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Efficient tissue culture method of Cattleya hybrida

A technology of tissue culture and culture method, applied in the field of high-efficiency tissue culture of Cattleya, can solve the problems of restricting the development of the industry, restricting the factory breeding and reproduction of Cattleya, and improving the efficiency of tissue culture and optimizing the induction of callus. and the effect of the proliferation system

Inactive Publication Date: 2017-06-09
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Realizing the rapid propagation of Cattleya through tissue culture is not only conducive to maintaining and multiplying some precious Cattleya resources, but also conducive to its industrial development. Difficulties in the process restrict Cattleya factory breeding and reproduction, limiting the development of the industry

Method used

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specific Embodiment approach

[0013] The culture method of Cattleya high-efficiency tissue culture of the present invention, its preferred embodiment is:

[0014] Include steps:

[0015] Explant disinfection: use 10% NaClO, 5% NaClO, 2% NaClO, 1% NaClO, 0.5% NaClO for disinfection;

[0016] Prevention of browning and primary culture: 1 / 2MS + sucrose 20g / L + agar 0g / L + PVP 6g / L, 10 days of dark culture treatment, culture temperature 18 to 22 ℃;

[0017] Callus induction and subculture: MS + sucrose 20g / L + agar 5.8g / L + 6-BA3mg / L + NAA0.5mg / L + PVP3g / L. =5.7, the culture temperature is 23 to 27°C, and the light intensity is 60μmol·m -2 the s -1 , light time 12 hours / day.

[0018] Rooting and strong seedlings: MS+NAA0.1mg / L+6-BA2.0mg / L+banana 10g / L+mashed potatoes 100g / L+sucrose 30g / L+activated carbon 1g / L+agar 4g / L, pH=5.7, culture temperature 23 to 27 ℃, the light intensity is 60μmol·m -2 the s -1 , light time 12 hours / day.

[0019] The cultivation method of efficient tissue culture of Cattley...

specific Embodiment

[0021] (1) Disinfection of explants: the new shoots selected from healthy plants are explants, and the buds are about 6cm long. After the buds are cut off from the mother plant, they are rinsed with flowing tap water for 30 minutes in the laboratory, and stripped in an ultra-clean bench. Dip the outermost layer of bracts in 70% alcohol (1-2s), immediately soak in 10% NaClO aqueous solution for 10 minutes, shake slightly, rinse with sterile water immediately after taking it out, and peel off a layer Put the bracts into 5% NaClO aqueous solution for 5 minutes and rinse them with sterile water. By analogy, the disinfection time is gradually shortened until the last buds are left. Take the growth point as the center, cut out the stem tip with a diameter of about 5 mm on the petri dish, soak it in 0.5% NaClO aqueous solution, take it out after 1 min, rinse it with sterile water several times, and place it on the prepared medium.

[0022] (2) The explants were inoculated into medium...

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Abstract

The invention discloses an efficient tissue culture method of Cattleya hybrida, comprising: sterilizing explants; preventing browning, and performing primary culture, callus induction and subculture; rooting and strengthening, to be specific, culturing with 0.1 mg / L of MS+NAA, 2.0 mg / L of 6-BA, 10 g / L of banana, 100 g / L of mashed potato, 30 g / L of sucrose, 1 g / L of activated carbon and 4 g / L of agar under pH of 5.7 at the temperature of about 23-27 DEG C, and exposing to light under the intensity of 60 Mumol.m<-2>s<-1> for 12 hours per day. The Cattleya hybrida callus induction and proliferation system is optimized, and factory tissue culture efficiency of Cattleya hybrida is improved.

Description

technical field [0001] The invention relates to a flower seedling raising and propagation technology, in particular to a method for cultivating Cattleya efficient tissue culture. Background technique [0002] Cattleya belongs to the genus Cattleya of the Orchidaceae family. It is native to Central and South America, especially Colombia and Brazil. It is also known as the world's four largest ornamental orchids together with Dendrobium, Phalaenopsis, and Oncidium. Cattleya has different varieties blooming throughout the year. Because of its large and beautiful flowers, rich and colorful colors and most of them are fragrant, it is recognized as the "King of Cattleya" in the world. It is not only an excellent indoor potted flower, but also an important high-end plant. cut flowers. [0003] In recent years, with the rapid development of my country's flower industry and the improvement of people's living standards, Cattleya production has also risen rapidly, and has occupied a c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 郑宝强王雁郝婕
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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