Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Embedded nano-iron/single microorganism compound bacterial agent and preparation method thereof

A composite bacterial agent and nano-iron technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor stability of nano-zero-valent iron, achieve low microbial toxicity, realize in-situ repair, use Simple and convenient effects

Active Publication Date: 2020-02-18
SOUTH CHINA UNIV OF TECH
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although nano-zero valent iron has many advantages, it still encounters some problems in the process of its application, such as the poor stability of nano-zero valent iron.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Preparation of triclosan-degrading bacterial liquid

[0035] Pick 2 rings of Pseudomonas stutzeri., transfer them to 30mL nutrient solution, culture the bacteria at 35°C for 2 days, and inoculate Pseudomonas stutzeri at a volume ratio of 10% Bacteria proliferation medium container, then cultivated at 35°C for 2 days, centrifuged at 5000rpm for 15min, and obtained the logarithmic growth phase cells of the above bacteria;

[0036] The logarithmic growth phase cells of the above-mentioned thalli are taken out, and with phosphate buffer (its main component sodium chloride 9.0g / L, potassium chloride 0.3g / L, dipotassium hydrogen phosphate 1.2g / L and potassium dihydrogen phosphate 0.3g / L, the rest is water) after washing twice, press and suspend in normal saline, refrigerate at 4°C for later use, and record it as cell A;

[0037] The nutrient solution for Pseudomonas stutzeri was beef extract 6.0g / L, NaCl 5.0g / L, peptone 10.0g / L, soybean powder 2.0g / L, pH 6.5, and the res...

Embodiment 2

[0054] (1) Preparation of triclosan-degrading bacterial liquid

[0055] Pick 2 rings of Pseudomonas stutzeri (Pseudomonas stutzeri.) (source is the same as in Example 1), which are transferred to 30mL nutrient solution respectively, and the bacteria are cultivated at 35°C for 2 days, with a volume ratio of 10%. Inoculate into the container of the proliferation medium of Pseudomonas stutzeri, then culture at 35°C for 2 days, centrifuge at a speed of 5000rpm for 15min, and obtain the logarithmic growth phase cells of the above bacteria;

[0056] The logarithmic growth phase cells of the above-mentioned thalli are taken out, and with phosphate buffer (its main component sodium chloride 9.0g / L, potassium chloride 0.3g / L, dipotassium hydrogen phosphate 1.2g / L and potassium dihydrogen phosphate 0.3g / L, the rest is water) after washing twice, press and suspend in normal saline, refrigerate at 4°C for later use, and record it as cell A;

[0057] The nutrient solution for Pseudomonas ...

Embodiment 3

[0073] (1) Preparation of triclosan-degrading bacterial liquid

[0074] Pick 2 rings of Pseudomonas stutzeri (Pseudomonas stutzeri.) (source is the same as in Example 1), which are transferred to 30mL nutrient solution respectively, and the bacteria are cultivated at 35°C for 2 days, with a volume ratio of 10%. Inoculate into a container of proliferation medium, then culture at 35°C for 2 days, centrifuge at a speed of 5000rpm for 15min, and obtain the logarithmic growth phase cells of the above bacteria;

[0075] The logarithmic growth phase cells of the above-mentioned thalli are taken out, and with phosphate buffer (its main component sodium chloride 9.0g / L, potassium chloride 0.3g / L, dipotassium hydrogen phosphate 1.2g / L and potassium dihydrogen phosphate 0.3g / L, the rest is water), washed twice, pressed and suspended in normal saline, and refrigerated at 4°C for later use. Denoted as cell A;

[0076] The nutrient solution for Pseudomonas stutzeri was beef extract 6.0g / L...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an embedded nano-iron / single-microorganism composite microbial agent and a preparation method thereof. The preparation method comprises the following steps: preparing pseudomonas stutzeri thallus to obtain thallus A, preparing a nano-iron solution B, preparing an embedding medium agar, PVA (poly vinyl alcohol) and SiO2 solution C, preparing a cross-linking agent aluminum sulfate saturated boric acid solution D, adding 15 to 18 percent of the solution B and 6 to 15 percent of the thallus A into 57 to 66 percent of the solution C according to a volumetric ratio under the condition of a 50 to 70 DEG C constant temperature water bath, performing uniform stirring and mixing, adding dropwise the mixed solution into 1 to 22 percent of the solution D at room temperature in a nitrogen protection environment, and performing cross-linking treatment, washing and storage to obtain the nano-iron / single-microorganism composite microbial agent. According to the embedded nano-iron / single-microorganism composite microbial agent and the preparation method thereof, the degradation efficiency of triclosan is improved by virtue of the synergistic effects of nano-iron and a microorganism; the prepared microbial agent is high in intensity, low in microorganism toxicity, extensive in raw material source and low in cost, and can be widely applied to treatment of a triclosan-contaminated water body.

Description

technical field [0001] The invention relates to the field of triclosan wastewater treatment, in particular to an embedded nano-iron / single microorganism composite bacterial agent for degrading triclosan and a preparation method thereof. Background technique [0002] Triclosan has a good sterilization and disinfection effect, and has good safety, and even has the effect of promoting human skin metabolism, brightening and moisturizing. Since it was used in soap production in the 1970s, the application scope of triclosan has gradually expanded, and it has been widely used in the production process of personal care products and pharmaceuticals, such as detergents, deodorants, cosmetics, disinfection equipment, and Disinfection and sterilization of textiles before leaving the factory, etc. Triclosan is a polar hydrophobic organic matter, which is easy to deposit in solid phase substances such as soil and bottom mud. The lipophilicity of hydrophobic substances makes it easy to a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/084C12N11/04C12N1/20C12R1/38
CPCC12N11/04C12N11/08C12N11/14
Inventor 陈元彩黎良浩
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com