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Embedded nano-iron/composite microbial agent and preparation method thereof

A technology of composite microbial bacterial agent and nano-iron, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor stability of nano-zero-valent iron, achieve low microbial toxicity, enhance degradation efficiency, and good The effect of biocompatibility

Active Publication Date: 2020-07-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although nano-zero valent iron has many advantages, it still encounters some problems in the process of its application, such as the poor stability of nano-zero valent iron.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Preparation of triclosan-degrading bacterial liquid

[0038]Agrobacterium sp., Enterobactercloacae., Bacillus sp., Gordonia sp., Pseudomonasputida. Pseudomonas (Pseudomonas stutzeri.) 2 rings were transferred to 30mL nutrient solution, the bacteria were cultured at 35°C for 2 days, and inoculated into the proliferation medium container at a volume ratio of 10%, at 35 Cultivate for 2 days under the condition of ℃, centrifuge at a speed of 5000rpm for 15min, and obtain the logarithmic growth phase cells of the above bacteria respectively. The logarithmic growth phase cells of the above-mentioned thalli are taken out, and with phosphate buffer (its main component sodium chloride 9.0g / L, potassium chloride 0.3g / L, dipotassium hydrogen phosphate 1.2g / L and potassium dihydrogen phosphate 0.3g / L, the rest is water) and washed twice. By volume percentage, get 5% Agrobacterium, 5% Bacillus, 10% Enterobacter cloacae, 20% Gordonella, 20% Pseudomonas putida and 40% Pseudomona...

Embodiment 2

[0052] (1) Preparation of triclosan-degrading bacterial liquid

[0053] Agrobacterium sp., Enterobactercloacae., Bacillus sp., Gordonia sp., Pseudomonasputida. Pseudomonas (Pseudomonas stutzeri.) 2 rings were transferred to 30mL nutrient solution, the bacteria were cultured at 35°C for 2 days, and inoculated into the proliferation medium container at a volume ratio of 10%, at 35 Cultivate for 2 days under the condition of ℃, centrifuge at a speed of 5000rpm for 15min, and obtain the logarithmic growth phase cells of the above bacteria respectively.

[0054] The logarithmic growth phase cells of the above-mentioned thalli are taken out, and with phosphate buffer (its main component sodium chloride 9.0g / L, potassium chloride 0.3g / L, dipotassium hydrogen phosphate 1.2g / L and potassium dihydrogen phosphate 0.3g / L, the rest is water) and washed twice. By volume percentage, get 5% Agrobacterium, 5% Bacillus, 10% Enterobacter cloacae, 20% Gordonella, 20% Pseudomonas putida and 40% ...

Embodiment 3

[0068] (1) Preparation of triclosan-degrading bacterial liquid

[0069] Agrobacterium sp., Enterobactercloacae., Bacillus sp., Gordonia sp., Pseudomonasputida. Pseudomonas (Pseudomonas stutzeri.) 2 rings were transferred to 30mL nutrient solution, the bacteria were cultured at 35°C for 2 days, and inoculated into the proliferation medium container at a volume ratio of 10%, at 35 Cultivate for 2 days under the condition of ℃, centrifuge at a speed of 5000rpm for 15min, and obtain the logarithmic growth phase cells of the above bacteria respectively.

[0070]The logarithmic growth phase cells of the above-mentioned thalli are taken out, and with phosphate buffer (its main component sodium chloride 9.0g / L, potassium chloride 0.3g / L, dipotassium hydrogen phosphate 1.2g / L and potassium dihydrogen phosphate 0.3g / L, the rest is water) and washed twice. By volume percentage, get 5% Agrobacterium, 5% Bacillus, 10% Enterobacter cloacae, 20% Gordonella, 20% Pseudomonas putida and 40% P...

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Abstract

The invention relates to an embedded nano iron / composite microbial agent and a preparation method thereof. The preparation method comprises the steps of firstly using agrobacterium tumefaeiens, enterobacter cloacae, bacillus, gordonia, pseudomonas putida and pseudomonas stutzeri to prepare a nano iron solution B, preparing embedding medium agar, PVA (Polyvinyl Alcohol) and a SiO2 solution C, and preparing a cross-linking agent aluminum sulfate saturated boric acid solution D; according to volume ratio, respectively taking and adding 15-18% of a solution B and 6-15% of thallus A into 57-66% of the solution C, stirring and mixing evenly, dropwise adding the mixture into 1-22% of a solution D at room temperature in a nitrogen protected environment, carrying out crosslinking treatment, rinsing and preserving to obtain the nano iron / composite microbial agent. The embedded nano iron / composite microbial agent takes advantage of a synergistic effect between the nano iron and microorganisms to improve the degradation efficiency of triclosan; and the microbial agent prepared is high in strength, small in microbial toxicity and low in raw material source cost, and can be used for treatment of a water body polluted by triclosan.

Description

technical field [0001] The invention relates to the field of triclosan wastewater treatment, in particular to an embedded nano-iron / composite microbial agent for degrading triclosan and a preparation method thereof. Background technique [0002] Triclosan has a good sterilization and disinfection effect, and has good safety, and even has the effect of promoting human skin metabolism, brightening and moisturizing. Since it was used in soap production in the 1970s, the application scope of triclosan has gradually expanded, and it has been widely used in the production process of personal care products and pharmaceuticals, such as detergents, deodorants, cosmetics, disinfection equipment, and Disinfection and sterilization of textiles before leaving the factory, etc. Triclosan is a polar hydrophobic organic matter, which is easy to deposit in solid phase substances such as soil and bottom mud. The lipophilicity of hydrophobic substances makes it easy to accumulate in organism...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/10C12N11/084C12N11/14C12N1/20C02F3/34C12R1/01C12R1/07C12R1/38C12R1/40C02F101/36C02F101/34
CPCC02F1/705C02F3/34C02F2101/345C02F2101/36C12N1/20C12N11/08C12N11/10C12N11/14
Inventor 陈元彩张振
Owner SOUTH CHINA UNIV OF TECH
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