Technological method for producing gentamicin C1a through fermentation

Abstract

The invention relates to a technological method for producing gentamicin C1a through fermentation, and belongs to the technical field of fermentation engineering. The technological method for producing gentamicin C1a through fermentation comprises the following concentrated technological means: spore culture, seed liquid preparation, primary seed culture, secondary seed culture, fermentation production and material supplementation control, wherein components and proportions of a primary seed culture medium, a secondary seed culture medium and a fermentation culture medium are mainly provided by the technological method; meanwhile, in the material supplementation control stage, sugar supplementing, water supplementing, defoamer supplementing, alkali supplementing and nitrogen supplementing operations are performed, and the material supplementing time, the material supplementing components and the material supplementing amount are controlled. The technological method is achieved by relying on culture media for producing the gentamicin C1a through efficient and low-cost fermentation, and in the fermentation process, technological control is performed, so that the fermentation titer unit is improved and the yield is increased by 43%; meanwhile, the production cost is reduced to the maximum extent; therefore, the technological method is very practical during production of the gentamicin C1a.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, in particular relates to the fermentation production of gentamicin, in particular to a process for producing gentamicin C1a by fermentation. Background technique [0002] Gentamicin C1a is the mother nucleus precursor of a semi-synthetic new drug ictimicin, which is the first in China and has independent intellectual property rights. The traditional method of preparing C1a is to use resin separation technology to extract C1a single component from multi-component gentamycin. Due to the similar structure, similar physical and chemical properties and equivalent yield of each component, the extraction cost remains high. , the price of high-purity C1a remains high on the market. [0003] In the existing literature, it has been reported from the scientific research point of view to use fermentation shake flasks to incubate the seed liquid and then carry out gentamicin C1a fermentation...

AI Technical Summary

Problems solved by technology

The traditional method of preparing C1a is to use resin separation technology to extract C1a single component from multi-component gentamycin. Due to the similar structure, similar physical and chemical properties and equivalent yield of each component, the extraction cost remains high. , the price of high-purity C1a remains high in the market

[0003] In the existing literature, it has been reported from the scientific research point of view to use fermentation shake flasks to incubate the seed liquid and then carry out gentamicin C1a fermentation and culture, but the industrial production has not yet been realized, and the practical application and determination of various parameters in the production process are not very clear. clear

At present, the culture medium and technology for fermenting gentamicin C1a are basically based on the technology of gentamicin fermentation and production, but there are problems such as low micromonospora micromonospora production rate, many fermentation materials and long cycle in the production process, resulting in The production cost is generally high. In addition, although the gentamicin C1a strain itself and the gentamicin production strain belong to the Micromonospora genus, there are large differences in physiological and metabolic characteristics, and the gentamicin C1a strain is exclusively used for the production of gentamicin C1a. Fermentation media and culture methods are not perfect

Images