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Preparation method of porcine bone protein

A technology of active peptides and pig bones, applied in the biological field, can solve the problems of easy corruption and deterioration of bones, lower bone utilization rate, waste, etc., and achieve the effect of improving the degree of hydrolysis and anti-oxidation ability

Inactive Publication Date: 2017-05-17
曹书华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In pig bones, the utilization of bone protein, a high-quality nutrient source, has been lagging behind, and the protein and other nutrients in the bone have not been fully developed and utilized.
At present, because animal bones are cheap and difficult to store, they are often processed into products with relatively low added value or made into feed, which greatly reduces the utilization rate of bones
This will not only cause great waste, but also cause certain environmental pollution because the bones are extremely easy to rot and deteriorate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Remove the fresh pork bone and roll it into 1cm 3 About block, rinse with hot water at 60°C to remove grease, then soak in 1% citric acid solution for 5 hours; the dosage ratio of pork bone to citric acid solution is 1kg: 2L;

[0038] (2) Take out and drain, crush and grind into bone paste, add in 2% vitamin C aqueous solution by mass fraction, soak for 6 hours, and obtain the enzymatic hydrolysis substrate; the dosage ratio of pig bone and vitamin C aqueous solution is 1kg: 2L;

[0039] (3) adding 200,000 U / g of acid protease at a mass ratio of 1%, and enzymatically hydrolyzing it for 2 hours at 50°C;

[0040] (4) Enzyme inactivation: after enzymatic hydrolysis, heat up to inactivate the enzyme;

[0041] (5) Filtration: adding perlite, diatomaceous earth and other filter aids, plate and frame filtration to obtain the hydrolyzate;

[0042] (6) Concentration: the hydrolyzate is concentrated by a scraper concentrator to obtain an extract with a mass fraction of 30%;...

Embodiment 2

[0046] (1) Remove the fresh pork bone and roll it into 1cm 3 About block, rinse with hot water at 60°C to remove grease, then soak in 1% citric acid solution for 5 hours; the dosage ratio of pork bone to citric acid solution is 1kg: 2L;

[0047] (2) Take out and drain, crush and grind into bone paste, add to vitamin C aqueous solution with a mass fraction of 2%, soak for 6 hours to obtain the enzymatic hydrolysis substrate; the dosage ratio of pig bone and vitamin C aqueous solution is 1kg: 2L;

[0048] (3) adding 200,000 U / g of acid protease at a mass ratio of 1%, and enzymatically hydrolyzing it for 3 hours at 45-50°C;

[0049] (4) Enzyme inactivation: after enzymatic hydrolysis, heat up to inactivate the enzyme;

[0050] (5) Filtration: add filter aids such as perlite and diatomaceous earth, and plate and frame filter to obtain hydrolyzate;

[0051] (6) Concentration: the hydrolyzate is concentrated by a scraper concentrator to obtain an extract with a mass fraction of 30...

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Abstract

The invention discloses a preparation method of porcine bone protein, and belongs to the technical field of biology. The preparation method has the advantages that porcine bone is pretreated through citric acid solution soaking and vitamin C solution soaking; compounding is performed; two kinds of enzymes are used for performing compound enzymatic hydrolysis; the hydrolysis degree and the antioxidation capability of the porcine bone protein are obviously improved.

Description

technical field [0001] The invention relates to a preparation method of porcine bone active peptide, which belongs to the field of biotechnology. Background technique [0002] In pig bones, the utilization of bone protein, a high-quality nutrient source, has been lagging behind, and the protein and other nutrients in the bone have not been fully developed and utilized. At present, because animal bones are cheap and difficult to store, they are often processed into products with relatively low added value or made into feed, which greatly reduces the utilization rate of bones. This will not only cause great waste, but also cause certain environmental pollution because the bones are very easy to rot. [0003] There are two main forms of bone comprehensive utilization, one is whole bone utilization, that is, the utilization of the whole bone, this method can more comprehensively utilize the nutrients in the bone, and its main products are bone meal, bone paste, etc., which can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A23L33/18A23K20/147A61K38/01A61P39/06
CPCC12P21/06A61K38/012C07K1/34
Inventor 曹书华
Owner 曹书华
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