GAD gene for increasing stress tolerance of lactic acid bacteria and application thereof
A lactic acid bacteria, stress-tolerant technology, applied in application, genetic engineering, plant genetic improvement and other directions, to achieve the effect of improving survival rate, improving low temperature stress tolerance and acid stress tolerance
Active Publication Date: 2017-03-22
ANHUI AGRICULTURAL UNIVERSITY
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The invention discloses a GAD (Glutamic Acid Decarboxylase) gene for increasing stress tolerance of lactic acid bacteria. The GAD gene is characterized in that a gene sequence is shown as SEQ ID NO.1. The gene can be used for increasing the cold stress resistance and acid stress resistance of the lactic acid bacteria. A method comprises the following steps: introducing a recombinant plasmid containing the GAD gene into the lactic acid bacteria and excessively expressing the glutamic acid decarboxylase CsGAD for compounding gamma-aminobutyric acid in lactic acid bacteria, thereby increasing the cold stress resistance and acid stress resistance of the lactic acid bacteria. Thus, the survival rate of the lactococcus lactis under low-temperature and acid environments can be increased, the GAD gene can be used for developing and applying various healthcare foods and an excellent guarantee can be supplied for the industrial production.
Application Domain
Technology Topic
Increased stress tolerancePlasmid +10
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