEIJING UNIV OF TECH
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Problems solved by technology

The problem with this technical route is that a single strain has poor adaptability to the environment, and it is difficult for a single strain of high-efficiency AOB to form an ecological advantage in a complex sewage treatment system where many types of bacteria exist.
However, the time period of this kind of culture method is long, and the ammonia oxidation rate of the system is inhibited by low dissolved oxygen, and the reaction efficiency is low. In terms of actual engineering, this cannot be ideally applied.

Method used

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  • Directed rapid screening method for enrichment of broad-spectrum ammonia oxidizing bacteria

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Experimental program
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Effect test

Embodiment 1

[0026] This culture method has passed a small test, and the device is shown in figure 1 , the effect is remarkable, the specific operation is as follows:

[0027] (1) Using a continuous flow reactor for continuous aeration for 4 days without adding ammonia nitrogen, the raw sludge concentration (MLSS) is 6976mg / L, DO is 1.5-2.0mg / L, and COD is 16.084 mg / L, MLSS changed to 5324mg / L after 4 days, DO content rose to 6.34mg / L, COD fluctuated up and down after a straight rise, and finally stabilized at around 60mg / L. The apparent conditions showed that a large number of heterotrophic bacteria were completely consumed, and then entered the stage of enriching broad-spectrum AOB.

[0028](2) On the basis of step (1), adopt the method of continuous water inflow, by controlling the influent water composition, the temperature in the system is 25°C, the initial DO is between 1.0mg / L and 1.2mg / L, and the pH is 8 ~8.4, providing the best conditions for the growth of AOB. In the initial s...

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Abstract

The invention discloses a method for directionally quick screening and enrichment of broad-spectrum ammonia oxidizing bacteria, belonging to the field of water treatment. By adopting active sludge as a provenance, the structural features of original AOB flora are maintained; with a high-DO method, high efficiency of ammonia oxidization is kept; under a condition of high DO, the growth of NOB is inhibited by use of high FA; and through regular sludge discharge, NOB is eliminated by regular sludge discharge while keeping the system activity under the condition of a high speed, and quick enrichment culture of AOB is realized.

Description

technical field [0001] The invention belongs to the field of water treatment, and particularly relates to directional rapid screening and enrichment of broad-spectrum ammonia oxidizing bacteria. The production of filler and the improvement of ammonia nitrogen oxidation performance in sewage plants have broad application prospects. Background technique [0002] In wastewater treatment, the traditional denitrification process must undergo complete nitrification and denitrification to remove nitrogen in water, that is, ammonia nitrogen in water is oxidized to nitrite, nitrite is oxidized to nitrate, and finally nitrate is denitrified. It is reduced to nitrogen gas under the action of bacteria. But for denitrifying bacteria, NO 2 - and NO 3 - can be used as the final electron acceptor. After the ammonia nitrogen is oxidized to nitrite, the subsequent nitrification is no longer carried out, but the nitrogen is directly removed, that is, the NH 4 + →NO 2 - →N 2 process,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/02C12N1/00C02F3/34C02F3/30
CPCC02F3/307C02F3/34C02F3/342C02F3/348C02F2209/08C02F2209/22C12N1/00C12N1/02
Inventor 杨宏于濛雨张帆苏姗孟琛徐富胡银龙
Owner BEIJING UNIV OF TECH
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