30 results about "Bird flu (avian influenza)" patented technology

The invention discloses a method for rapidly detecting susceptible populations of bird flu and human flu and a test strip, including a sample pad, a gold standard pad, a detection and control area arranged in sequence along the chromatographic direction, and a pad for absorbing excess liquid in the detection sample. Water-absorbing pad; The detection and control area includes a detection zone and a quality control line arranged in sequence; The gold standard pad includes colloidal gold-labeled lectin MAL-II or SNA; A stable internal control lectin or a stable internal control lectin that does not bind to the lectin SNA. Insert one end of the sample pad of the test strip into the saliva sample or contain it in the oral cavity. After 10 to 15 minutes, take out the test strip, observe the color development state of the test strip and the quality control line, and obtain the test result. The invention truly realizes non-invasive , Safe and simple detection of avian influenza and human influenza virus susceptible populations.

The invention provides a rapid detection method for distinguishing the strong and weak viruses of H7N9 subtype avian influenza virus. By comparing the nucleotide sequences of the HA and NA genes of the H7N9 subtype avian influenza virus isolated in recent years, they are respectively designed in their conserved regions. Primers and probes were identified, and a real-time fluorescence quantitative RT-PCR nucleic acid rapid detection method that can distinguish between strong and weak H7N9 subtype avian influenza virus was established. The real-time fluorescent RT-PCR method for distinguishing the strong and weak viruses of the H7N9 subtype avian influenza virus provided by the present invention can detect and distinguish the strong and weak viruses of the H7 subtype avian influenza virus in the same reaction tube, and can also detect the N9 subtype bird flu virus. It has high sensitivity, short detection time, and does not require open links such as electrophoresis. It can be completed in a closed reaction tube with only one fluorescent PCR instrument, and the reaction curve can be viewed in real time during the detection process to make a quick judgment on the result.

The invention relates to a traditional Chinese veterinary medicine for resisting chicken Newcastle disease virus disease and avian influenza, which is prepared from the following components in parts by weight: 10-50 parts of honeysuckle, 10-50 parts of Houttuynia cordata, 6-20 parts of Daqingye, and 8 parts of forsythia ~40, Scutellaria baicalensis 10~40. The invention also discloses a preparation method of the traditional Chinese veterinary medicine against chicken Newcastle disease virus disease and bird flu. The composition of the invention has reasonable formula and synergistic effect, and has obvious control effect on chicken Newcastle disease virus disease and prevention and treatment of avian influenza; it is green, safe, stable, easy to transport and store; it is used for drinking water administration, convenient to use, and has good efficacy. The application and popularization prospect; the preparation method is simple; the resource-rich medicinal materials are used in the compound preparation, which reduces the cost; at the same time, no drug residues will be generated in chicken products, which is beneficial to ensuring food safety.

The invention provides a polypeptide antigen for preparing a bird flu synthetic peptide vaccine, a vaccine containing the polypeptide antigen and a preparation method thereof. Polypeptide antigens include the polypeptides shown in Sequence 1. The avian influenza synthetic peptide vaccine provided by the invention has good immune efficacy, and the antigen of the vaccine is a chemically synthesized polypeptide, does not contain avian influenza virus nucleic acid particles, and has high safety. Therefore, the vaccine of the invention can effectively deal with the antigenic variation of the avian influenza virus, has no biological safety, is easy to synthesize on a large scale, and has good application prospects.

The invention relates to the technical field of breeding fodder production, and discloses a fodder feeder capable of preventing avian influenza. The fodder feeder comprises a shell, multiple flow guiding holes are formed in the shell, clamping grooves are formed in the portion, outside the flow guiding holes, of the shell, the flow guiding holes are located in the bottom of the clamping grooves, the edges of the clamping grooves are hinged with clamping plates, a fodder ball against each clamping groove is arranged on the exterior of the shell, a pushing plate matched with each clamping groove is arranged between each fodder ball and the corresponding clamping groove, a medicine liquid ball is arranged in the shell, multiple through holes are formed in the medicine liquid ball, all the through holes are sealed by films, steel wires are arranged in the shell and wrapped with cotton threads, one end of each steel wire is fixed to each film, the other end of each steel wire penetrates the corresponding flow guiding hole and pushing plate and is fixed to the pushing plate, the end, penetrating the corresponding pushing plate, of each steel wire penetrates into the corresponding fodder ball , and the cotton threads are connected between the adjacent steel wires. By means of the fodder feeder, the defect can be overcome that existing fodder needs to be mixed with medicine manually, and medicine efficacy failure is prevented.

