Preparation for preventing and treating pepper blight, its preparation and application

A pepper blight and preparation technology, applied in the application, fungicide, biocide and other directions, can solve the problems of different prevention and control mechanisms, and does not involve the prevention and control of Solanum nigrum stem and leaf pepper blight, etc., and achieves environmental protection, easy availability, and ease of use. Degradation effect

Active Publication Date: 2019-08-30
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After searching the existing technical documents, it was found that Chinese patent publication numbers: CN102415378A, CN103053624A, CN1543801, and CN104920509A respectively describe the use of an alcohol solution of cinnamaldehyde and azone, a mixture of Trichoderma, eugenol and mandipropamid, The method of preventing and treating pepper blight with actinomycetes and garlic bulb juice does not involve the prevention and treatment of pepper blight by the stems and leaves of Solanum nigrum, and the pathogenic bacteria that cause different plant fungal diseases belong to different species of microorganisms, and their control mechanisms are different.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Weigh 100g of the dry weight of the whole plant of Solanum nigrum, crush it, and extract it with 500ml of 30% ethanol solution for 12 hours at a room temperature of 20°C. The substance is fully dissolved, extracted twice with an equal volume of ethyl acetate, and the extracted upper ethyl acetate phase is concentrated to dryness under reduced pressure, which is the nightshade extract; dissolved in distilled water, and added with a final concentration of 1% (mass) to help Tween 80, prepared as a mother liquor with an extract mass concentration of 10% for later use.

[0033] Determination of the effect of Solanum nigrum extract on the growth of Phytophthora capsici by using the mycelial growth rate method: mix the mother liquor with PDA medium to make the final concentration of the extract 1% (mass), shake well, and pour into a diameter of 9cm A flat plate was prepared in a petri dish, and a PDA plate without any agent was used as a blank control. A piece of mycelium of p...

Embodiment 2

[0035] Weigh 200g of fresh Solanum nigrum, and use 500ml of 80% ethanol solution to extract the slurry after grinding or crushing at room temperature of 25°C overnight. Shake to suspend the dry matter, and make it into an aqueous solution for direct use.

[0036] The indoor potting method was used for the prevention experiment of pepper blight. The pepper seedlings of susceptible varieties were selected to be cultivated to the stage of 4 true leaves, and the whole plant of Solanum solanum was sprayed with water. Water treatment was set as a control, and 20 plants were taken each. One day after the spraying, the spraying group and the control group were inoculated by the root irrigation method, and the inoculation volume was 5ml / plant. Put the pepper seedlings in a 35°C incubator for cultivation, and keep the humidity above 80% to promote the disease. According to the national standard of the People's Republic of China GB / T 17980.32-2000 fungicides for the prevention and treat...

Embodiment 3

[0038] Weigh 100g of Solanum nigrum, crush it, and extract it with 1000ml of 50% ethanol solution for 24 hours at a room temperature of 15°C. After the extract is concentrated under reduced pressure, add 100ml of distilled water, place it in an ultrasonic wave to suspend the dry matter, and then Extract once with an equal volume of n-butanol, and concentrate the extracted upper n-butanol phase to dryness under reduced pressure to obtain the Solanum nigrum extract; dissolve it in distilled water, and add tea saponin and SDS to prepare the extract with a concentration of 3% (mass) of water.

[0039] The treatment experiment of capsicum blight was carried out in an indoor pot experiment. Four capsicum seedlings of the susceptible variety at the true leaf stage were first inoculated with the capsicum blight culture solution, and then sprayed with Solanum solanum aqua solution one day later, and treated with clean water as a control. Cultivation method and disease statistics are th...

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PUM

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Abstract

The invention relates to a preparation for prevention and cure of pepper phytophthora blight, and production and an application thereof. The production comprises the steps: crushing a combination of one or more of roots, stems, leaves, flowers and fruits of solanum nigrum l., extracting and concentrating at room temperature with ethanol or an ethanol aqueous solution, and then producing into a water agent. The solanum nigrum l. extract is used as an effective ingredient; in-vitro bacteriostatic tests and seedling stage pot tests prove that the preparation has the control effect on pepper phytophthora blight reaching about 70%. The preparation has the advantages of wide sources of raw materials, low cost, simple technology and environmental-protection process, and can effectively prevent and control pepper phytophthora blight, promotes the production, reduces the amount of chemical pesticides, and is favorable for environmental protection.

Description

technical field [0001] The invention relates to an agricultural fungicide and its preparation technology, in particular to a technology in which a plant extract is used as a main bioactive compound to prevent and treat pepper diseases. Background technique [0002] Pepper blight is caused by Phytophthora capsici ( Phytophthora capsici ) caused by a soil-borne disease, which occurs worldwide and in pepper producing areas in various provinces of China, causing serious damage. Phytophthora capsici not only infects peppers, causing losses in pepper production, but also infects a variety of crops of Solanaceae and Cucurbitaceae. In production, chemical agents such as metalaxyl, benalaxyl, and fofosalum are mainly used to prevent and control pepper blight. However, the control effect is not ideal due to the continuous increase in the resistance of bacteria in recent years. Therefore, it is urgent to adopt new control measures to control pepper blight. [0003] Solanum nigrum( ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/38A01P3/00A01G13/00
CPCA01G13/00A01N65/00A01N65/38Y02A50/30
Inventor 陈佳严准高春生余永廷严理李智敏曾粮斌程毅孙向平薛召东
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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