GAD gene for increasing stress tolerance of lactic acid bacteria and application thereof
A lactic acid bacteria, stress-tolerant technology, applied in application, genetic engineering, plant genetic improvement and other directions, to achieve the effect of improving survival rate, improving low temperature stress tolerance and acid stress tolerance
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0027] The technical solutions of the present invention will be further explained below through specific implementations in conjunction with the drawings.
[0028] 1. Construction of recombinant expression strain:
[0029] Amplify the CsGAD gene sequence cloned by the inventors from tea leaves in the early stage, and connect it to the expression vector pNZ8148 of Lactococcus lactis to obtain the recombinant plasmid pNZ8148-CsGAD, and then electrotransform it into the host bacteria L.lactisNZ9000, The recombinant strain L.lactis NZ9000-CsGAD was obtained (the lactic acid bacteria L.lactisNZ9000 and the expression plasmid vector pNZ8048 were purchased from Hunan Changsha Yingrun Biotechnology Co., Ltd.). The detailed steps are as follows:
[0030] (1) According to the obtained tea tree GAD gene sequence, the sequence is as shown in SEQ ID NO. 1. Use Primer 5.0 software to design a pair of primers at the 5'and 3'of the sequence:
[0031] CsGAD-up CCG CCATGG ATGGTTCTCTCAAAGATTGC NcoI r...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap