Method for improving content of mogroside III

A technology of mogroside and Luo Han Guo, which is applied in horticultural methods, botanical equipment and methods, chemicals for biological control, etc., can solve the problems of restricting the healthy development of the Luo Han Guo industry and low content, and achieve strong practicability and promotion value , rich growth environment, easy operation

Inactive Publication Date: 2017-05-10
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have found that glucoside V is transformed from low glycosides such as glycoside III, especially in the later stage of fruit development, glucoside III and other low glycosides are completely consumed and converted into sweet glycoside V, however, glucoside II

Method used

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  • Method for improving content of mogroside III

Examples

Experimental program
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Embodiment 1

[0034] A method for increasing the content of mogroside Ⅲ. The tissue cultured seedlings of Luo Han Guo were inoculated in a solid medium containing methyl jasmonate for tissue culture for 28 days, and methyl jasmonate was applied to the tissue cultured seedlings of Luo Han Guo to induce the metabolism of mogroside Ⅳ. The high expression of enzymes (such as glucosyltransferase and cytochrome P450 enzymes, etc.) genes can rapidly increase the content of mogroside III in a short period of time, and the concentration of methyl jasmonate is 400 μmol / L. Afterwards, the conventional cultivation method of Luo Han Guo is used to cultivate until the fruit of Luo Han Guo is formed. In this embodiment, the formulation of the basal medium in the solid medium is: MS, 1.5mg / L6-BA, 0.3mg / L IBA, 3.5g / L agar, 30g / L sucrose and 1.0g / L activated carbon. Before solidification of the solid medium, methyl jasmonate was added to make the concentration 400 μmol / L.

Embodiment 2

[0036] A method for increasing the content of mogroside III,

[0037]Step 1 Tissue culture, inoculate the tissue cultured seedlings of Luo Han Guo in solid medium containing methyl jasmonate for tissue culture for 35 days, the culture conditions are relative humidity 60-66%, light intensity 1400lux, light time 8h / d, temperature 23 ±2°C, wherein the solid medium includes: basal medium (MS, 1.5mg / L 6-BA, 0.3mg / L IBA, 3.5g / L agar, 30g / L sucrose, 1.0g / L activated carbon) and the methyl jasmonate of 220 μmol / L; the preparation method of the solid medium is as follows: the methyl jasmonate is dissolved in the ethanol aqueous solution that volume fraction is 2%, is mixed with the methyl jasmonate mother liquor of 50mmol / L, and with 0.22 μm The microporous filter membrane is sterilized, and the sterilized methyl jasmonate mother liquor is added to the basal medium before solidification, so that the concentration of methyl jasmonate is 220 μmol / L, sterilized and cooled to 26 ° C, which...

Embodiment 3

[0040] On the basis of Example 2, before transplanting after tissue culture, scratch 1-2 0.8mm deep wounds with the tissue cultured seedlings of Luo Han Guo apart from the root 1cm above, and will contain 0.03mg / L bee venom and 0.2mg / L The inducer of L calcium nitrate (the solvent is water) is drip-coated on the wound, and each plant of Luo Han Guo tissue culture seedling is drip-coated with 0.05mL, and then the aloe vera gel is coated on the wound to form a film with a thickness of 1-1.5mm, and then apply The tissue-cultured seedlings of Luo Han Guo were irradiated for 0.5 h under medium-wave ultraviolet rays with a wavelength of 280-315 nm and then used for transplanting.

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Abstract

The invention discloses a method for improving the content of mogroside III. The method comprises the following steps: inoculating siraitia grosvenorii tissue culture seedlings into a solid culture medium containing methyl jasmonate and carrying out tissue culture for 28 to 35 days, wherein the concentration of the methyl jasmonate is 50mu.mol/L to 400mu.mol/L. The method has the characteristics of rapid effect, low cost, simplicity in operation, convenience for implementation, no toxin and no residues of products and the like, so that large-scale production of the mogroside III is realized.

Description

technical field [0001] The invention relates to the technical field of mangosteen cultivation. More specifically, the present invention relates to a method for increasing the content of mogroside III. Background technique [0002] Luo Han Guo (Siraitia grosvenorii) is a precious medicinal and sweet plant unique to China. Its fruit is cool in nature and sweet in taste, and has the functions of clearing away heat and moistening lungs, relieving throat and opening sound, laxative and anticancer. The active ingredient glucoside V is one of the strongest non-sugar sweeteners in the world. It is 300-400 times higher than sugar, widely used in food, health products and medicines, and is an ideal sugar substitute for diabetics, obese people and hypertensive patients. Studies have found that glucoside V is transformed from low glycosides such as glycoside III, especially in the later stage of fruit development, glucoside III and other low glycosides are completely consumed and conv...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G7/06A01G9/10A01N37/42A01P21/00
CPCA01G7/06A01G9/0299A01H4/00A01H4/001A01N37/42
Inventor 韦荣昌
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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