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Bama miniature pig MC1R mutant gene causing albinsim and application thereof

A technology of Bama fragrant pig and mutant gene, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as unfavorable experimental observation

Inactive Publication Date: 2017-05-10
SHENZHEN JINXINNONG FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

White-coated animals are often used as ideal models in animal models of medical diseases. The middle part of the coat color of ordinary Bama pigs is white, and the two ends are black, which has disadvantages for experimental observation.

Method used

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  • Bama miniature pig MC1R mutant gene causing albinsim and application thereof
  • Bama miniature pig MC1R mutant gene causing albinsim and application thereof
  • Bama miniature pig MC1R mutant gene causing albinsim and application thereof

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Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1: The establishment of albino Bamaxiang pig genetic family

[0026] Taking my country's Bama pig as a model animal, a genetic family of albino Bama pig with highly stable recessive inheritance was accidentally obtained during the process of inbreeding. The specific process is as follows:

[0027] The first stage: the F2 population was obtained by mating an F1 established boar with wild-type Bamaxiang pigs, and the F2 sows were selected to backcross with the F1 boars to generate the F3 generation; albino individuals were accidentally found in the F3 generation, for detection For its heritability, increase the backcross of F2 sows and F1 boars to produce a sufficient number of F3 generations. As a result, a total of 125 F3 generations were born, including 29 albino individuals and 96 normal individuals;

[0028] The second stage (in progress): use the F3 generation albino Bama pig boar to cross with the F3 generation albino Bama pig sow to produce the F4 gene...

Embodiment 2

[0038] Embodiment 2: analysis of family characteristics

[0039] The appearance and color characteristics of normal Bamaxiang pigs are mainly black at the two ends and white in the middle (such as figure 1 As shown), some individuals have black spots on the back and waist, and white lines or inverted triangle-shaped white spots on the forehead, commonly known as "two-headed black" and "plantain pig". The appearance and color characteristics of the mutant Bamaxiang pig are covered with white hair (such as figure 2 shown).

Embodiment 3

[0040] Embodiment 3: family gene mapping

[0041] Numerous documents have shown that the melanocortin 1 receptor 1 (Melanocortin 1 Receptor, MC1R) gene plays an important role in the process of controlling animal melanin synthesis, and has been confirmed as the main gene controlling animal coat color. Therefore, in this study, the exons of the MC1R candidate genes in this albino Bamaxiang pig genetic family were sequenced, and a total of 58 individuals in the family were detected, including 29 albino individuals, 25 normal individuals, and 4 phenotypically normal individuals carrying recessive Genetic individuals, sequencing found: 29 albino individuals had two CCs inserted at the 894th base of the MC1R gene, and they were homozygous mutations, named -- / --genotype; 4 individuals with normal phenotypes carried recessive inheritance Gene individuals also found two CC insertions at the 894th base of the MC1R gene, but they were heterozygous mutations, named -- / CC genotype; 25 nor...

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Abstract

The invention discloses a Bama miniature pig MC1R mutant gene causing albinsim and application thereof. The Bama miniature pig MC1R mutant gene is formed by inserting two CC basic groups behind the 894th position of an MC1R gene. The mutation can cause albinsim of the Bama miniature pigs and represent recessive inheritance. The mutant gene can be used for breeding the strain of albinsim Bama miniature pigs, and the albinsim Bama miniature pigs completely covered with purely white hair on the body surface is preferably used as a medical experiment animal model in comparison with conventional strain of Bama miniature pigs having white hair between front and rear parts of the body. Furthermore, the Bama miniature pig serving as a main pet pig, and breeding of albinsim strain can meet preference of partial pet pig lovers for completely white hair. The complete white hair can be used as a genetic marker and applied to evaluation of purity, genetic stability and genetic relationship of the variety and determination of the type of hybrid combination of parent in commercial strain breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the MC1R mutation gene of Bamaxiang pigs which causes albinism, and also relates to the application of the gene. Background technique [0002] The pigment that affects pig coat color is mainly melanin, which can be divided into eumelanin and pheomelanin. The content and distribution of eu / pheomelanin in the coat of different breeds of pigs resulted in different coat color types. Melanocortin 1 Receptor (MC1R) gene plays an important role in the process of controlling animal melanin synthesis, and has been confirmed as the main gene controlling animal coat color. Kijas J M et al. A CC insertion mutation was found at positions 67-68 of the MC1R coding region in Tamworth and Hereford pig breeds. This frameshift mutation may cause recessive red coat color. Dun Guiling et al. (Dun Guiling, Li Xianglong, Cao Hongzhan, et al. Study on gene variation of melanocortin receptor 1...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12Q1/68A01K67/027
CPCC07K14/72A01K67/027A01K2217/07A01K2227/108A01K2267/03C12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 魏泓商海涛张仕斌文乐常凤琴
Owner SHENZHEN JINXINNONG FEED
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