Method and test strip for rapid detection of avian influenza and human influenza susceptible population

Abstract

The invention discloses a method for rapidly detecting susceptible populations of bird flu and human flu and a test strip, including a sample pad, a gold standard pad, a detection and control area arranged in sequence along the chromatographic direction, and a pad for absorbing excess liquid in the detection sample. Water-absorbing pad; The detection and control area includes a detection zone and a quality control line arranged in sequence; The gold standard pad includes colloidal gold-labeled lectin MAL-II or SNA; A stable internal control lectin or a stable internal control lectin that does not bind to the lectin SNA. Insert one end of the sample pad of the test strip into the saliva sample or contain it in the oral cavity. After 10 to 15 minutes, take out the test strip, observe the color development state of the test strip and the quality control line, and obtain the test result. The invention truly realizes non-invasive , Safe and simple detection of avian influenza and human influenza virus susceptible populations.

Description

technical field [0001] The invention relates to rapid detection of avian influenza and human influenza virus-susceptible populations based on test strip technology, and can also be developed into a rapid detection of avian influenza and human influenza virus-susceptible populations by a double lectin sandwich method based on enzyme plate detection. Background technique [0002] Lectins are non-immune-derived, non-enzymatically active sugar-binding proteins that can specifically recognize and bind to a specific sequence of sugar chains in monosaccharides or polysaccharides with a special structure. Up to now, more than 100 plant-derived lectins have been found in nature. Lectins have various applications: such as the diagnosis of malignant tumors and other diseases, the design of antibacterial and antiviral drugs, the design of "biological missiles", and the identification and analysis of sugar chain structures, etc. With the introduction of microarray technology into the fi...

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Problems solved by technology

[0011] Chinese patent "A detection tool for screening avian influenza and human influenza virus susceptible population" (publication number: CN103884834A, publication date: 2014.06.25) discloses the relationship between lectin MAL-II and avian influenza susceptibility and lectin The relationship between SNA and human influenza virus susceptibility, and pointed out that the expression level of α2-3 or α2-6 linked terminal sialic acid sugar chain structure binding to the corresponding lectin MAL-II or SNA in the saliva of susceptible people was significantly down-regulated, However, this patent is only applied to chip kits, and the simplicity, speed and cost of detection need to be improved

[0012] The application of test strip detection technology in disease diagnosis and detection is gradually increasing, and it has the characteristics of rapidity and low price. However, there are no reports on test strips and detection methods for detecting avian influenza and human influenza susceptible populations.

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