Bioremediation method for soil polluted by heavy metal lead cadmium and base on kosteletzkya virginica
A cadmium-contaminated soil and bioremediation technology is applied in the field of heavy metal lead-cadmium-contaminated soil bioremediation based on seaside mallow. The effect of reducing stability and promoting absorption
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Embodiment 1
[0029] A method for bioremediation of heavy metal lead and cadmium contaminated soil based on seaside mallow, comprising the steps of:
[0030] Step 1, preparing mixed soil: crush river sand, humus soil and garden soil respectively, mix them evenly according to the mass ratio of 3:3:4, pass through a sieve with a mesh diameter of 2mm, and obtain mixed soil;
[0031] Step 2, preparing nutrient soil: adding effective viable bacteria to every kilogram of mixed soil is 1×10 8 25g of young capsule mycotic agent per g, the effective number of viable bacteria is 3×10 8 Each / mL of Bacillus megaterium agent 20ml, mix well, obtain nutrient soil;
[0032] Step 3, prepare the nutrient bowl: put the nutrient soil into a seedling raising pot with an outer diameter of 9cm and a height of 9cm to obtain a nutrient bowl;
[0033] Step 4, seed pretreatment: select seashore mallow seeds with plump grains and good seed coat color, soak them in water for two days, take them out, and set aside;
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Embodiment 2
[0041] A method for bioremediation of heavy metal lead and cadmium contaminated soil based on seaside mallow, comprising the steps of:
[0042] Step 1, preparing mixed soil: crush river sand, humus soil and garden soil respectively, mix them evenly according to the mass ratio of 3:3:4, pass through a sieve with a mesh diameter of 2mm, and obtain mixed soil;
[0043] Step 2, preparing nutrient soil: adding effective viable bacteria to every kilogram of mixed soil is 1.5×10 8 25g of young capsule mycotic agent per g, the effective number of viable bacteria is 3.5×10 8 Individual / mL Pseudomonas agent 20ml, mix well, obtain nutrient soil;
[0044] Step 3, prepare the nutrient bowl: put the nutrient soil into a seedling raising pot with an outer diameter of 9cm and a height of 9cm to obtain a nutrient bowl;
[0045] Step 4, seed pretreatment: select seashore mallow seeds with plump grains and good seed coat color, soak them in water for two days, take them out, and set aside;
...
Embodiment 3
[0053] A method for bioremediation of heavy metal lead and cadmium contaminated soil based on seaside mallow, comprising the steps of:
[0054] Step 1, preparing mixed soil: crush river sand, humus soil and garden soil respectively, mix them evenly according to the mass ratio of 3:3:4, pass through a sieve with a mesh diameter of 2mm, and obtain mixed soil;
[0055] Step 2, preparing nutrient soil: adding effective viable bacteria to every kilogram of mixed soil is 2×10 8 25g of Moses capsule mold agent per g, the effective number of viable bacteria is 4×10 8 Each / mL rhizobia agent 20ml, mix uniformly, obtain nutrient soil;
[0056] Step 3, prepare the nutrient bowl: put the nutrient soil into a seedling raising pot with an outer diameter of 9cm and a height of 9cm to obtain a nutrient bowl;
[0057] Step 4, seed pretreatment: select seashore mallow seeds with plump grains and good seed coat color, soak them in water for two days, take them out, and set aside;
[0058] Step...
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