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Simulated tissue culture method and application

A simulated group and seedbed technology, applied in the field of seedlings, can solve the problems of high cost, long seedling cycle, low seedling survival rate, etc., and achieve the effects of fast growth, short seedling cycle and fast rooting

Inactive Publication Date: 2017-06-06
康盛京构(北京)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved in the present invention is to overcome the low seedling survival rate existing in the existing seedling raising method, the long seedling raising cycle and the high defects of cost, thereby providing a kind of simulation group cultivation with high survival rate, short seedling raising cycle and low cost Seedling method and application

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  • Simulated tissue culture method and application
  • Simulated tissue culture method and application
  • Simulated tissue culture method and application

Examples

Experimental program
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Embodiment 1

[0040] This embodiment provides a method for cultivating seedlings in a simulated group. In April, 1200 hybrid Broussonetia papyrifera seedlings (1 year old, 150cm in height) from Dongguan Science and Technology Park in Huairou District, Beijing, with fixed stems of 60cm, were used as female parent tree seedlings. To collect seedling materials, the simulation group of Broussonetia papyrifera was cultivated in Dongguan Science and Technology Park, Huairou District, Beijing. The steps are as follows:

[0041] (1) Construct the arch sheds, build 26 of the arch sheds, each of the arch sheds has an arch width of 2.1m, an arch height of 1.2m, the total length of the arch sheds is 24m, and a seedling bed for growing seedlings is set in the arch sheds The matrix of the seedbed is prepared according to the mass ratio of peat: perlite: vermiculite of 3:1:1, and the matrix and water are mixed and uniformly loaded into the 72-hole plug tray according to the mass ratio of 8:1. Each of the plu...

Embodiment 2

[0047] This embodiment provides a method for cultivating seedlings in a simulated group. In April, 50,000 hybrid Broussonetia papyrifera seedlings (2-year-old, 160cm height) planted in Menghe Base, Changzhou City, Jiangsu Province, with a fixed stem of 72cm, were used as the female parent tree. To collect seedling materials, a simulation group of Broussonetia papyrifera was cultivated in Menghe Base, Changzhou City, Jiangsu Province. The steps are as follows:

[0048] (1) Construct the arch shed, build 26 the arch shed, the arch width of each arch shed is 2.2m, the arch height is 1m, the total length of the arch shed is 24m, and a large arch shed is built outside the arch shed. In order to shelter rainwater, a seedling bed for raising seedlings is arranged in the arch shed. The seedbed substrate is prepared according to the mass ratio of peat: perlite: vermiculite of 4:1:1, and the mass ratio of the substrate to water is 10: 1 Mix and evenly put into the 72-hole plug tray, a numb...

Embodiment 3

[0054] This embodiment provides a method for cultivating seedlings in a simulated group. In April, 4000 tomato seedlings (two-year-old) planted in Menghe Base, Changzhou City, Jiangsu Province, were used as female parent trees to collect seedling materials. The Menghe Base in Changzhou City, the province carried out the simulation group cultivation of seedlings of the tomato tree, the steps are as follows:

[0055] (1) Construct the arch shed, build 26 the arch shed, the arch width of each arch shed is 2.2m, the arch height is 1m, the total length of the arch shed is 24m, and a large arch shed is built outside the arch shed. In order to shelter rainwater, a seedling bed is set in the arch shed, and the seedbed substrate is prepared according to the mass ratio of peat: perlite: vermiculite of 2:1:1, and the mass ratio of the substrate to water is 9: 1 Mix and evenly put into the 72-hole plug tray, a number of the plug trays are tightly discharged to form the seedbed, the top of th...

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Abstract

The invention discloses a simulated tissue culture method and an application. The simulated tissue culture method includes the steps: (1) building an arched shed, arranging a seedling bed for seedling culture in the arched shed, sealing the arched shed and sterilizing and disinfecting the inside of the arched shed; (2) collecting explants for simulating tissue culture, performing pretreatment, inserting the explants into the seedling bed and sealing the arched shed; (3) sterilizing and disinfecting the inside of the arched shed, controlling the internal temperature, the relative humidity and the illumination intensity of the arched shed and maintaining seedling culture; (4) hardening seedlings; (5) transplanting the seedlings to a land for growing field crops. According to the method, cuttage branches can be kept in a relatively aseptic environment for seedling culture before rooting, the branches are not easily polluted or corroded by mixed bacteria, the seedling culture environment is excellent, rooting and growth are rapid, survival rate is high, investment cost is low, materials are easily obtained, seedling culture number is large, three batches of seedlings can be cultured in one arched shed within one year, and seedling culture efficiency and economic benefits are high.

Description

Technical field [0001] The invention belongs to the technical field of seedling raising, and specifically relates to a method for raising seedlings by using simulated tissue culture technology and its application. Background technique [0002] At present, the commonly used techniques for raising seedlings include cutting seedlings and group breeding of seedlings. Among them, cutting seedlings technology is due to its slow reproduction speed (one generation per year), low reproduction rate, and poor survival rate (generally only 30-60%). Only some plants that are easy to take root can be propagated and seedlings routinely, but for new varieties and rare tree species, they cannot achieve the purpose of rapid propagation and marketing in a short period of time, which is far from meeting people's needs; while tissue culture technology can compensate for cuttings. The defects of seedling breeding have the advantages of fast reproduction and high survival rate. However, because tissue ...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G9/10A01G13/02A01G13/00
CPCA01G31/00A01G9/0299A01G13/00A01G13/0231A01G24/00
Inventor 王福喜
Owner 康盛京构(北京)科技有限公司
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