Method for quickly detecting microsporidia spores

A technology for sporozoite spores and spores, which is applied in the field of direct detection of pathogenic microsporidia spores based on single-cell Raman spectroscopy, can solve the problems of detection hysteresis, toxicity, and low detection sensitivity, and achieve a good signal-to-noise ratio and easy operation Effect

Inactive Publication Date: 2017-05-31
王桂文
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Problems solved by technology

Although optical microscopy is relatively reliable and simple, its detection sensitivity is low, and generally it can only be observed in the late stage of infection, and there is a huge lag in detection
Electron microscopic examination must be performed after ultra-thin sectioning, which has relatively high technical requirements for technical equipment and operators. Currently, it is mainly used for scientific research, and it cannot be used for practical diagnosis and identification.
Although the detection method of microsporidia in molecular biology has great advantages over the traditional microscope detection method in terms of sensitivity and specificity, this method requires high experimental skills for operators, and the method used for staining the observation results The dye solution is also highly toxic to the human body

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  • Method for quickly detecting microsporidia spores
  • Method for quickly detecting microsporidia spores
  • Method for quickly detecting microsporidia spores

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Embodiment Construction

[0030] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. This embodiment is only to illustrate the present invention more clearly, rather than limit the present invention.

[0031] 1. Raman spectrum analysis of different silkworm microsporidia spores

[0032] 1. Experimental materials

[0033] Strains: isolated from silkworms infected with Nosema bombycis (N.b for short).

[0034] 2. Experimental method

[0035] 2.2 N.b infection process / method

[0036] Dilute the microsporidium spore liquid for testing to a concentration of 1×10 7 mL-1 Take the suspension of microspores and smear it on the surface of mulberry leaves, and feed the silkworms at the 3rd age. After the silkworms eat up the poisonous mulberry leaves, they will change to normal non-toxic mulberry leaves.

[0037] 2.3 N.b spore isolation

[0038] The dead silkworm body was taken, and the dissected silkworm midgut was ground with a mor...

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Abstract

The invention discloses a method for quickly detecting microsporidia spores based on single cell Raman tweezers, belongs to the technical field of pathogenic bacterial detection. The method mainly adopts a pair of laser tweezers and a single cell Raman spectrum technology to judge spores of pathogenic bacteria microsporidia in a host body or the natural environment. The method is easy and convenient to operate, no staining is needed; detection is directly carried out in germfree water; furthermore, whether a sample contains the microsporidia spores or not can be quickly and intuitively judged; therefore, the method is worthy of being popularized and is simple, convenient, efficient and reliable for detection of the microsporidia spores.

Description

technical field [0001] The invention belongs to the technical field of pathogenic bacteria detection, and in particular relates to a method for directly detecting spores of pathogenic microsporidia based on single-cell Raman spectrum. Background technique [0002] Microsporidia are a group of intracellular obligate parasitic unicellular eukaryotes currently classified as fungi. The life cycle of microsporidia can be divided into three stages: infection period (spore germination), reproduction period (schizogenesis) and sporulation period. Spores are the mature cells of Microsporidia, which spread with the excretion of host feces to infect new hosts, and the germination of spores is the key initiation of Microsporidia infecting hosts. [0003] Microsporidia have a wide range of hosts and can infect invertebrates and vertebrates including humans, causing serious diseases of many insects, aquatic animals and even primates. More than 150 genera and more than 1,400 species have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 王桂文黄旭华戴圣生黄深惠王晓春
Owner 王桂文
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