Method for preparing infectious bronchitis positive serum by using SPF chicken

A bronchitis and chicken infectious technology, applied in the field of preparing positive serum for chicken infectious bronchitis, can solve the problems of high lethality, slow weight growth of broiler chickens, and decline in the quantity and quality of laying hens, so as to improve the level of prevention and control , the effect of good application prospects

Pending Publication Date: 2017-03-22
ZHONGCHONG XINNUO BIOTECH TAIZHOU CO LTD
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The disease will slow down the weight gain of broilers and reduce the efficiency of leaving the cage; it will reduce

Method used

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  • Method for preparing infectious bronchitis positive serum by using SPF chicken
  • Method for preparing infectious bronchitis positive serum by using SPF chicken

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] - Preparation of immune antigens

[0035] Dilute chicken infectious bronchitis virus H120 strain and H52 strain 10-100 times with sterile normal saline, inoculate 10-11-day-old SPF chicken embryo allantoic cavity with 0.1ml, and incubate at 37°C for 30-36 hours , discard the dead embryos, put them in the refrigerator at 2-8°C for 6-8 hours, harvest the chicken embryo liquid, mix the harvested infectious bronchitis H120 strain and H52 strain virus liquids in equal amounts (V / V), and make 2.5 times After the dilution treatment, the test is carried out, and after the test is qualified, the chicken infectious bronchitis antigen is used as the immune antigen (the test results are shown in Table 1).

[0036] Table 1 Results of immune antigen detection

[0037]

Embodiment 2

[0039] ——Preparation method of chicken infectious bronchitis virus negative serum:

[0040] 15-day-old SPF chickens were selected and raised in a negative pressure isolator. Blood was collected aseptically and serum was collected for testing. After passing the test, adult chickens had negative serum for infectious bronchitis. The test results are shown in Table 2.

Embodiment 3

[0042] ——Preparation of Positive Sera for Chicken Infectious Bronchitis Virus

[0043] (1) Primary immunization: chicken infectious bronchitis antigen immunization, each chicken 0.1 ~ 0.2ml nasal drop.

[0044] (2) After 15 days, collect 2ml of blood by means of subwing vein blood collection, separate the serum, and measure the HI titer. The detection antibody is 2 2 .

[0045] (3) The second immunization: immunize with chicken infectious bronchitis live vaccine, eye drops and nasal drops.

[0046] (4) After 15 days, collect 2ml of blood by means of subwing vein blood collection, separate the serum, and measure the HI titer. The detection antibody is 2 7 .

[0047] (5) The third immunization: immunize with chicken infectious bronchitis live vaccine, and apply eye drops and nasal drops.

[0048] (6) After 15 days, collect 2ml of blood by means of subwing vein blood collection, separate the serum, and measure the HI titer. The detection antibody is 2 9 .

[0049] (7) The f...

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Abstract

The invention discloses a method for preparing infectious bronchitis positive serum by using SPF chicken. The method is characterized by comprising immune animal selection, immune inoculation mode, immune time interval, immune frequency, chicken blood acquisition, serum preparation and examination. According to the invention, positive serum is prepared by infectious bronchitis antigen and live vaccine immunity, the positive serum is a basic guarantee for accurately diagnosing the infectious bronchitis, and accurately evaluating the immunization monitoring and vaccine immunization effect; can increase the prevention and control level of the infectious bronchitis; provides powerful technical support for monitoring the infectious bronchitis and evaluating the quality of the relative biological product used for animals, and has good application prospect.

Description

technical field [0001] The invention relates to a method for preparing chicken infectious bronchitis positive serum by using SPF chickens, and belongs to the technical field of veterinary biological products. Background technique [0002] Chicken infectious bronchitis (Infection bronchitis) is an acute and highly contagious respiratory infectious disease of chickens caused by infectious bronchitis virus (Infection bronchitis virus). Breathe, sneeze. It mainly affects the respiratory tract and kidneys of poultry, and some serotypes can also cause lesions in digestive system organs such as glandular stomach. The disease will slow down the weight gain of broilers and reduce the efficiency of leaving the cage; it will reduce the quantity and quality of laying hens; it will cause stunted chicks and a high lethality rate. It is distributed all over the world, and it is very harmful to the poultry breeding industry, often causing serious economic losses. [0003] In order to red...

Claims

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Application Information

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IPC IPC(8): C07K16/10C07K16/06
CPCC07K16/06C07K16/10
Inventor 刁小龙张家铭李晨曦缪月云
Owner ZHONGCHONG XINNUO BIOTECH TAIZHOU CO LTD
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