The present invention discloses a Chinese medicine feed additive for avian influenza prevention, which is characterized in that raw materials of the Chinese medicine feed additive comprise, by weight: 10 to 15 parts of hypericum perforatum, 10 to 15 parts of heartleaf houttuynia herb, 5 to 10 parts of folium perillae and 15 to 20 parts of rosa laevigata. According to the present invention, the Chinese medicine feed additive has effects of clearing heat, removing toxicity, fighting bacteria, eliminating inflammation, treating common cold, wind cold, aversion and fever of cold, and enhancing immunity. Adding the Chinese medicine feed additive to poultry feed can effectively prevent poultry from being infected with bird flu virus, and has no potential side effects on people who eat the poultry.

The invention relates to isolation, identification and application of a Fabricius bursa active hexapeptide for promoting an immunoreaction of avian influenza and / or a Newcastle disease bivalent vaccine. The Fabricius bursa active hexapeptide has an amino acid composition of Lys Gly Asn Arg Val Tyr, has a simple structure and is extremely weak in immunogenicity. The active peptide has the functionof promoting the immune response of the vaccine and can promote the antibody reaction against the AIV antigen and the antibody reaction against the NDV antigen in the mouse in the mouse immunization experiment. The active peptide can promote the cellular immune response, improve lymphocyte viability and an antigen presentation reaction and improve the vaccine immunopotency, can be used as a vaccine adjuvant or immunopotentiator in animal vaccine application research, can improve the immune response of animal organisms to specific antigens, can improve the immune efficacy of vaccines and can improve the ability of animal organisms resisting epidemic disease infection.

The invention relates to a veterinary pharmaceutical composition and a preparation method thereof. The veterinary pharmaceutical composition of the invention is a vaccine oral solution for the prevention and the treatment of bird flu of domestic animal and fowl, and is prepared by the raw materials according to the following parts by weight: 10 to 20 parts of forsythia suspensa, 10 to 25 parts ofasarum, 5 to 20 parts of ilex pubescens, 15 to 30 parts of rhizoma anemarrhenae, 8 to 20 parts of wild chrysanthemum, 10 to 20 parts of polygalae, 10 to 25 parts of lobelia chinensis, 2 to 5 parts ofsnake galls, 10 to 25 parts of ox to gall, 40 to 60 parts of aloe, 25 to 40 parts of rock sugar, 5 to 15 parts of rimantadine and 3 to 10 parts of azithromycin. The oral solution of the invention canbe directly used as daily feed for feeding domestic animals and fowl, which can effectively inhibit bird flu virus, reduce the infection rate of respiratory tract and lung and prevent domestic animaland fowl from being infected by bird flu.

The invention relates to a feed additive for preventing avian influenza; the feed additive is composed of the following raw materials in parts by weight: 30-60 parts of folium isatidis, 20-40 parts of common St.John's wort herb, 10-30 parts of radix astragali, and 10-25 parts of liquorice. The invention also discloses a preparation method of the feed additive. The feed additive not only can prevent and control avian influenza but also can improve the immunity of animals, and has the characteristics of small side effects, low cost, simple process and easy industrialization.

The invention discloses an avian influenza virus-like particle antigen, a vaccine and its preparation method and application. It is formed by self-assembly of a recombinant baculovirus transfected insect cells based on serial expression of H7N9 subtype avian influenza virus HA, NA, and M1 genes Bird flu virus-like particle antigen; the virus-like particle antigen prepared by the present invention is used to prepare vaccine. The vaccine prepared by the invention can produce higher titers of HI antibody, neutralizing antibody and IgY antibody after immunizing chickens, can provide 100% clinical protection against H7N9 subtype highly pathogenic avian influenza virus, and can effectively inhibit shedding . The invention does not rely on chicken embryos, has low production cost and short cycle; only one immunization with a low dose can induce strong protective ability. The invention provides a basis for the industrialized production of VLP vaccines.

