Preparation method and product of bird flu inactivated vaccine

Abstract

The invention discloses a preparation method of an inactivated bird flu vaccine and its product. The preparation method comprises: preparing a bird flu virus liquid; adding formaldehyde to the bird flu virus liquid to inactivate the virus liquid; adding an adjuvant to the inactivated virus liquid , emulsified, and obtained; wherein, before adding the adjuvant to the inactivated virus liquid, the virus liquid is subjected to the following treatment: the inactivated virus liquid is concentrated, and the concentrated virus liquid is diluted. The present invention aims at the production status of avian influenza inactivated vaccines, optimizes and improves the production process of avian influenza vaccines, does a large number of experiments on the relationship between formaldehyde concentration and immune titer, and uses virus Concentration technology effectively removes formaldehyde residues in vaccine products. Compared with existing avian influenza inactivated vaccines, the method of the present invention effectively removes 75% of formaldehyde residues in vaccine products, and the prepared avian influenza inactivated vaccines have immunity Small side effects, long storage period, high antibody level and other advantages.

Description

technical field [0001] The invention relates to a preparation method of an inactivated vaccine, in particular to a preparation method of an avian influenza inactivated vaccine and an inactivated vaccine product prepared by the method, and belongs to the field of production of an avian influenza inactivated vaccine. Background technique [0002] Avian influenza is the abbreviation of avian influenza, which is an acute hemorrhagic infectious disease caused by a subtype of influenza A virus (also known as avian influenza virus) to cause respiratory disease or latent infection as a feature. The World Organization for Animal Health (OIE), the International Trade Organization and the International Veterinary Bureau have designated highly pathogenic avian influenza HPAI as a class A infectious disease, also known as "true chicken plague" or "European chicken plague". [0003] Avian influenza virus infection can manifest as mild respiratory symptoms and gastrointestinal symptoms, a...

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the defects such as poor safety, short storage period and poor protection effect of the existing avian influenza inactivated vaccine containing high concentration of formaldehyde residue, and provide a new avian influenza inactivated vaccine. A preparation method for a live vaccine. The preparation method can effectively remove formaldehyde residues in the inactivated avian influenza vaccine. The prepared inactivated avian influenza vaccine has the advantages of small immune side effects, long shelf life, and high antibody level.

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