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A kind of seedling test-tube propagation method of broad-leaved orchid

A technology of test tube propagation and orchid orchid, applied in the field of plant tissue culture, can solve the problems of difficult germination, long cycle, high cost, etc., and achieve the effects of high seed germination rate, good seedling quality, and fast seedling growth

Inactive Publication Date: 2018-08-24
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the seedlings of P. latifolia are mainly propagated by division, but there are problems of low reproduction efficiency, high cost, and long cycle, which cannot meet the needs of large-scale commercial planting of P. laxa.
In addition, due to the incomplete development of the embryo, it is extremely difficult to germinate in the natural state.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Collection of explants: the mature and uncracked fruits of Orchid latifolia were collected from the field as explants.

[0041] (2) Aseptic germination: the fruit described in step (1) was soaked in 75% ethanol for 2 minutes, rinsed with sterile water for 3 times, and then sterilized in 0.1% mercuric solution for 10 minutes, rinsed with sterile water for 5 times Afterwards, the fruit is cut open, and the powdered embryo is inoculated on a sterile germination medium and cultured for 93 days to obtain a protocorm, the contamination rate is 5.9%, and the seed germination rate is 100%. The sterile germination medium is: 1.0g / L Huabao No. 1 (HYPONeX 1)+0.5mg / L NAA+18g / L sucrose+4.0g / L agar+55ml / L coconut juice, pH is 5.6.

[0042](3) Protocorm proliferation: the protocorm obtained in step (2) was inoculated into the protocorm proliferation medium and cultivated for 30 days to proliferate to obtain a large number of protocorm-like protocorms, and the multiplication factor...

Embodiment 2

[0048] (1) Collection of explants: the mature and uncracked fruits of Orchid latifolia were collected from the field as explants.

[0049] (2) Aseptic germination: the fruit described in step (1) is soaked in 75% ethanol for 1 minute, rinsed with sterile water for 3 times, then disinfected in 0.1% mercuric solution for 10 minutes, rinsed with sterile water for 5 times The fruit is cut open, the powdered embryo is inoculated on the aseptic germination medium and cultivated for 100 days to obtain the protocorm, the pollution rate is 10%, and the germination rate of the seed reaches more than 95%. The sterile germination medium is: 1.5g / L Huabao No. 1 (HYPONeX 1)+0.4mg / L NAA+30g / L sucrose+5.0g / L agar+75ml / L coconut juice, pH is 5.4.

[0050] (3) Protocorm proliferation: the protocorm obtained in step (2) was inoculated into the protocorm proliferation medium and cultivated for 38 days to proliferate to obtain a large number of protocorm-like protocorms, and the multiplication coe...

Embodiment 3

[0056] (1) Collection of explants: the mature and uncracked fruits of Orchid latifolia were collected from the field as explants.

[0057] (2) Aseptic germination: the fruit described in step (1) was soaked in 75% ethanol for 5 minutes, rinsed with sterile water for 5 times, and disinfected in 0.1% mercuric solution for 30 minutes, rinsed with sterile water for 7 times The fruit is cut open, the powdered embryo is inoculated on the aseptic germination medium and cultivated for 110 days to obtain the protocorm, the pollution rate is lower than 3%, and the seed germination rate reaches more than 90%. The sterile germination medium is: 2.0g / L Huabao No. 1 (HYPONeX 1)+1.0mg / L NAA+30g / L sucrose+6.0g / L agar+100ml / L coconut juice, pH is 5.8.

[0058] (3) Protocorm proliferation: inoculate the protocorm obtained in step (2) into the protocorm proliferation medium and cultivate it for 42 days to proliferate and obtain a large amount of protocorm-like protocorms with a multiplication fa...

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Abstract

The invention discloses a tube propagation method of seedlings of Epigeneium amplum. The method comprises the following steps: a step 1: fruits of Epigeneium amplum which are developed and matured as well as do not crack are collected, and the fruits are used as explants; a step 2: aseptic germination of powdery embryos in explants is carried out in order to obtain protocorm, a medium used for aseptic germination comprises the following components: 0.5-2.0g / L Huabao number 1, 0.2-1.0mg / L NAA, 15-30g / L cane sugar, 3.5-6.0g / L agar, and 50-100ml / L coconut milk, and pH value is 5.4-5.8; a step 3: protocorm is propagated in order to obtain protocorm-like bodies; a step 4: protocorm-like bodies are differentiated and cultivated in order to obtain plantlets; a step 5: plantlets are cultivated in order to obtain test-tube plantlets; a step 6: test tube plantlets are transplanted in order to obtain seedlings. The invention aims at providing the tube propagation method of seedlings of Epigeneium amplum, and the method is advantageous in that the method can keep extremely high germination rate.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a test tube propagation method for seedlings of orchid orchids. Background technique [0002] Epigeneium amlum (Lindl.) Summerh. belongs to the genus Epigeneium amlum in the family Orchidaceae. It is the largest species of the plant in the production area of ​​China in the genus. Nearly diamond-shaped, with no pleats on it, it is an easier-to-recognize variety of Orchid orchid. The broad-leaf orchid is mainly distributed in Yunnan, Tibet, Guangxi and other places in my country. It grows in forests at an altitude of 1000-1900 meters or on rocks by streams and tree trunks in mountain forests. In ethnic minority areas such as Yunnan and Tibet, the broad-leaved orchid orchid is often used to treat diseases such as bronchitis, encephalitis, tuberculosis, hepatitis, dysentery, and chronic osteomyelitis. Due to the extensive pharmacological effects of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 梁钧淞杨业容莫昭展韦敏
Owner YULIN NORMAL UNIVERSITY