The invention discloses a traditional Chinese medicine granule for preventing and treating bird flu and improving the immunity of chicken flocks and a preparation method thereof, belonging to the technical fields of animal husbandry and veterinary medicine and traditional Chinese medicine. ~15 parts, Nepeta 8~16 parts, mulberry leaves 10~20 parts, Astragalus 10~25 parts, Guanzhong 3~15 parts, Schisandra chinensis 10~20 parts, honeysuckle 10~25 parts, Echinacea chinensis 15~25 parts, fish 10-18 parts of Houttuynia cordata, 8-15 parts of Clover, and 3-12 parts of Vitex. The traditional Chinese medicine granule provided by the invention has reasonable formula, low cost, convenient drug material acquisition, simple preparation, remarkable preventive and therapeutic effects on bird flu, can improve the immunity of chickens, and has no toxic and side effects.

The invention relates to the technical field of feed production for breeding, and discloses chicken breeding feed for preventing avian influenza. The feed comprises a hollow spherical shell, a strip hole is formed in the shell, the shell is internally provided with a liquid medicine ball, a plurality of through pipes are inserted in the liquid medicine ball in a penetrating mode, one end of each through pipe is located in the liquid medicine ball, a through hole is formed in each through pipe, the other end of each through pipe sequentially penetrates through the liquid medicine ball and the strip hole and extends to the outer side of the shell, each through pipe is sleeved with a sleeve slidingly connected to the interior of the strip hole and connected with a medicine box, each medicinebox includes a straight surface plate and a curved surface plate which are spliced into a hemispherical shape, a medicine cavity communicated with the corresponding through pipe is formed between thecorresponding straight surface plate and the corresponding curved surface plate, the curved surface plates are provided with spraying holes, the straight surface plates are provided with moving parts,the moving parts are connected with plug blocks and fodder balls, the plug blocks can plug the portions communicated with the medicine cavities and the through pipes, the fodder balls are located atthe outer sides of the medicine boxes, and the through pipes are elastic pipes. By means of the chicken breeding feed for preventing the avian influenza, the defect can be overcome that manual medicine mixing is needed for existing feed, and the medicine is prevented from losing efficacy.

The invention discloses a drug for preventing and curing avian influenza. The drug is prepared through the steps as follows: firstly, measuring raw materials: measuring the following raw materials in parts by weight: 71-87 parts of astragali radix, 71-87 parts of taraxacum, 71-87 parts of sculellaria barbata, 29-35 parts of glycyrrhiza, 71-87 parts of grassleaf sweelflag rhizome, 71-87 parts of panax ginseng, 72-87 parts of privet leaves, 72-87 parts of folium isatidis, 71-87 parts of horsetail, 72-88 parts of uncaria, 86-106 parts of lotus roots, 71-87 parts of rosa laevigata, 72-88 parts of mentha; secondly, crushing and uniformly mixing the raw materials, and obtaining the drug for preventing and curing avian influenza. According to the invention, the immunity of the poultry uninfected by avian influenza virus such as chickens and ducks can be improved if the drug is taken in advance, and the drug is good in prophylactic effect and has an excellent curing effect on the poultry with the symptoms of avian influenza.

The invention discloses an algae feed capable of preventing avian influenza and application thereof, discloses an artificially synthesized avian influenza antigen gene optimized by a codon preferred by chlamydomonas reinhardtii, establishes a recombined expression vector suitable for expressing the avian influenza antigen gene in the chlamydomonas reinhardtii, and transfers the recombined expression vector into host chlamydomonas reinhardtii to obtain transgenic chlamydomonas reinhardtii. Animal experiments show that a mouse eating the transgenic chlamydomonas reinhardtii can generate an avian influenza antibody; the algae feed has a wide application prospect and can prevent the avian influenza.