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Method for analyzing influence of carbon-nanotube-reinforced silk on fibroin amino acid sequences based on proteomics

A technology of proteomics and carbon nanotubes, applied in the field of affecting the amino acid sequence of silk protein, can solve the problems of low strength, difficult to make judgments, weak resistance to external force damage, etc., achieve less sample usage, improve degumming effect, and accurate high degree of effect

Inactive Publication Date: 2017-04-26
ZHEJIANG SCI-TECH UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Silk is one of the products of ancient Han civilization. It is a continuous long fiber that is solidified by secreting silk liquid when cooked silkworms make cocoons. It is also called "natural silk". It is one of the earliest animal fibers used by humans like wool. Silk has light , thin, soft and other advantages, but there are also shortcomings such as low strength and weak resistance to external damage. In order to change this shortcoming of silk, many scientific researchers have done a lot of research to strengthen silk. Through Raman spectroscopy and electron microscopy Imaging can show that the silk crystal structure after reinforcement is arranged more orderly, and various performances are significantly improved, but it is difficult to judge whether it involves changes in the amino acid sequence of silk protein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: A method based on proteomics analysis of carbon nanotube reinforced silk affecting the amino acid sequence of silk protein, the method adopts the following steps:

[0028] a) Select a portion of silkworm eggs from the same mother and incubate them under the same conditions. After the silkworms break out of their shells and grow up, they are randomly divided into two groups of A and B in equal amounts;

[0029] b) Spray 0.5 ml of an aqueous solution containing carbon nanotubes on the mulberry leaves eaten by the silkworm larvae of group A. The mulberry leaves eaten by the silkworm larvae of group B are not treated, and other growth conditions remain the same;

[0030] c) After the larvae spin and cocoon, two groups of silk are collected in 0.5% Na with a bath ratio of 1:100. 2 CO 3 The aqueous solution and the SDS mixed solution with a mass fraction of 0.5% were boiled for 30 minutes for degumming, and the degumming was performed twice. After 1 hour, the s...

Embodiment 2

[0035] Embodiment 2: a kind of method based on proteomics analysis carbon nanotube reinforced silk affects the amino acid sequence of silk protein, the method adopts the following steps:

[0036] a) Select a portion of silkworm eggs from the same mother and incubate them under the same conditions. After the silkworms break out of their shells and grow up, they are randomly divided into two groups of A and B in equal amounts;

[0037] b) Spray 1.5 ml of an aqueous solution containing carbon nanotubes on the mulberry leaves eaten by the silkworm larvae of group A, and the mulberry leaves eaten by the silkworm larvae of group B are not treated, and other growth conditions remain the same;

[0038] c) After the larvae spin and cocoon, two groups of silk are collected in 0.5% Na with a bath ratio of 1:100. 2 CO 3 The aqueous solution and the SDS mixed solution with a mass fraction of 1% were boiled for 30 minutes for degumming, and the degumming was performed twice. After 1 hour, ...

Embodiment 3

[0042] Embodiment 3: a kind of method based on proteomics analysis carbon nanotube reinforced silk affects the amino acid sequence of silk protein, the method adopts the following steps:

[0043] a) Select a portion of silkworm eggs from the same mother and incubate them under the same conditions. After the silkworms break out of their shells and grow up, they are randomly divided into two groups of A and B in equal amounts;

[0044] b) Spray 2.5 ml of an aqueous solution containing carbon nanotubes on the mulberry leaves eaten by the silkworm larvae of group A, and the mulberry leaves eaten by the silkworm larvae of group B are not treated, and other growth conditions remain the same;

[0045] c) After the larvae spin and cocoon, two groups of silk are collected in 0.5% Na with a bath ratio of 1:100. 2 CO 3 The aqueous solution and the SDS mixed solution with a mass fraction of 1.5% were boiled for 30 minutes for degumming, and the degumming was performed twice. After 1 hour...

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Abstract

The invention discloses a method for analyzing influence of carbon-nanotube-reinforced silk on fibroin amino acid sequences based on proteomics. The method has the advantages that mulberry leaves sprayed with a carbon-nanotube-containing solution are supplied to larva silkworms to obtain reinforced silk, a contrast experiment involving the reinforced silk and common silk according to a proteomics method is conducted, alkaline degumming is employed and SDS anionic surfactants are added for improving degumming effects in an experiment process, and chymotrypsin is used for enzyme digestion in the solution to complete enzyme digestion separation; HPLC-MS / MS is used for mass spectrometric analysis, and the influence of the carbon-nanotube-reinforced silk on the fibroin amino acid sequences is judged according to obtained two groups of amino acid sequences; the method is high in sensitivity and accuracy, simple to operate and less in sample consumption.

Description

technical field [0001] The invention relates to a method for influencing the amino acid sequence of silk protein, and more specifically relates to a method for analyzing the influence of carbon nanotube reinforced silk on the amino acid sequence of silk protein based on proteomics. Background technique [0002] Silk is one of the products of ancient Han civilization. It is a continuous long fiber that is solidified by secreting silk liquid when cooked silkworms make cocoons. It is also called "natural silk". It is one of the earliest animal fibers used by humans like wool. Silk has light , thin, soft and other advantages, but there are also shortcomings such as low strength and weak resistance to external damage. In order to change this shortcoming of silk, many scientific researchers have done a lot of research to strengthen silk. Through Raman spectroscopy and electron microscopy Imaging can show that the silk crystal structure after reinforcement is arranged more orderly,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8818G01N2030/8831
Inventor 彭志勤古锦翠王秉万军民胡智文
Owner ZHEJIANG SCI-TECH UNIV
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