Method for determining components of common wedgelet fern herb by adopting quantitative analysis multi-components by single marker
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A technology of goldenrod and orientin, which is applied in the field of determining the components of goldenrod by using the method of one test and multiple evaluation, so as to achieve the effect of cost saving, fast method, time saving and cost saving
Active Publication Date: 2017-05-10
GUANGXI UNIV OF CHINESE MEDICINE
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[0022] The purpose of the present invention is to provide a method for determining the composition of Golden Flower Grass by using the method of "one tes
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[0068] Using the method of "one test and multiple evaluation" to determine the components of Golden Flower Grass, the cheap and easy-to-obtain protocatechuic acid was used as an internal reference to conduct one test and multiple evaluation, and to establish the relationship between protocatechuic acid and protocatechuic acid, protocatechuic acid in Golden Flower Grass. Relative correction factors among tea aldehyde, orientin and vitexin, by which the content of protocatechuic acid, protocatechualdehyde, orientin and vitexin in Rhizoma macrophylla was calculated and determined by liquid chromatography , the specific method is as follows:
[0069] (1) Material preparation: the test sample was collected from Guangxi, and it was identified as the whole herb of Stenoloma chusanaum Ching, a plant of the family Stenoloma chusanaum Ching, by the Medicinal Botanical Garden of Guangxi University of Traditional Chinese Medicine; the test product was ground into powder , passed through N...
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Abstract
The invention provides a method for determining components of common wedgelet fern herb by adopting quantitative analysis multi-components by a single marker. The method is characterized in that the quantitative analysis multi-components by the single marker is carried out by taking protocatechuic acid which is cheap and easy to obtain as an internal reference object, and relative correction factors between protocatechuic acid, and protocatechuic acid, protocatechuic aldehyde, orientoside and vitexin in common wedgelet fern herb are established; the content of protocatechuic acid, protocatechuic aldehyde, orientoside and vitexin in common wedgelet fern herb is calculated through the correction factors; and a liquid chromatography is used for determining. The detection method is high in practicability and simple to operate and the cost is saved; and the content of four components including protocatechuic acid, protocatechuic aldehyde, orientoside and vitexin in common wedgelet fern herb can be effectively detected.
Description
technical field [0001] The invention relates to a quality detection method, in particular to a method for measuring the composition of the golden flower grass by adopting a method of one test and multiple evaluations. Background technique [0002] Daye Jinhuacao comes from "Guangxi Traditional Chinese Medicine Records". [0003] Perennial herb, up to 65 cm high. The rhizome is hard and short, running horizontally, densely covered with reddish brown subulate scales. Leaves close, petiole up to 25 cm, stalk color, bright, erect; leaves nearly leathery, glabrous; 3-4 times pinnately divided, lanceolate, 20-40 cm long, 5-12 cm wide; lower feathers Sheets ovate or needle-shaped, obliquely spread, 5-10 cm long, 2-5 cm wide; pinnae oblong or lanceolate; final lobes cuneate, apex truncate, with teeth, base cuneate, decurrent, Veins obvious below, 2 forked branches. The sporangium is terminal, with 1-2 pieces on each lobe, the cover of the sporangia is grayish brown, half cup-sha...
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