Vitamin B2 for gestational diabetes
A technology for gestational diabetes and vitamins, applied in the field of nutritional compositions for mothers, capable of solving problems such as impaired glucose tolerance and increased risk
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Embodiment 1
[0163] Contains with inositol, vitamins B6, B12 and D and Lactobacillus rhamnosus GG 1 Bifidobacterium lactis BB12 2 Exemplary compositions of combined vitamin B2 are set forth in Table 1.
[0164] The compositions in Table 1 are used in a nutritional supplement in powder form intended to be sprayed onto food.
[0165] Table 1: Composition of Example 1
[0166]
[0167]
[0168] 1) The strain deposited under the name CGMCC 1.3724
[0169] 2) The strain deposited under the name CNCM I-3446
[0170] The composition may be provided as a kit containing the probiotic powder in one sachet and all other ingredients in a second sachet.
Embodiment 2
[0172] A powdered milk beverage for pregnant women to be reconstituted in water is provided, consisting of 30 g of milk powder per serving mixed with the ingredients listed in Table 1 in half the amount mentioned in Table 1. The composition is administered to the pregnant woman twice daily during at least the last trimester of pregnancy.
Embodiment 3
[0174] The effect of vitamin B2 on glucose uptake in L6 differentiated muscle cells was measured after short-term (2 hours) and long-term (24 hours) culture.
[0175] Component G refers to vitamin B2, component k refers to 1,25-dihydroxyvitamin D3, and component J refers to zinc chloride.
[0176] Component G was tested at 1 [mu]M. Component K was tested at 10 nM and component J at 20 μM.
[0177] On the day of the experiment, components were diluted in serum-free medium (DMEM) containing 5.5 mM D-glucose to achieve final test concentrations and 0.1% DMSO.
[0178] L6 myoblasts were maintained in growth medium or DMEM 25 mM glucose supplemented with 10% fetal bovine serum (FBS), 100 U / ml penicillin and 100 μg / ml streptomycin. For glucose uptake studies, cells were cultured in 24-well plates at a density of 1000-1500 cells / well in 0.5 mL of growth medium. Five days after plating, in DMEM 25 mM glucose containing 2% FBS, 100 U / ml penicillin and 100 μg / ml streptomycin at 37 °C...
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