Preparation method of a novel pesticide bovine serum albumin-thiacloprid nanoparticle

A bovine serum albumin and nanoparticle technology, which is applied in the field of preparation of new pesticide bovine serum albumin-thiacloprid nanoparticles, can solve the problems of difficult long-term storage, difficult transportation, obvious burst release phenomenon, etc., and achieves low equipment requirements. , the process is simple, the effect of reducing the manufacturing cost

Active Publication Date: 2022-03-18
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there are various thiacloprid dosage forms on the market, such as powder, wettable powder, granule, water suspension, microcapsule powder, microcapsule water suspension, etc., all of which have certain transportation difficulties and obvious burst release phenomenon. Not easy to store for a long time and many other problems

Method used

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  • Preparation method of a novel pesticide bovine serum albumin-thiacloprid nanoparticle

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Take 0.08g of bovine serum albumin in 4ml of double-distilled water to prepare an aqueous phase, 50 o C water bath for 30min to obtain clear and transparent bovine serum albumin aqueous solution;

[0026] (2) Take another 1 mg of thiacloprid, add 0.167 ml of acetonitrile to dissolve by ultrasonic for 5 minutes, and prepare 6 mg / mL of thiacloprid-acetonitrile oil phase;

[0027] (3) Adjust the rotational speed of the water phase to 1000rpm, add the thiacloprid-acetonitrile oil phase dropwise under high-speed stirring, emulsify for 30min, and take out the emulsion quickly at 0-4. o Cool down for 10min at C, then take out the ultrasound for 10min to obtain bovine serum albumin-thiacloprid ultramicroemulsion;

[0028] (4) Dialyze the ultra-microemulsion dialysis membrane (MWCO=5000) overnight, take out the dialysate, freeze, and freeze-dry to obtain white powder, namely thiacloprid albumin nanoparticles.

Embodiment 2

[0030] (1) Take 0.08g of bovine serum albumin in 4ml of double-distilled water to prepare an aqueous phase, 50 o C water bath for 30min to obtain clear and transparent bovine serum albumin aqueous solution;

[0031] (2) Take another 2 mg of thiacloprid, add 0.334 ml of acetonitrile to dissolve it by ultrasonic for 5 minutes, and prepare 6 mg / mL of thiacloprid-acetonitrile oil phase;

[0032] (3) Adjust the rotation speed of the water phase to 1000rpm, add the thiacloprid-acetonitrile oil phase dropwise under high-speed stirring, emulsify for 40min, and take out the emulsion quickly at 0-4. o Cool down for 10min at C, then take out the ultrasound for 10min to obtain bovine serum albumin-thiacloprid ultramicroemulsion;

[0033] (4) Dialyze the ultra-microemulsion dialysis membrane (MWCO=5000) overnight, take out the dialysate, freeze, and freeze-dry to obtain white powder, namely thiacloprid albumin nanoparticles.

Embodiment 3

[0035] (1) Take 0.08g of bovine serum albumin in 4ml of double-distilled water to prepare an aqueous phase, 50 o C water bath for 30min to obtain clear and transparent bovine serum albumin aqueous solution;

[0036] (2) Take another 3 mg of thiacloprid, add 0.501 ml of acetonitrile to dissolve it by ultrasonic for 10 minutes, and prepare 6 mg / mL of thiacloprid-acetonitrile oil phase;

[0037] (3) Adjust the rotational speed of the water phase to 1200rpm, add the thiacloprid-acetonitrile oil phase dropwise under high-speed stirring, emulsify for 40min, and take out the emulsion quickly at 0-4. o Cool down for 5min at C, then take out ultrasonic wave for 10min to obtain bovine serum albumin-thiacloprid ultramicroemulsion;

[0038] (4) Dialyze the ultra-microemulsion dialysis membrane (MWCO=5000) overnight, take out the dialysate, freeze, and freeze-dry to obtain white powder, namely thiacloprid albumin nanoparticles.

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Abstract

The invention discloses a method for preparing bovine serum albumin-coated thiacloprid novel pesticide nanoparticles. The process of wrapping thiacloprid with bovine serum albumin adopts the emulsification and solidification method. First, a certain amount of bovine serum albumin is dissolved in double distilled water to prepare the water phase, and thiacloprid is dissolved in acetonitrile to make the oil phase. , adding the oil phase into the water phase drop by drop, emulsifying for a period of time, cooling rapidly, ultrasonically pulverizing to obtain a microemulsion, freezing, and lyophilizing to obtain thiacloprid albumin nanoparticles. The new albumin nano-preparation technology adopted in the method is to load the drug into the albumin without changing the structure of the albumin, so as to increase the drug load and have the function of sustained release. The use of nanotechnology can miniaturize pesticide products, reduce resource waste, reduce costs, significantly improve drug efficacy, effectively increase the contact area between pesticides and target pests, make them easier to be swallowed and absorbed, and better exert contact and stomach poisoning effects. Realize the high efficiency, low toxicity and safety of nano biological pesticides.

Description

technical field [0001] The invention discloses a preparation method of a novel pesticide bovine serum albumin-thiacloprid nanoparticle, in particular, the process of encapsulating thiacloprid with bovine serum albumin adopts an emulsification solidification method. It belongs to the technical field of nanomaterials and new pesticides. Background technique [0002] Thiacloprid is another new chloronicotinoid insecticide developed by Bayer after imidacloprid with the chemical name (3-((6-chloro-3-pyridyl)methyl)-1,3-thiazole Lin-2-ylidene) cyanamide has high efficiency, low toxicity, broad-spectrum effect, and also has stomach toxicity, contact killing and systemic activity. Its mechanism of action is similar to that of imidacloprid. It mainly acts on the post-junction membrane of insect nerves. By combining with nicotinic acid acetylcholine receptors, it interferes with the normal conduction of the insect nervous system, causing blockage of nerve channels, resulting in a lar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N25/26A01N25/10A01N47/40A01P7/04
CPCA01N25/10A01N25/26A01N47/40
Inventor 温俊宝季英超刘彦雪雷建都李晓敏郑丹田雅洁
Owner BEIJING FORESTRY UNIVERSITY
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