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A kind of method utilizing Bacillus subtilis fermentation to prepare tailings eluent

A technology of Bacillus subtilis and eluent, which is applied in the field of mine tailings repair, can solve the problems of easily damaged soil structure, long repair period, high energy consumption, etc., and achieve improved leaching efficiency, easy availability of raw materials, and high leaching rate Effect

Inactive Publication Date: 2020-07-31
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical remediation is suitable for remediation of tailings with high pollution concentration and small area, but it is easy to damage the soil structure, with large engineering volume, high energy consumption and high cost, and has great limitations; biological remediation can reduce the migration of heavy metals in the environment, However, the repair period is long, and it is easily limited by climate, geological conditions, and tailings types; chemically reducing the bioavailability of heavy metals, but it is easy to damage the soil structure, and the amount of chemicals used is large, and the cost is high. It is only suitable for heavily polluted areas. Minor Tailings Remediation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A kind of preparation method of heavy metal lead polluted soil eluent proposed by the present invention comprises the following steps:

[0017] 1. Crush beans to a particle size of 2 mm, add water and mix them, then sterilize them at high temperature, add Bacillus subtilis powder after cooling to room temperature, and place them on a constant temperature shaker for 48 hours. 120rpm, to obtain the fermentation mixture;

[0018] 2. After the fermentation mixture is left to settle completely, the upper layer liquid is taken as the tailings eluent.

[0019] Get 5.000g of lead tailings powder, the lead concentration in this sample is 79000mg / kg, add 50ml of the tailings eluent prepared by the present invention, place on a shaker and vibrate at a speed of 150rpm for 4h, after standing for complete precipitation, take the The clear solution was used to detect the lead content in the solution. The results showed that the lead content in the solution reached 34180 mg / kg, that ...

Embodiment 2

[0021] A kind of preparation method of heavy metal lead polluted soil eluent proposed by the present invention comprises the following steps:

[0022] 1. Crush the soybean meal to a particle size of 3 mm, add water and mix it, then sterilize it at high temperature, add Bacillus subtilis powder after cooling to room temperature, and place it on a constant temperature shaker for 72 hours. The cultivation temperature of the constant temperature shaker is 30°C, and the speed of the constant temperature shaker is 150rpm, to obtain the fermentation mixture;

[0023] 2. After the fermentation mixture is left to settle completely, the upper layer liquid is taken as the tailings eluent.

[0024] Get 5.000g of lead tailings powder, the lead concentration in this sample is 79000mg / kg, add 50ml of the tailings eluent prepared by the present invention, place on a shaker and vibrate at a speed of 150rpm for 4h, after standing for complete precipitation, take the The clear solution was used...

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PUM

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Abstract

The invention discloses a method for preparing tailing leacheate through fermentation of bacillus subtilis. The method comprises the following steps that beans or bean pulp is smashed till the grain size is 2-3 mm, water is added and mixed with the smashed beans or bean pulp, and then high-temperature sterilization is carried out; bacillus subtilis powder is added after the mixture is cooled to the room temperature, the new mixture is put on a constant temperature shaking table to be cultivated for 48-72 hours, and mixed fermentation liquor is obtained; and after the mixed fermentation liquor settles completely through standing, supernatant is fetched, and thus the tailing leacheate is obtained. According to the method, raw materials are easily available, the preparation process is simple, the preparation cost is greatly reduced, and no secondary pollution is caused to tailing treatment.

Description

technical field [0001] The invention relates to the technical field of tailings restoration, in particular to a method for preparing tailings eluate by fermentation of Bacillus subtilis. Background technique [0002] my country is a big mining country. The mining and utilization of minerals has greatly promoted economic development, but at the same time it has brought about great environmental pollution problems. These problems are mainly caused by the large amount of heavy metal pollutants contained in the tailings slag. Tailings are one of the wastes of mining and smelting. Its stacking not only occupies royal land resources, but also causes great pollution and damage to the surrounding environment, resulting in a decline in the quality of the mining area and surrounding soil, destroying the normal function of the ecosystem, and endangering human health. The accumulation of tailings has seriously damaged the natural ecological environment of land resources and damaged the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/02
CPCB09C1/02
Inventor 熊华斌赵霞高云涛李晓芬
Owner YUNNAN MINZU UNIV
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