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A culture device for suspended tissue cells

A culture device and technology for suspending tissue, which is applied in tissue cell/virus culture devices, general culture methods, 3D culture, etc., can solve problems such as unfavorable normal cell growth, cell damage, slowing down of cell growth, etc., to ensure smooth and normal The effect of growth, uniform distribution of cells, and convenience for normal growth

Inactive Publication Date: 2017-05-10
CHONGQING RUNZE PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When culturing cells with the above-mentioned prior art, along with the increase of cell density, it is difficult to achieve uniform distribution of cells, which will form a local cell-intensive area, affecting the transmission of the culture solution, and the concentration of the culture solution is uneven, which is not conducive to the normal growth of cells. The high cell density will also slow down the cell growth rate. The stirring method of the above-mentioned prior art has a large shear force, which will cause damage to the cells and is not conducive to the cultivation of the cells.

Method used

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  • A culture device for suspended tissue cells
  • A culture device for suspended tissue cells
  • A culture device for suspended tissue cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Referring to the accompanying drawings, a culture device for suspending tissue cells includes a tissue cell culture body 2, which is a porous tantalum material; the porous tantalum material is based on the material pore size The grading cavity and the cavity wall surrounding the cavity are formed, and the lower-level small cavity is set around the cavity wall forming the upper-level large cavity; the cavities of each level are connected to each other, and the cavities of each level are also connected to each other. . The culture device also includes a cyclone device placed therein.

[0045] Specifically in this example, the porous tantalum material has three-level cavities, wherein the upper two-level large cavities 12 (hereinafter referred to as the first-level cavities) that are uniformly distributed and interpenetrated have uniformly distributed, Interconnected upper-level large cavities 14 (hereinafter referred to as second-level cavities), and evenly distributed a...

Embodiment 2

[0059] The cell culture body 2 of the tissue cell culture device of this embodiment is made of porous silica material, has two-stage cavities, uniformly distributed and interpenetrated upper-level macro-cavities 12 have evenly distributed and inter-connected lower-level cavities on the cavity wall 13 small cavity 14, and the two-stage cavity also communicates with each other. The pore diameter of the upper large cavity 12 is 20µm-80µm, and the pore diameter of the lower small cavity 14 is 100nm-300nm. The included angles θ1 and θ2 of outlet pipe ① 11, outlet pipe ② 12 and the horizontal plane are 15°, outlet pipe ① 11, outlet pipe ② 12 are equipped with a perforated diaphragm 16, and its average aperture is 0.3mm.

[0060] The preparation method of this kind of porous silicon dioxide material is:

[0061] (1) Material preparation

[0062] Use 1µm-10µm silica powder and urea with a particle size of 130nm-350nm as the smallest pore-forming agent, mix them evenly, and use 130nm...

Embodiment 3

[0070] The cell culture body 2 of the tissue cell culture device of this embodiment is made of porous titanium material and has three-level cavities, among which, the cavities of the upper two-level large cavities 12 (hereinafter referred to as the first-level cavities) that are uniformly distributed and interpenetrating On the wall 13 there are evenly distributed and interconnected upper-level large cavities 14 (hereinafter referred to as second-level cavities), and on the wall 15 of the upper-level large cavities there are evenly distributed and inter-connected lower-level small cavities ( Hereinafter referred to as the third-level cavity); and the cavities of all levels are also connected to each other. The aperture of the first-stage cavity 12 is 300µm-700µm, the aperture of the second-stage cavity 14 is 60µm-95µm, and the aperture of the third-stage cavity is 400nm-700nm. Be 45 °, air outlet pipe 1. 11, outlet pipe 2. 12 outlets are equipped with apertured diaphragm 16, a...

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Abstract

The invention relates to a culture device for suspended tissue cells. The device includes a tissue cell culture body. The tissue cell culture body is a porous material. The porous material consists of pore cavities graded according to pore diameters and cavity walls forming the pore cavities in a surrounding manner. The cavity walls forming the upper-grade pore cavities in the surrounding manner are provided with lower-grade pore cavities. The pore cavities in each grade are communicated with each other, and the pore cavities of different grades are communicated with each other. The culture device also includes a cyclone apparatus disposed in the culture device. The culture device is particularly beneficial to free normal growth of suspended cells in a three-dimensional space. A cell over-dense zone or a nutrient enriched zone is not liable to be generated in a cell culturing process, cells are uniformly distributed and normal cell growth is facilitated.

Description

technical field [0001] The invention relates to a tissue cell culture device, in particular to a tissue cell culture device which is conducive to the free and normal growth of suspended cells in a culture solution under a culture environment. Background technique [0002] Cell culture refers to a culture technique in which cells are removed from tissues in the body, simulated in vivo environment, and grown and reproduced in vitro, and maintain their structure, function, and physiological characteristics under sterile, appropriate temperature, pH and certain nutritional conditions. . Cell culture can be divided into two categories: attached growth and suspension growth according to the characteristics of whether they can be attached to the support in the culture vessel. Adhesive cells can grow on the surface of the support during culture. Generally, fibers, microcarriers, porous materials, etc. can be used as culture media. Attached to the support to grow, when it is cultur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/02C12M1/04C12M1/22
CPCC12M21/08C12M23/10C12M29/06C12M25/14C12M27/04C12M3/02C12M3/04C12M23/08C12M23/16C12M25/18C12N5/0062C12N5/0068
Inventor 叶雷
Owner CHONGQING RUNZE PHARM CO LTD
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