Unlock instant, AI-driven research and patent intelligence for your innovation.

Deglycated sea cucumber secondary saponins and preparation method thereof

A secondary saponin and sea cucumber saponin technology, which is applied in the field of sea cucumber saponin enzymatic hydrolysis preparation, can solve the problems of less sea cucumber saponin enzymatic hydrolysis, poor specificity, and many side reactions, and achieve the goal of improving biological activity, reducing toxicity, and single product Effect

Active Publication Date: 2021-07-27
OCEAN UNIV OF CHINA
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there are very few related studies on the enzymatic hydrolysis of sea cucumber saponins, so the enzymatic hydrolysis method in Gao Juan's paper has very good reference and guiding significance for the study of the enzymatic hydrolysis of sea cucumber saponins by glycosidases
Common chemical methods are used to hydrolyze saponins, but they have the disadvantages of many side reactions, miscellaneous products, and poor specificity.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Deglycated sea cucumber secondary saponins and preparation method thereof
  • Deglycated sea cucumber secondary saponins and preparation method thereof
  • Deglycated sea cucumber secondary saponins and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Put the purchased dried sea cucumber into an oven at 50°C, take it out after 2 days, and crush it into powder;

[0042] (2) Take dried sea cucumber powder (Philippine sea cucumber about 1 kg), extract it with 60% ethanol for 7 days, stir continuously during this period, extract 3 times, evaporate under reduced pressure to obtain extract paste, and freeze-dry to obtain crude extract;

[0043] (3) Dissolve the above crude extract fully with a small amount of water first, and then extract five times with water-saturated n-butanol. The first time extracts the sample and water-saturated n-butanol 1:3 (v / v), and then extracts it twice with water-saturated n-butanol. 1:2 (v / v) extraction, the last two extractions were 1:1 (v / v). Concentrate the supernatant under reduced pressure to obtain extract extract, and evaporate to dryness in a 60°C water bath to obtain crude saponin powder;

[0044] ⑷, enrich the thick saponin in step ⑶ with macroporous resin column chromatography...

Embodiment 2

[0053] (1) Put the purchased dried sea cucumber into an oven at 50°C, take it out after 2 days, and crush it into powder;

[0054] (2) Take dried sea cucumber powder (Philippine sea cucumber about 1kg), extract it with 60% ethanol for 7 days, keep stirring during the period, extract 3 times, evaporate under reduced pressure to obtain extract paste, freeze dry in a freeze dryer to obtain crude extract ;

[0055] (3) Dissolve the above crude extract fully with a small amount of water first, and then extract five times with water-saturated n-butanol. The first time extracts the sample and water-saturated n-butanol 1:3 (v / v), and then extracts it twice with water-saturated n-butanol. 1:2 (v / v) extraction, the last two extractions were 1:1 (v / v). Concentrate the supernatant under reduced pressure to obtain extract extract, and evaporate to dryness in a 60°C water bath to obtain crude saponin powder;

[0056] ⑷, enrich the thick saponin in the step ⑶ with macroporous resin column ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides deglycated sea cucumber secondary saponins and a preparation method thereof. The deglycated sea cucumber secondary saponins are obtained after the sugar chains of sea cucumber saponin HA and sea cucumber EA are removed by glycosidase enzymatic hydrolysis. Grade saponin; the preparation method of this secondary saponin includes: ethanol leaching, macroporous resin rough extraction, and then using silica gel column to purify to obtain HA and EA respectively, and finally using glycosidase to carry out enzymatic hydrolysis, that is, the deglycationized HA and EA secondary saponins. The glycosidase used in the present invention enzymatically hydrolyzes sea cucumber saponin into secondary saponin under mild conditions, thereby reducing the toxicity of sea cucumber saponin and improving its biological activity; at the same time, using glycosidase to enzymolyze sea cucumber saponin avoids the use of microbial degradation or The side reactions and by-products brought about by the degradation of crude enzymes extracted from microorganisms improve the purity and yield of enzymatic hydrolysis products, and a single product can be obtained with a purity of up to 100%.

Description

technical field [0001] The invention relates to an enzymatic hydrolysis preparation method of sea cucumber saponins, in particular to a method for enzymatic hydrolysis of sea cucumber saponins with glycosidase, and finally obtains sea cucumber secondary saponins from which two sugar groups have been removed. Background technique [0002] Sea cucumbers belong to the phylum Echinodermata, the class Holothuria, and the order Chiroptera. Sea cucumber saponins are important secondary metabolites and active substances in sea cucumbers. The main active functions of sea cucumber saponins are anti-tumor, anti-fungal, anti-virus, anti-aging and hemolysis, as well as regulating the growth and development of specific cells and immunity. adjustment etc. Therefore, sea cucumber saponins are an important indicator of the medicinal value of sea cucumbers. [0003] Saponins have a hemolytic effect, and saponins can combine with red blood cell membranes to form sterol complexes, leading to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20
CPCC12P33/20
Inventor 李兆杰陈李品陈静兰王静凤王玉明薛勇薛长湖
Owner OCEAN UNIV OF CHINA