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Novel process method for purifying mistletoe alkaloid

A process method and alkaloid technology, which is applied in the direction of medical formula, plant raw materials, medical preparations containing active ingredients, etc., can solve the problems of low extraction purity and low extraction rate, and achieve high extraction purity, large adsorption capacity, and separation Obvious effect

Inactive Publication Date: 2017-03-29
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a novel process for purifying mistletoe alkaloids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A novel process for purifying mistletoe alkaloids, the steps are as follows:

[0027] (1) crush the dried mistletoe and pass through a 40-mesh sieve, percolate twice with technical ethanol for 6 hours each time, combine the percolate, concentrate under reduced pressure, and obtain an extract;

[0028] (2) soak above-mentioned medicinal extract with acid water, filter with cotton, obtain filtrate;

[0029] (3) The above filtrate was extracted 3 times with chloroform to obtain a chloroform layer and a water layer, the water layer was alkalized with concentrated ammonia water, extracted 2 times with chloroform, the extracts were combined, and concentrated under reduced pressure to obtain the extract;

[0030] (4) Put the above-mentioned extract through a 20-mesh sieve into the extractor, and introduce CO2 with ethanol as the entrainer from the bottom. 2 , 20Mpa, supercritical extraction at 40°C for 8h, the CO flowing out from the upper part of the extractor 2 Separation ...

Embodiment example 2

[0035] A novel process for purifying mistletoe alkaloids, the steps are as follows:

[0036] (1) crush the dried mistletoe and pass through a 50-mesh sieve, percolate with technical ethanol for 3 times, each time for 7 hours, combine the percolation liquid, concentrate under reduced pressure, and obtain an extract;

[0037] (2) soak above-mentioned medicinal extract with acid water, filter with cotton, obtain filtrate;

[0038] (3) The above filtrate was extracted 4 times with chloroform to obtain a chloroform layer and a water layer, the water layer was alkalized with concentrated ammonia water, extracted 2 times with chloroform, the extracts were combined, and concentrated under reduced pressure to obtain the extract;

[0039] (4) Put the above-mentioned extract through a 30-mesh sieve into the extractor, and feed CO2 with ethanol as the entrainer from the bottom. 2 , 30Mpa, supercritical extraction at 50°C for 9h, the CO flowing out from the upper part of the extractor 2 ...

Embodiment example 3

[0044] A novel process for purifying mistletoe alkaloids, the steps are as follows:

[0045] (1) crush the dried mistletoe and pass through a 60-mesh sieve, percolate with ethanol for 4 times, each time for 8 hours, combine the percolate, concentrate under reduced pressure, and obtain an extract;

[0046] (2) soak above-mentioned medicinal extract with acid water, filter with cotton, obtain filtrate;

[0047] (3) The above filtrate was extracted 3 times with chloroform to obtain a chloroform layer and a water layer, the water layer was basified with concentrated ammonia water, extracted 3 times with chloroform, the extracts were combined, and concentrated under reduced pressure to obtain the extract;

[0048] (4) Put the above-mentioned extract through a 40-mesh sieve into the extractor, and pass into CO2 with ethanol as the entrainer from the bottom. 2 , 40Mpa, supercritical extraction at 60°C for 10h, the CO flowing out from the upper part of the extractor 2 Separation und...

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PUM

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Abstract

The invention discloses a novel process method for purifying mistletoe alkaloid by applying a supercritical fluid assisted membrane separation technology. The adsorptive selectivity of the alkaloid is good, the adsorption and desorption are both quick, and the adsorption capacity is relatively large; and the extraction is convenient and quick, raw material sources are rich, the production cost is low, the separation effect is remarkable, the extraction purity is high, and the alkaloid with the content of more than 98% can be obtained. The shortcomings of relatively low extraction rate and low extraction purity of a conventional method are overcome.

Description

[0001] Technical field: [0002] The invention belongs to the field of natural organic chemistry and relates to a method for purifying alkaloids, in particular to a novel process for purifying mistletoe alkaloids. [0003] Background technique: [0004] Mistletoe is a semi-parasitic evergreen shrub of the family Morusaceae. This genus is distributed in most provinces of my country, and there are more than 30 species. Only one species of mistletoe is recorded in Chinese Pharmacopoeia. The main production area of ​​this species is in the northeast, and it mainly parasitizes on trees such as elm, birch, willow, maple, and poplar. Mistletoe has a long history as a traditional Chinese medicine. It is often used as a medicine for dispelling rheumatism. It is bitter in taste and flat in nature, and it returns to the liver and kidney meridian. In recent years, research on mistletoe has developed rapidly at home and abroad, and new curative effects and applications have been discovere...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185
CPCA61K36/185A61K2236/15A61K2236/33A61K2236/39A61K2236/50A61K2236/53A61K2236/55
Inventor 姚德坤李杨
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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