Preparation method of fermented tobacco leaf extract capable of reducing irritation and application of fermented tobacco leaf extract to recombinant tobacco leaves

A technology of tobacco leaf extract and tobacco leaf, which is applied in the field of recombined tobacco leaf additives, which can solve the problems of uncomfortable taste, heavy miscellaneous gas, and large irritation, and achieve the effects of reducing smoke irritation, improving large irritation, and good sweetness

Inactive Publication Date: 2017-03-22
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the taste-absorbing defects such as heavy miscellaneous gas, high irritation and uncomfortable taste in the current recombined tobacco leaves, the present invention provides a preparation method of fermented tobacco leaf extract with reduced irritation and its application in recombined tobacco leaves

Method used

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  • Preparation method of fermented tobacco leaf extract capable of reducing irritation and application of fermented tobacco leaf extract to recombinant tobacco leaves
  • Preparation method of fermented tobacco leaf extract capable of reducing irritation and application of fermented tobacco leaf extract to recombinant tobacco leaves

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Effect test

Embodiment 1

[0035] A preparation method of fermented tobacco leaf extract that reduces stimulation, it comprises the following steps:

[0036] 1) Crush the tobacco leaves to 80 mesh, then reflux extract the tobacco powder and water at a mass ratio of 1:50 for 3 hours, then filter, repeat the reflux once to extract the filtrate, and combine the filtrates to obtain the tobacco leaf extract, and sterilize it;

[0037] 2) Weighing 80 parts of tobacco leaf extract, 20 parts of Grifola frondosa extract and 10 parts of lychee core extract by weight, and mix them evenly to obtain a mixed extract;

[0038] 3) Activation of strains: inoculate Raoultella planticola VP4-4 CCTCC NO:M2012005 on the slant medium of Raoultella planticola, at 38°C, after static culture for 24 hours, the activated strains were obtained, and placed in 4 ℃ refrigerator; wherein, Raoultella vegetative slant culture medium is: wort: 120mL, sucrose: 20g / L, agar: 15g / L.

[0039] 4) Preparation of Raoultella phyticum seed cultur...

Embodiment 2

[0046] A preparation method of fermented tobacco leaf extract 2 that reduces stimulation, it comprises the following steps:

[0047] 1) Pulverize the tobacco leaves to 60 mesh, then reflux extract the tobacco powder and water at a mass ratio of 1:40 for 3 hours, then filter, repeat the reflux extraction filtrate 3 times, and combine the filtrates to obtain the tobacco leaf extract, and sterilize it;

[0048] 2) Weighing 60 parts of tobacco leaf extract, 30 parts of Grifola frondosa extract and 10 parts of lychee core extract by weight, and mix them uniformly to obtain a mixed extract;

[0049] 3) Strain activation: Inoculate Raoultella planticola VP4-4 CCTCC NO:M2012005 on the Raoultella planticola slant culture medium at 30°C for 72 hours to obtain activated strains, and place in 4 ℃ refrigerator; wherein, Raoultella vegetative slant culture medium is: wort: 120mL, sucrose: 20g / L, agar: 15g / L;

[0050] 4) Preparation of Raoultella vegetative seed culture solution: Inoculate ...

Embodiment 3

[0057] A preparation method of fermented tobacco leaf extract 3 that reduces stimulation, it comprises the following steps:

[0058] 1) Crush the tobacco leaves to 50 mesh, then reflux extract the tobacco powder and water at a mass ratio of 1:20 for 1 hour, then filter, repeat the reflux extraction filtrate once, and combine the filtrates to obtain the tobacco leaf extract, and sterilize it;

[0059] 2) Weighing 70 parts of tobacco leaf extract, 35 parts of Grifola frondosa extract and 15 parts of lychee core extract by weight, and mix them uniformly to obtain a mixed extract;

[0060] 3) Activation of strains: inoculate Raoultella planticola VP4-4 CCTCC NO:M2012005 on the slant culture medium, at 25°C, after static culture for 24 hours, the activated strains were obtained, and placed in the refrigerator; among them, the slant The medium is: wort juice: 120mL, sucrose: 20g / L, agar: 15g / L.

[0061] 4) Preparation of Raoultella vegetative seed culture solution: Inoculate the ac...

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Abstract

The invention discloses a preparation method of fermented tobacco leaf extract capable of reducing irritation and application of the fermented tobacco leaf extract to recombinant tobacco leaves. The method comprises the following steps: mixing a raoultella planticola seed culture solution and a bacillus methylotrophicus seed culture solution at the weight ratio of 1 to (1-3) to obtain a mixed bacterium solution; inoculating the mixed bacterium solution into a mixed extracting solution according to the volume ratio of 0.5%-2% and fermenting to obtain a fermented solution; and after filtering, decompressing and concentrating to obtain extract with the relative density of 1.0522-1.0833, so as to obtain the fermented tobacco leaf extract capable of reducing the irritation. According to the preparation method disclosed by the invention, the mixed extracting solution formed by a tobacco leaf extracting solution, a grifola frondosa extracting solution and a lychee seed extracting solution is used as a carbon source, a nitrogen source and a substrate and is a source substance of cigarettes; a microbial enzyme system can easily utilize the mixed extracting solution to ferment to obtain a cigarette flavor; and the method disclosed by the invention is simple in technological process, and the raw materials are original tobacco leaves of cigarette products, so that the method has an industrialized application prospect.

Description

technical field [0001] The invention relates to the technical field of recombined tobacco leaf additives, in particular to a method for preparing fermented tobacco leaf extract with reduced irritation and its application in recombined tobacco leaves. Background technique [0002] Reconstituted tobacco leaves, also known as reconstituted tobacco leaves or homogeneous tobacco leaves, are mainly composed of tobacco powder, chips, tobacco stems or low-grade tobacco leaves with adhesives and other additives. The existing domestic papermaking thin sheet production process extracts the water-soluble substances in tobacco leaves and tobacco stems with water, and after solid-liquid separation, the solid part is pulped and then made into a paper base, while the liquid part is concentrated and then coated onto the paper. basically. This production process is largely just a physical reorganization of raw materials. As far as its chemical composition is concerned, the recombined tobacc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/02C12P1/04C12R1/01C12R1/07A24B3/14A24B15/24
CPCC11B9/025A24B3/14A24B15/241C11B9/022C12P1/04
Inventor 魏敏陈义坤罗诚浩李冉宋旭艳
Owner HUBEI CHINA TOBACCO IND
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