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Panebacillus polymyxa KM2501-1 and application thereof

A technology of bacillus and polymyxa, which is applied in the field of agricultural microbiology to achieve the effect of promoting growth

Active Publication Date: 2017-04-26
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The control of Haemonchus contortus mainly relies on some anthelmintics, but due to the widespread drug resistance, new drugs are urgently needed to be developed

Method used

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  • Panebacillus polymyxa KM2501-1 and application thereof
  • Panebacillus polymyxa KM2501-1 and application thereof
  • Panebacillus polymyxa KM2501-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The acquisition of Paenibacillus polymyxa KM2501-1:

[0034] The applicant obtained a strain of Paenibacillus polymyxa KM2501-1 isolated from the rhizosphere soil of the organic complex polluted ranunculus in Hukou County, Jiangxi Province. : Paenibacillus polymyxa KM2501-1, deposit number: CCTCC NO: M 2016720, address: Wuhan University, Wuhan, China. The physiological and biochemical characteristics and culture characteristics of Paenibacillus polymyxa KM2501-1 are shown in Table 1.

[0035] Table 1 Physiological and biochemical characteristics and culture characteristics of Paenibacillus polymyxa KM2501-1

[0036]

[0037]

Embodiment 2

[0039] Preparation of Paenibacillus polymyxa KM2501-1 fermentation broth:

[0040] Streak Paenibacillus polymyxa KM2501-1 on the plate to activate it, take a single colony on the plate and transfer it to a conical flask containing 100 ml of KMB medium, the culture temperature is 28°C; the shaker speed is 180rpm; the culture time is 48h; Paenibacillus polymyxa KM2501-1 fermentation liquid (the present invention or be called for short KM2501-1 fermentation liquid), 8000rpm centrifugal 15min obtains Paenibacillus polymyxa KM2501-1 fermentation supernatant (the present invention or be called for short KM2501-1 fermentation supernatant ) and cells, the cells were resuspended in double distilled water, and the OD 600 The value was adjusted to 0.8 as Paenibacillus polymyxa KM2501-1 bacterial suspension (the present invention or KM2501-1 bacterial suspension for short), which was used in the following examples.

Embodiment 3

[0042] The application of Paenibacillus polymyxa KM2501-1 in the preparation of medicines for treating or preventing plant root-knot nematode disease comprises the following steps:

[0043] Toxicity of KM2501-1 to Meloidogyne incognita

[0044] 1) Carry out virulence assay with 96-well plate, add 20-30 root-knot nematodes incognita to each hole;

[0045] 2) Add 120 μL filter-sterilized Paenibacillus polymyxa KM2501-1 fermentation supernatant (prepared in Example 2) to each well;

[0046] 3) The experiment set up 4 groups:

[0047] KM2501-1 fermentation supernatant group (1×CF): KM2501-1 fermentation supernatant+Meloidogyne incognita;

[0048] 1 / 2 concentration KM2501-1 fermentation supernatant group (1 / 2×CF): 1 / 2 concentration KM2501-1 fermentation supernatant + M. incognita;

[0049] 1 / 4 concentration KM2501-1 fermentation supernatant group (1 / 4×CF): 1 / 4 concentration KM2501-1 fermentation supernatant + M. incognita;

[0050] Control group (CK): KMB medium + M. incognita....

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Abstract

The invention discloses a panebacillus polymyxa KM2501-1 and an application thereof. The strain is preserved in China Center for Type Culture Collection with preservation number of CCTCC NO: M 2016720. A volatile substance, generated from solid-state fermentation of the panebacillus polymyxa KM2501-1 provided by the invention, has a good effect of controlling plant pathogenic nematode, namely root-knot nematode, and animal pathogenic nematode, namely haemonchus contortus; fermented supernatant liquid of the strain has an excellent effect of controlling the root-knot nematode and various pathogenic fungi; meanwhile, the panebacillus polymyxa and metabolites have an excellent property of promoting the growth of tomato plants; at the same time, the fermented supernatant liquid is excellent in effect of inhibiting incubation of root-knot nematode; and the panebacillus polymyxa KM2501-1 is capable of preventing nematodes from various ways, the panebacillus polymyxa KM2501-1 is safe and is excellent in application prospect.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbiology, and in particular relates to Paenibacillus polymyxa KM2501-1 and application thereof. Background technique [0002] Root-knot nematode disease is a soil-borne disease distributed worldwide. Root-knot nematode disease caused by root-knot nematode is one of the most serious damages to vegetables, mainly affecting many common vegetables such as cucumber, tomato and eggplant. And with the adjustment of the planting industry structure and the rapid expansion of the cultivated area of ​​vegetable protection, the incidence of root-knot nematode is serious year by year, and the yield loss reaches 30%-50%. At the same time, root-knot nematode damage has aggravated the occurrence of soil-borne fungal diseases such as fusarium wilt and root rot and some bacterial diseases. Has become a major obstacle to current vegetable production. Therefore, the prevention and treatment of vegetable ro...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P5/00A01P3/00A01P21/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 张吉斌程万里聂啟玉杨景艳蔡珉敏郑龙玉喻子牛胡敏高冲
Owner HUAZHONG AGRI UNIV