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Diluent used for inactive porcine parvovirus vaccine

A parvovirus and live vaccine technology, applied in the directions of viruses, virus antigen components, viruses/phages, etc., can solve the problems of reducing the immune effect of live vaccines against porcine parvovirus disease, the uniformity of immune titers, and the large differences in vaccines. , to achieve the effect of improving the immune effect, improving the overall immune level, and enhancing the immune performance

Active Publication Date: 2017-05-31
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Porcine parvovirus disease live vaccine needs to be diluted with vaccine diluent when used. At present, water for injection or normal saline is usually used, but the immune titer of the vaccine diluted in this way is not high, and the difference between different batches of vaccines is large. , and there are stress reactions and allergic reactions after inoculation of diseased pigs, which greatly reduces the immune effect of porcine parvovirus disease live vaccine

Method used

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  • Diluent used for inactive porcine parvovirus vaccine
  • Diluent used for inactive porcine parvovirus vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. The preparation of Forsythia suspense extract includes the following steps:

[0024] Weigh honeysuckle and forsythia by weight 1:1, mix, add 10 times of 60% ethanol, ultrasonically extract for 15 minutes, collect the extract, repeat the extraction 3 times, mix the obtained extract, evaporate and concentrate, freeze-dry, that is Forsythia suspense extract is available.

[0025] 2. Preparation of Lycium barbarum polysaccharides includes the following steps:

[0026] (1) Dry the wolfberry in a 60°C thermostat to a constant weight, and crush it for later use;

[0027] (2) Pretreatment: Weigh the wolfberry crushed in step (1), reflux with 90% ethanol for 1 hour, discard the solvent, collect the residue, and air-dry the residue for later use;

[0028] (3) Microwave extraction: Weigh the wolfberry residue prepared in step (2), add distilled water at a material-to-liquid ratio of 1:8, microwave extraction with 300W power for 10 minutes, centrifuge to remove the precipitate, and colle...

Embodiment 2

[0038] 1. The preparation of Forsythia suspense extract includes the following steps:

[0039] Weigh honeysuckle and forsythia by weight 1:1, mix, add 12 times 50% ethanol, ultrasonically extract for 10 minutes, collect the extract, repeat the extraction 3 times, mix the obtained extract, evaporate and concentrate, freeze-dry, that is Forsythia suspense extract is available.

[0040] 2. Preparation of Lycium barbarum polysaccharides includes the following steps:

[0041] (1) Dry the wolfberry in a 60°C thermostat to a constant weight, and crush it for later use;

[0042] (2) Pretreatment: Weigh the wolfberry crushed in step (1), reflux with 90% ethanol for 1 hour, discard the solvent, collect the residue, and air-dry the residue for later use;

[0043] (3) Microwave extraction: Weigh the wolfberry residue prepared in step (2), add distilled water at a material-to-liquid ratio of 1:15, microwave extraction with 350W power for 5 minutes, centrifuge to remove the precipitate, and collect ...

Embodiment 3

[0053] 1. The preparation of Forsythia suspense extract includes the following steps:

[0054] Weigh honeysuckle and forsythia by weight 1:1, mix, add 11 times the amount of 55% ethanol, ultrasonically extract for 13 minutes, collect the extract, repeat the extraction 3 times, mix the obtained extract, evaporate and concentrate, freeze-dry, that is Forsythia suspense extract is available.

[0055] 2. Preparation of Lycium barbarum polysaccharides includes the following steps:

[0056] (1) Dry the wolfberry in a 60°C thermostat to a constant weight, and crush it for later use;

[0057] (2) Pretreatment: Weigh the wolfberry crushed in step (1), reflux with 90% ethanol for 1 hour, discard the solvent, collect the residue, and air-dry the residue for later use;

[0058] (3) Microwave extraction: Weigh the wolfberry residue prepared in step (2), add distilled water at a material-to-liquid ratio of 1:10, microwave extraction with 330W power for 8 minutes, centrifuge to remove the precipitate...

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Abstract

The invention belongs to the technical field of veterinary biological products, and concretely relates to a diluent used for an inactive porcine parvovirus vaccine. Every 1000 ml of a phosphate buffer solution with the pH value of 7 includes 1-5 g of honeysuckle flower and Weeping Forsythia extract, 5-10 g of wolfberry fruit polysaccharides, 20-80 mg of astragaloside II and 1-5 g of polyinosinic-polycytidylic acid. The diluent used for the inactive porcine parvovirus vaccine has the advantages of convenience in production, low cost, convenience and safety in use, reduction of the difference among different batches of vaccines, and improvement of the integral immunizing effect of the vaccine.

Description

Technical field [0001] The invention belongs to the technical field of veterinary biological products, and specifically relates to a diluent for porcine parvovirus disease live vaccines. Background technique [0002] Porcine parvovirus (Porcine parvovirus, PPV) belongs to the Parvovirus family (Paruovirdae) and is one of the main pathogens causing reproductive disorders in pigs, especially the reproductive disorders of primiparous sows, which are manifested in sows. The conception rate decreases, the number of live births decreases and / or premature births, miscarriages, deformities, stillbirths, mummified fetuses, weak babies, etc. occur. The virus can survive in the environment for several months, and has strong resistance to most disinfectants. At present, the virus has spread all over the world, causing huge economic losses to the pig industry and severely hindering the pig industry. healthy growth. PPV has a single serotype, and immunization is the main measure to prevent t...

Claims

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Application Information

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IPC IPC(8): A61K36/634A61K47/02A61P37/04A61K31/715A61K31/7048A61K31/713A61K39/23
CPCA61K31/7048A61K31/713A61K31/715A61K36/355A61K36/634A61K39/12A61K47/02A61K2236/333A61K2236/39C12N2750/14334A61K2300/00
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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