Space-flight mutation mutant strain Pc-m-6 of pochonia chlamydosporia as well as biocontrol preparation of space-flight mutation mutant strain Pc-m-6 and applications of space-flight mutation mutant strain Pc-m-6 and biocontrol preparation

A puconia, aerospace mutagenesis technology, applied in the application, biocide, fungicide and other directions, can solve the problems of unstable control effect, poor self-resistance, slow growth and so on

Active Publication Date: 2017-04-19
四川金珠生态农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As a biopesticide, P. chlamydoides, like other plant nematode biocontrol bacteria, has many advantages that chemical pesticides cannot match, but at the same time, it also has slow growth, is greatly affected by the environment, unstable control effect and self-stress resistance. T

Method used

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  • Space-flight mutation mutant strain Pc-m-6 of pochonia chlamydosporia as well as biocontrol preparation of space-flight mutation mutant strain Pc-m-6 and applications of space-flight mutation mutant strain Pc-m-6 and biocontrol preparation
  • Space-flight mutation mutant strain Pc-m-6 of pochonia chlamydosporia as well as biocontrol preparation of space-flight mutation mutant strain Pc-m-6 and applications of space-flight mutation mutant strain Pc-m-6 and biocontrol preparation
  • Space-flight mutation mutant strain Pc-m-6 of pochonia chlamydosporia as well as biocontrol preparation of space-flight mutation mutant strain Pc-m-6 and applications of space-flight mutation mutant strain Pc-m-6 and biocontrol preparation

Examples

Experimental program
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Effect test

preparation example Construction

[0027] The preparation of embodiment P. chlamydobacterium wettable powder

[0028] One, the preparation of P. chlamydoides spore powder

[0029] (1), preparation of fermentation broth

[0030] 1. After activating the spaceflight mutagenesis mutant strain of P. chlamydoides, carry out shake flask seed culture;

[0031] The composition of shake flask seed culture medium (by mass percentage):

[0032]

[0033]

[0034] Liquid fermentation conditions: put 200ml of seed medium into a 500ml triangular flask, inoculate the spore suspension of Proconia chlamydoides after autoclaving, the inoculation amount is 1.5-3%, place at 24-28°C, shaker 150- 200r / min, cultivate for 18-36h.

[0035] 2. Secondary liquid expansion culture (by mass percentage):

[0036] Medium composition for secondary liquid expansion culture:

[0037]

[0038] The above-mentioned secondary liquid expansion medium is sterilized in place in a fermenter;

[0039] Liquid fermentation conditions: fermenta...

experiment example 1

[0118] Experimental example 1 Field experiment of controlling root-knot nematode of pepper with wettable powder prepared by spaceflight mutagenesis mutant strain Pc-m-6 of P. chlamydoides

[0119] 1 Test materials and methods

[0120] 1.1 Bacteria to be tested

[0121] Test bacterial agent: wettable powder (prepared in Example 1) prepared with P.

[0122] Control bacterial agent: wettable powder (prepared according to the method of Example 1) prepared with the original strain of Proconia chlamydota (without aerospace mutagenesis).

[0123] 1.2 Experimental crops

[0124] Pepper trees grown in pepper growing areas.

[0125] 1.3 Experimental design

[0126] The experimental site was set in a pepper planting area with a large and uniform nematode population, and the population of the second instar larvae of the root-knot nematode was 20-30 per 100g of soil in the pre-medication survey.

[0127] The experiment is divided into 3 groups, test 1 group, test 2 group and clean wat...

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Abstract

The invention discloses a space-flight mutation mutant strain Pc-m-6 of pochonia chlamydosporia as well as a biocontrol preparation of the space-flight mutation mutant strain Pc-m-6 and applications of the space-flight mutation mutant strain Pc-m-6 and the biocontrol preparation. Pochonia chlamydosporia is loaded to the Shenzhou 8 spacecraft for space-flight mutation, the original bacterial strain not loaded to the Shenzhou 8 spacecraft is taken as the contrast, after preliminary screening, the space-flight mutation mutant strains of pochonia chlamydosporia are obtained and are subjected to the biological detection including the colony growth rate, hypha dry weight, sporulation quantity and sporulation quantity, the salt tolerance and the resistance to resistance are also tested, and finally, one mutant strain Pc-m-6 which is excellent in performance and is suitable for biological prevention and control for root-knot nematode is obtained. The mutant strain has high spore yield, strong pathogenic ability for root-knot nematode eggs, and excellent resistance for benomyl. The invention further provides the biocontrol preparation prepared by adopting the space-flight mutation mutant strain, and the applications of the biocontrol preparation in preventing and treating root-knot nematode.

Description

technical field [0001] The present invention relates to space-flight mutation mutant strain of Pochonia chamydosporia=Verticillium chlamydosporium, in particular to the space-flight mutation mutant strain of Pochonia chamydosporia=Verticillium chlamydosporium Pc-m-6 and the biocontrol agent prepared therefrom. The present invention further relates to the application of the mutant strain and the biocontrol preparation prepared from the mutant strain in the control of root-knot nematode, and belongs to the field of screening and application of the mutant strain of P. chlamydoides. Background technique [0002] Pochonia chamydosporia=Verticillium chlamydosporium belongs to Deuterom ycotina Hyphomycetes and is an important nematode-eating fungus (Lin Maosong, Shen Suwen. Verticillium chlamydosporium) Preliminary report on the control of root-knot nematode incognita [J]. Biological Control Bulletin, 1994, 10(1):7-10) can effectively control root-knot nematode (Meloidogyne incogni...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 汪来发孟繁丽王曦茁薛寒田国忠苟大平
Owner 四川金珠生态农业科技有限公司
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