Bi-directional scanning imaging method based on TDI-CCD (time delay integration-charge coupled device) in fluorescent optical micro-imaging

Abstract

The invention provides a bi-directional scanning imaging method based on a TDI-CCD (time delay integration-charge coupled device) in fluorescent optical micro-imaging. The imaging method includes the steps: S1 setting triggering window information; S2 applying first electric level control signals to the TDI-CCD, and enabling the TDI-CCD to scan forwards; S3 controlling a three-dimensional precise movement control platform to move forwards along an X-axis according to the triggering window information, and giving triggering signals to control the TDI-CCD to realize exposure imaging along the forward scanning direction according to the moving position of the three-dimensional precise movement control platform; S4 applying second electric level control signals to the TDI-CCD, and enabling the TDI-CCD to scan backwards; S5 controlling the three-dimensional precise movement control platform to move backwards along the X-axis according to the triggering window information, and giving triggering signals to control the TDI-CCD to realize exposure imaging along the backward scanning direction according to the moving position of the three-dimensional precise movement control platform. The direction of the first electric level control signals is contrary to that of the second electric level control signals. The method can effectively detect fluorescent weak signals, integral imaging can be accelerated, and whole fluorescent optical imaging period is shortened.

Description

technical field [0001] The invention belongs to the technical field of fluorescent optical microscopic imaging, and more specifically relates to a TDI-CCD-based bidirectional scanning imaging method in fluorescent optical microscopic imaging. Background technique [0002] TDI (Time Delay Integration) CCD is a time-delay integration CCD is a linear scanning imaging device, which is developed based on the concept of multiple exposures to the same object, and is usually used to image some high-speed objects. Now industrial monitoring, space exploration, aerospace remote sensing and other fields have a wide range of applications. [0003] At present, TDI-CCD has also developed to a certain extent in the field of fluorescence optical imaging, especially for detecting weak fluorescence signals. Motion synchronization, as the image moves from one row of pixels to another row, the integral charge also moves with it, in this way, the continuous imaging output of moving objects provi...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the defects of the prior art, the present invention proposes a two-way scanning method based on TDI-CCD to realize faster imaging of biological tissue samples, aiming to solve the problem of long imaging cycle and low efficiency of biological tissue samples using fluorescence microscopic imaging

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