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Method for detecting content of total saponins in balsam pear leaves

A detection method, the technology of total saponins, applied in the field of detection of medicinal material quality, can solve the problems of lack of quality evaluation methods and standards of bitter melon leaves, and achieve the effect of clinical curative effect guarantee and quality monitoring

Inactive Publication Date: 2017-05-31
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are many multi-angle studies on bitter melon fruit at home and abroad, but little research on its leaves, and there is still a lack of quality evaluation methods and standards for bitter melon leaves.

Method used

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  • Method for detecting content of total saponins in balsam pear leaves
  • Method for detecting content of total saponins in balsam pear leaves
  • Method for detecting content of total saponins in balsam pear leaves

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Experimental program
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Effect test

Embodiment 1

[0041] (1) Examination of the determination method of total saponin content

[0042] 1 reference substance solution

[0043] Reference substance solution: take ginsenoside Rg dried to constant weight 1 Appropriate amount of reference substance, accurately weighed, placed in a 10mL volumetric flask, added methanol, constant volume, made 0.5mg / mL ginsenoside Rg 1 solution.

[0044] 2 Measurement wavelength inspection

[0045] Accurately measure 0.125mL ginsenoside Rg 1 Put the reference substance solution in a 10mL stoppered graduated test tube, evaporate the solvent in a water bath, add 0.2mL of newly prepared 5% vanillin glacial acetic acid solution, 2.0mL of perchloric acid, shake well, and close the plug. The results show that at 558nm, ginseng Saponin Rg 1 The reference substance solution has the maximum absorption.

[0046] Therefore, the measurement wavelength was set to 558 nm.

[0047] 3 color method examination

[0048] 3.1 Examination of the dosage of vanillin...

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Abstract

The invention discloses a method for detecting the content of total saponins in balsam pear leaves. The method comprises the following steps: (1) establishment of a standard curve: (a) preparation of a reference solution; and (b) measurement of the reference solution; and (2) measurement of the content of total saponins in a to-be-detected sample: (c) preparation of a test solution; and (d) measurement of the test solution. In the method for detecting the content of total saponins in balsam pear leaves, disclosed by the invention, through screening of multiple technologies, the content of total saponins in balsam pear leaves can be detected accurately, reliably and simply, so that the quality of the medicinal material balsam pear leaves is effectively monitored, and the clinical effect of the balsam pear leaves is effectively guaranteed.

Description

technical field [0001] The invention relates to a method for detecting the quality of medicinal materials, in particular to a method for detecting the content of total saponins in bitter gourd leaves. Background technique [0002] Bitter melon leaves are the dried leaves of the annual vine herb Momordica charantia L. of the family Cucurbitaceae. Cool in nature, bitter in the mouth. It has the effects of clearing heat and detoxifying, sterilizing, stopping dysentery and relieving pain, and can treat stomach pain, dysentery, boils and swollen toxins. As a common folk medicine, bitter gourd leaves are widely used. They are often used to treat redness, swelling and heat pain of the skin after mosquito bites, prickly heat in children, heat ulcers, red bayberry sores, dog bites and goosefoot. [0003] Bitter gourd leaves are rich in resources, have definite curative effects, are widely used by folks, are highly recognized, are convenient and easy to obtain, and have good develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N1/28G01N1/34
CPCG01N21/33G01N1/28G01N1/34
Inventor 蒋桂华于宗强连艳刘晓芬王少男兰群高必兴
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE