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Biological fluorescence detection system and detection method thereof

A biological detection system and fluorescence detection technology, applied in the direction of fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve the problems of slow analysis speed and high detection cost, and achieve low cost, high sensitivity, and short measurement time Effect

Active Publication Date: 2017-05-24
深圳沃德生命科技有限公司
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Problems solved by technology

[0006] In view of the above deficiencies in the prior art, the purpose of the present invention is to provide a fluorescent biodetection system and its detection method, aiming at solving the problem of slow analysis speed and high detection cost in the prior art of performing the same analysis on multiple samples. question

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  • Biological fluorescence detection system and detection method thereof
  • Biological fluorescence detection system and detection method thereof

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Embodiment Construction

[0030] The present invention provides a fluorescent biological detection system and a detection method thereof. In order to make the purpose, technical solution and effect of the present invention clearer and clearer, the present invention is further described in detail below. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

[0031] The present invention provides a preferred embodiment of a fluorescent biological detection system, which includes an incubator, a pipeline connected to one end of the incubator, a fluid power unit is arranged in the pipeline, and a magnetic component (such as a soft magnet) is arranged below the pipeline;

[0032] The incubator is used to generate magnetic microparticles connected with phycoerythrin;

[0033] The fluid power unit is used to drive the generated magnetic micro-particles to flow along the pipeline;

[0034] The magnetic ass...

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Abstract

The invention discloses a biological fluorescence detection system and a detection method thereof. Antibody coated magnetic microparticles, biotinylated detection antibody, a sample to be analyzed and streptavidin phycoerythrin are added to an incubator for joint incubation, magnetic microparticles connected with the phycoerythrin are generated, and the magnetic microparticles flow along a pipeline. When the magnetic microparticles flow through the upper portion of a magnetic component, the magnetic component gathers all the magnetic microparticles together, the gathered magnetic microparticles are irradiated by using a light source, the phycoerythrin connected to the magnetic microparticles is stimulated to emit out fluorescent light, the produced fluorescent light is collected for measurement, and the information of analyte concentration in an analysis sample can be obtained. All the magnetic microparticles are gathered together for measurement, the measurement time is short, the sample analysis speed is improved. The used magnetic microparticles are not needed to be encoded, the cost is low, and accordingly the detection sensitivity is improved by adopting a biotin and avidin bonding method.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a fluorescent biological detection system and a detection method thereof. Background technique [0002] The construction of highly sensitive analytical methods has always been one of the goals pursued in the field of analytical chemistry. Highly sensitive analytical methods have promoted the development of analytical chemistry and its applications in clinical diagnosis, environmental monitoring, food safety, bioimaging and other fields. Highly sensitive analytical methods are especially important in the early diagnosis of major infectious diseases, bacterial or viral infections, cancer and other diseases, and the screening of trace toxic substances in food and the environment. [0003] At present, the analysis methods mainly include enzyme-linked immunoassay, colloidal gold immunochromatographic test strip, chemiluminescence immunoassay, and radioimmunoassay. Colloidal gold i...

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Application Information

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IPC IPC(8): G01N33/543G01N21/64
CPCG01N21/6428G01N21/645G01N33/54326
Inventor 金莹
Owner 深圳沃德生命科技有限公司
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