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Method for cultivating fine hemp through stem tip

A technology of hemp and stem tip, which is applied in the field of hemp planting, can solve the problems of inability to produce fine hemp varieties on a large scale, and achieve the effects of maintaining excellent performance and active ingredient content, low incidence of diseases and insect pests, and robust plant growth

Active Publication Date: 2018-12-28
钦州市钦南区金窝工业园管理委员会
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a method for cultivating fine-seed hemp through the stem tip, which can solve the problem that the method of seed propagation of hemp cannot produce fine-seed hemp on a large scale

Method used

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Experimental program
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Embodiment Construction

[0016] Below in conjunction with specific embodiment the present invention is described in further detail:

[0017] This method for cultivating fine hemp by stem tip comprises the following steps:

[0018] A, plant screening: when the hemp is mature or when it germinates, select plants without damage by diseases and insect pests;

[0019] B. Pretreatment and disinfection: take the complete seed buds of the plant obtained in step A with a length greater than 3cm, clean them with clean water, rinse them with 70% alcohol for 30s, and then use 0.1% HgCl 2 Sterilize the solution for 3 minutes, wash with sterile water for 3 times or more;

[0020] C. Place the seed buds obtained in step B under a microscope, soak them in alcohol, and then use burnt and cooled tweezers and scissors to cut out the shoot tips with 1-2 leaf primordia, and then wash them with sterile water for 3 times or more ;

[0021] D. Cultivation: or put the stem tip segment obtained in step C into the induction ...

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Abstract

The invention discloses a method for breeding an improved variety of hemp through a shoot tip, belonging to the technical field of hemp plantation. The method comprises the following steps: plant screening, pretreatment and sterilization of cut embryo buds, cutting of leaf primordial, cultivation of tissue culture seedlings, domestication of the seedlings, transplanting of the seedlings, etc. The method provided by the invention overcomes the problem of great difficulty in large-scale production of hemp caused by low seed germination rate, long germination acceleration time, inconsistent germination time, difficulty in collection of hemp seed resources, incapability of remaining all the excellent properties of male parents and female parents due to variation of filial generations and the like in traditional seed propagation of hemp.

Description

technical field [0001] The invention relates to the technical field of hemp planting, in particular to a method for cultivating fine hemp varieties through stem tips. Background technique [0002] Hemp seedling propagation technology is mainly based on seed propagation, that is, sexual propagation method. The advantage of seed propagation is that a large number of seedlings can be obtained by one sowing, and the collection, storage and transportation of seeds are convenient. The seedlings grow vigorously, have strong resistance to stress, and are easy to domesticate . But traditional hemp seed propagation has the following defects: (1) the seed germination rate is low, the highest only reaches about 80%; (2) the germination time reaches about 20 days, and the germination time is inconsistent; (3) hemp resources are in short supply, It is difficult to collect in batches; (4) The possibility of sexual reproduction is high, and the offspring may not be able to retain all the e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 罗永志
Owner 钦州市钦南区金窝工业园管理委员会