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Transcription inhibitor family gene and application thereof

A gene and plant technology, applied in the application field of improving plant stress resistance, can solve problems such as restricting the commercial value of transcription factors

Pending Publication Date: 2017-05-31
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, gene overexpression involves regulatory issues in transgenic plants, which severely restricts the commercial availability of transcription factors known to be involved in ABA signaling

Method used

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  • Transcription inhibitor family gene and application thereof
  • Transcription inhibitor family gene and application thereof
  • Transcription inhibitor family gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Acquisition of Arabidopsis AITRs

[0020] According to the transcriptome data measured by the inventors, it was found that the expressions of six related genes with unknown functions, At3g27250, At3g48510, At5g40790, At5g40800, At5g50360 and At5g63350, were all induced by ABA. The qRT-PCR results also proved that exogenous ABA treatment can induce 6 genes. And the expression of these genes in the ABA synthesis mutant aba1-5 was significantly reduced (attached figure 1 ). Through amino acid sequence analysis, it is found that the amino acid sequence of At3g27250 has the transcription repression domain LxLxL. The protoplast transfection test of Arabidopsis found that the proteins encoded by all six genes are located in the nucleus and can also inhibit the expression of reporter genes. In order to reflect its characteristics, the inventor named them ABA-induced transcription repressor1 (AITR1), AITR2, AITR3, AITR4, AITR5 and AITR6 (attached figure 2 ). The results indicate ...

Embodiment 2

[0022] Acquisition of AITRs in different plants

[0023] Using the complete amino acid sequence of AITR1 to perform BLAST detection on the NCBI website, it was found that only plants have its homologous protein. Using the complete amino acid sequence of AITR1 to perform BLAST detection on each plant on the phytozome website, it is found that only angiosperms have their homologous protein coding genes (attached image 3 ), and there are different numbers of homologous protein-coding genes in all angiosperms. A total of 162 homologous protein-coding genes were found in 42 plants except Arabidopsis. See Table 1 for relevant information about AITRs coding genes of 43 different plants, including Arabidopsis. The results indicate that AITRs are in a family of proteins that are conserved in angiosperms.

[0024] Table 1. Distribution of AITRs in angiosperms

[0025] Table 1.AITRs in angiosperms

[0026]

[0027]

Embodiment 3

[0029] Detection of transcriptional activity of soybean and tomato AITRs

[0030] In order to test whether AITRs genes in other plants are also induced by ABA, the inventors took tomato as an example to test and found that the expression of AITRs genes in 3 tomatoes was induced by ABA. The inventors further used protoplast transfection for detection and found that the AITRs of the three tomatoes were also located in the nucleus and could inhibit the expression of reporter genes. Similarly, the AITRs of 6 soybeans are also transcription repressors (attached Figure 4 ). The results indicate that AITRs are a new family of transcription inhibitors conserved in angiosperms.

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Abstract

The invention relates to discovery of a transcription factor family gene specific to angiosperms, detection on transcription inhibition activity of an encoding protein, and application of the transcription factor family gene in improving plant stress resistance. An experiment on AITRs (ABA-induced transcription repressors) gene knocked-out arabidopsis mutant is utilized to verify enhanced resistance of the mutant to ABA and an abiotic stress. AITRs are further discovered to be widely present in the angiosperms. Based on experiments taking tomatoes and soybeans as examples, expression of AITRs gene of the tomatoes is induced by the ABA, and a protoplast transfection experiment proves that the AITRs of both the tomatoes and the soybeans are transcription inhibitors, which indicates that the AITRs are a novel transcription factor family conserved in the angiosperms, and knocking out corresponding AITRs gene of a plant can enhance resistance of the plant to the abiotic stress. Therefore, the invention provides a transcription factor gene family which can be applied to genetic modification to enhance resistance of the plant to adverse environment.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and relates to the discovery of a type of angiosperm-specific transcription factor family genes, the detection of the transcription inhibitory activity of its encoded proteins, and its application in improving plant stress resistance. First, it was confirmed in Arabidopsis that the gene encodes a new family of transcription inhibitors. It was found that it is widely present in angiosperms by homologous comparison of amino acid sequences. The gene knockout can improve the stress resistance of plants, which can Applied to plant improvement. Background technique [0002] Adversity stresses, including abiotic stresses such as salt-alkali, drought, and cold damage, and biological stresses such as pests and diseases, affect the growth and development of plants, and are one of the biggest constraints on agricultural and forestry production. Therefore, improving plant resistance to adversity through gene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00
CPCC12N15/8271C07K14/415C12N9/22C12N15/8213C12N15/8273C12N2310/20C12N15/11C12N2800/80
Inventor 王树才田海南陈思羽王天雅郑凯杰柳善达李冬秋
Owner NORTHEAST NORMAL UNIVERSITY
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