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Preparation method of paraffin section of cannabis sativa callus

A technology of callus and paraffin sectioning, which is applied in the preparation of test samples, etc., can solve the problems of low efficiency, time-consuming and labor-consuming paraffin sectioning technology

Active Publication Date: 2017-05-31
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no technology specifically applied to the preparation of hemp tissue plant slices. Generally, 20-40 slices are made for each fixed tissue, and each slice needs to be stained one by one, which is time-consuming and labor-intensive, resulting in inefficient use of conventional paraffin section techniques. high

Method used

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  • Preparation method of paraffin section of cannabis sativa callus
  • Preparation method of paraffin section of cannabis sativa callus

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Experimental program
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Effect test

Embodiment 1

[0039] The preparation method of the paraffin section of the hemp callus of the present embodiment may further comprise the steps:

[0040] 1. Material collection and fixation:

[0041] (1) Select the marijuana callus material after culturing for 5 days, wash it with running water, and remove the medium and other sundries;

[0042] (2) absorb the moisture on the surface of the hemp callus with filter paper;

[0043] The cannabis callus material was immersed in the improved FAA fixative solution and fixed for 24 hours; in order to make the fixative solution penetrate into the cannabis callus tissue better, the fixed material was placed in an aspirating device and pumped for 1 hour;

[0044] Because of the tenderness of the hemp callus material, in order to prevent the material from shrinking, the FAA fixative has been improved. The improved FAA fixative of the present invention is: formaldehyde solution (formalin) with a mass concentration of 38%, glacial acetic acid and The ...

Embodiment 2

[0068] The preparation method of the paraffin section of the hemp callus of the present embodiment may further comprise the steps:

[0069] 1. Material collection and fixation:

[0070] (1) Select the marijuana callus material after culturing for 9 days, wash it with running water, and remove the medium and other sundries;

[0071] (2) absorb the moisture on the surface of the hemp callus with filter paper;

[0072] The cannabis callus material was immersed in the improved FAA fixative solution and fixed for 24 hours; in order to make the fixative solution penetrate into the cannabis callus tissue better, the fixed material was placed in an aspirating device and pumped for 1 hour;

[0073] Because the hemp callus material is young and tender, in order to prevent the material from shrinking, the FAA fixative has been improved. The improved FAA fixative of the present invention is: formaldehyde solution (formalin) with a mass concentration of 40%, glacial acetic acid and The e...

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Abstract

A preparation method of a paraffin section of cannabis sativa callus comprises the following steps: 1, preparing materials and fixing; 2, completely dyeing; 3, dehydrating; 4, transparentizing; 5, waxing and embedding; 6, sectioning, expanding the section and adhering the section; 7, dewaxing; and 8, sealing the section and observing. According to the preparation method of the paraffin section of the cannabis sativa callus, an existing plant sectioning technology is improved; the components of FAA stationary liquid are modified; the steps of pumping air after fixing and before dyeing are added; a complete dyeing method is carried out once after the fixing is finished; weak aqua ammonia is used for quickly recovering blue; the step of dyeing after sectioning is saved; the paraffin section with complete and clear structure of the cannabis sativa callus can be obtained through the method; compared with an existing plant sectioning technology, the method for preparing the paraffin section of the cannabis sativa callus has the advantages of being appropriate, and high in efficiency.

Description

technical field [0001] The invention relates to a method for making a paraffin section, in particular to a method for making a paraffin section of a hemp callus. Background technique [0002] Hemp (Cannabis Sativa L.) is a natural fiber plant with excellent environmental adaptability and strong ability to absorb heavy metals. It plays an important role in textiles, pharmaceuticals, building materials, energy and other fields and has broad development prospects (Xiong Heping, 2008). Hemp fiber has the advantages of high stretchability, good shape retention, good hygroscopicity, strong antibacterial and antistatic capabilities, etc. With the depletion of important natural resources such as global oil and coal, hemp, as an important natural fiber plant, will play an increasingly important role. The planting area and average output of hemp for fiber in my country rank among the top in the world. [0003] Cannabis is a frequently dioecious and often cross-pollinated crop with ...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 程超华粟建光赵立宁李育君
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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