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Method for measuring content of polysaccharides in glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation

A technique for righting and determination of Zhenqi, which is applied in the direction of color/spectral property measurement, etc., can solve the problems of affecting the determination result of polysaccharide content, high extraction cost, unguaranteed drug quality, etc.

Inactive Publication Date: 2014-02-05
GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing quality inspection method of Zhenqi Fuzheng preparation does not measure the content of polysaccharides, and the quality inspection standard is not perfect
Moreover, the extraction of polysaccharides mainly adopts the water extraction process. The disadvantage of this process is that the yield of crude polysaccharides is low and the extraction cost is high, which will inevitably affect the determination results of polysaccharides.
If there is no strict and accurate quality standard, the quality of the obtained drug cannot be guaranteed, which will definitely affect the clinical efficacy of the drug

Method used

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  • Method for measuring content of polysaccharides in glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation
  • Method for measuring content of polysaccharides in glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation
  • Method for measuring content of polysaccharides in glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Example 1 of the present invention: The method for determining the content of polysaccharides in Zhenqi Fuzheng preparation (phenol-sulfuric acid method) includes the following steps:

[0098] (1) Preparation of the reference substance solution: take an appropriate amount of anhydrous glucose reference substance, weigh it accurately, add water to make 1mL of a solution containing 0.1mg, and obtain it;

[0099] (2) Preparation of the test solution: Accurately weigh 2.0 g of Zhenqi Fuzheng preparation or its contents, accurately add 50 mL of water, weigh, ultrasonically, reflux for 30-60 min or microwave extraction for 5-15 min, make up the weight, filter Precisely measure 25mL of the filtrate, add 95% ethanol to the filtrate to make the alcohol content reach 80%, filter, wash the precipitate with 95% ethanol, evaporate the precipitate, add water to dissolve and transfer to a 25mL volumetric flask, ultrasonic 10 ~30min, dilute to the mark, shake well, and you get it;

[...

Embodiment 2

[0103] Example 2 of the present invention: The method for determining the content of polysaccharides in Zhenqi Fuzheng preparation (phenol-sulfuric acid method) includes the following steps:

[0104] (1) Preparation of the reference substance solution: take an appropriate amount of anhydrous glucose reference substance, weigh it accurately, add water to make 1mL of a solution containing 0.1mg, and obtain it;

[0105] (2) Preparation of the test solution: Accurately weigh 2.0 g of Zhenqi Fuzheng preparation or its contents, accurately add 50 mL of water, weigh it, and make up the weight by ultrasonication, reflux for 30-60 min or microwave extraction for 5-15 min; 3000 Centrifuge at ~5000rpm for 3min, take the supernatant, and get it;

[0106] (3) Preparation of 5% phenol reagent: Take an appropriate amount of phenol, add 0.1g of aluminum flakes and 0.05g of sodium bicarbonate, distill, collect the fraction at 180°C, weigh 7.5g, add 150mL of water to dissolve, put it in a brown...

Embodiment 3

[0109] Example 3 of the present invention: The method for determining the content of polysaccharides in Zhenqi Fuzheng preparation (phenol-sulfuric acid method) includes the following steps:

[0110] (1) Preparation of the reference substance solution: take an appropriate amount of anhydrous glucose reference substance, weigh it accurately, add water to make 1mL of a solution containing 0.1mg, and obtain it;

[0111] (2) Preparation of the test solution: Accurately weigh 6.0 g of Zhenqi Fuzheng preparation or its contents, put it in a 250mL flask, extract it with petroleum ether, diethyl ether and 90% ethanol in sequence at 60-90°C, and evaporate the residue to dryness , accurately add 150mL of water, weigh, ultrasonically, reflux for 30-60min or microwave extraction for 5-15min, make up the weight, and filter; accurately measure 100mL of the subsequent filtrate, add activated carbon, decolorize, filter, accurately measure 50mL of the subsequent filtrate, Add 95% ethanol to ma...

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Abstract

The invention discloses a method for measuring the content of polysaccharides in a glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation. The method takes anhydrous dextrose as a reference substance and can be used for measuring the content of the polysaccharides in the glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation at the wavelength being 480-490nm by using a phenol-sulfuric acid method or at the wavelength being 600-625nm by using an anthrone-sulfuric acid method. The method is simple and convenient to operate, good in quick reproducibility, high in recovery rate and sensitivity and accurate in measurement result, and can be used for effectively controlling the quality of the glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation so as to ensure a clinical treatment effect of the glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation.

Description

technical field [0001] The invention relates to a method for determining the content of polysaccharides in Zhenqi Fuzheng preparation, belonging to the technical field of traditional Chinese medicine quality detection. Background technique [0002] Zhenqi preparations have the effects of invigorating qi and nourishing yin, strengthening the body and strengthening the foundation. Modern pharmacological experiments have proved that it can improve the immune function of the human body, protect the function of bone marrow and adrenal cortex, and promote the production of interferon; cooperate with radiotherapy and chemotherapy of tumor patients, reduce the toxic and side effects of patients during radiotherapy and chemotherapy, and promote the recovery of normal functions. Its prescription consists of Astragalus and Ligustrum lucidum. At present, the dosage forms of Zhenqi preparation for clinical selection include tablets, capsules, granules and dropping pills. [0003] Astra...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/33
Inventor 张观福
Owner GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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