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Method for detecting bird flu and avian infectious bronchitis viruses in livestock farm

A technology for avian influenza virus and bronchitis, applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problem of inaccurate control of epidemic disease control effect, low sensitivity, and ineffective detection of low virus content Samples and other issues, to avoid bird flu infection, improve sensitivity, and reduce economic losses

Inactive Publication Date: 2022-01-04
山东省动物疫病预防与控制中心
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Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a method for detecting avian influenza and avian infectious bronchitis virus in farms, which solves the problem that the traditional pathogen detection method has low sensitivity, cannot effectively detect samples with low virus content, and cannot accurately control The problem of disease control effectiveness

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  • Method for detecting bird flu and avian infectious bronchitis viruses in livestock farm
  • Method for detecting bird flu and avian infectious bronchitis viruses in livestock farm
  • Method for detecting bird flu and avian infectious bronchitis viruses in livestock farm

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Embodiment

[0050] Such as Figure 1-5 As shown, the embodiments of the present invention provide a method for detecting avian influenza and avian infectious bronchitis virus in farms, comprising the following steps:

[0051] S1. Digital PCR primer probe design and synthesis

[0052] Provide two pairs of special primers for virus detection, namely avian influenza virus primer probe set and avian infectious bronchitis virus primer probe set, avian influenza virus primer probe set includes AIV-F, AIV-Probe and AIV-R) , Avian Infectious Bronchitis Virus Primer Probe Set includes IBV-F, IBV-Probe and IBV-R, and the primer sequences are as follows:

[0053] AIV-F:5-TGTACGGACTTGCTGTGGCC-3

[0054] AIV-Probe:(FAM)5-AGTGGATACGACTTTGAGAGAGAAGGGT-3(BHQ1)

[0055] AIV-R:5-GAGACTGAAGACCTGGCTGTT-3

[0056]IBV-F:5-GGTGTAGATCTTGGTGGTCTTT-3

[0057] IBV-Probe:(VIC)5-CCCCGAGAGCAATGCCGTAGGTTC-3(BHQ1)

[0058] IBV-R:5-GTACCATTGGCACACTCTCTAT-3;

[0059] S2. Selection and processing of samples

[0060...

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Abstract

The invention provides a method for detecting bird flu and avian infectious bronchitis viruses in a livestock farm, and relates to the field of biological detection. The method for detecting bird flu and avian infectious bronchitis viruses in the livestock farm comprises the following steps: S1, designing and synthesizing a digital PCR primer probe; S2, selecting and treating a sample; S3, carrying out RT-dPCR process operation; S4, performing a dual RT-dPCR sensitivity experiment; and S5, performing a dual RT-dPCR specificity experiment, wherein the avian influenza virus primer probe group comprises AIV-F, AIV-Probe and AIV-R, and the avian infectious bronchitis virus primer probe group comprises IBV-F, IBV-Probe and IBV-R. By detecting the two pathogens through adoption of the detection method, not only can the detection sensitivity be improved, but also samples with low virus content can be detected, the disease control effect can be accurately controlled, large-scale avian influenza infection is avoided, the economic loss of breeding enterprises is reduced, the detection method can also be used for simultaneously detecting the two pathogens, and therefore, the detection efficiency is obviously improved.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for detecting avian influenza and avian infectious bronchitis virus in farms. Background technique [0002] Avian influenza virus has a wide range of hosts and can cause human infection. Avian infectious bronchitis virus and the current new coronavirus belong to the same virus family. Both viruses have a high infection rate in chicken farms, causing great harm to the breeding industry Economic losses. [0003] The current detection methods for these two pathogens are usually ordinary fluorescence detection, which has low sensitivity and cannot effectively detect samples with low virus content, and cannot accurately control the effect of epidemic control. Contents of the invention [0004] (1) Solved technical problems [0005] Aiming at the deficiencies in the prior art, the present invention provides a method for detecting avian influenza and avian infe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6851C12N15/11
CPCC12Q1/701C12Q1/6851C12Q2600/16C12Q2600/166C12Q2521/107C12Q2563/159C12Q2545/113C12Q2563/107C12Q2537/143
Inventor 薛瑞雪孙圣福陈伟兰邹然王苗利王贵升张月蔺晓月马慧玲徐鸿陈峰李玉杰赵梦娇邢林林
Owner 山东省动物疫病预防与控制中心
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