Space-flight mutation mutant strain Pc-m-6 of pochonia chlamydosporia as well as biocontrol preparation of space-flight mutation mutant strain Pc-m-6 and applications of space-flight mutation mutant strain Pc-m-6 and biocontrol preparation

Abstract

The invention discloses a space-flight mutation mutant strain Pc-m-6 of pochonia chlamydosporia as well as a biocontrol preparation of the space-flight mutation mutant strain Pc-m-6 and applications of the space-flight mutation mutant strain Pc-m-6 and the biocontrol preparation. Pochonia chlamydosporia is loaded to the Shenzhou 8 spacecraft for space-flight mutation, the original bacterial strain not loaded to the Shenzhou 8 spacecraft is taken as the contrast, after preliminary screening, the space-flight mutation mutant strains of pochonia chlamydosporia are obtained and are subjected to the biological detection including the colony growth rate, hypha dry weight, sporulation quantity and sporulation quantity, the salt tolerance and the resistance to resistance are also tested, and finally, one mutant strain Pc-m-6 which is excellent in performance and is suitable for biological prevention and control for root-knot nematode is obtained. The mutant strain has high spore yield, strong pathogenic ability for root-knot nematode eggs, and excellent resistance for benomyl. The invention further provides the biocontrol preparation prepared by adopting the space-flight mutation mutant strain, and the applications of the biocontrol preparation in preventing and treating root-knot nematode.

Description

technical field [0001] The present invention relates to space-flight mutation mutant strain of Pochonia chamydosporia=Verticillium chlamydosporium, in particular to the space-flight mutation mutant strain of Pochonia chamydosporia=Verticillium chlamydosporium Pc-m-6 and the biocontrol agent prepared therefrom. The present invention further relates to the application of the mutant strain and the biocontrol preparation prepared from the mutant strain in the control of root-knot nematode, and belongs to the field of screening and application of the mutant strain of P. chlamydoides. Background technique [0002] Pochonia chamydosporia=Verticillium chlamydosporium belongs to Deuterom ycotina Hyphomycetes and is an important nematode-eating fungus (Lin Maosong, Shen Suwen. Verticillium chlamydosporium) Preliminary report on the control of root-knot nematode incognita [J]. Biological Control Bulletin, 1994, 10(1):7-10) can effectively control root-knot nematode (Meloidogyne incogni...

AI Technical Summary

Problems solved by technology

[0004] As a biopesticide, P. chlamydoides, like other plant nematode biocontrol bacteria, has many advantages that chemical pesticides cannot match, but at the same time, it also has slow growth, is greatly affected by the environment, unstable control effect and self-stress resistance. Therefore, it is necessary to carry out mutagenesis on the nematode biocontrol fungus P. chlamydoides, and screen out mutant strains with high spore production, strong nematicidal ability, and good resistance to pesticides, so that they can Play a better role in controlling plant nematodes

Images