Unlock instant, AI-driven research and patent intelligence for your innovation.
Phenolphthalein hapten, artificial antigen and preparation method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of artificial antigen and hapten, applied in the field of artificial antigen and its preparation, phenolphthalein hapten, can solve the problems of high cost and long time.
Active Publication Date: 2019-07-09
GUANGDONG UNIV OF TECH
View PDF2 Cites 1 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
At present, traditional methods are used to detect whether phenolphthalein is contained in Chinese patent medicines or health care products, and the extracted samples must be brought back to the laboratory for large-scale instrumental analysis, which takes a long time and costs high
However, there is no relevant report about phenolphthalein monoclonalantibody
The design of high-potency phenolphthalein antigens is still difficult for skilled workers
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
preparation example Construction
[0062] In the embodiment provided by the present invention, the preparation method of the hapten shown in formula I comprises steps:
[0063] 1) Mix 1 part of phenolphthalein with 4-10 parts of aliphatic diamine, stir and react at 130°C for 12 hours, and concentrate the product to near dryness under reduced pressure to obtain an oily crude product; add 50 parts of pure water, ultrasonically disperse and wash, and collect the solid by suction filtration , and dried to obtain aminated phenolphthalein derivatives.
[0064] 2) Mix 1 part of aminated phenolphthalein derivative with 2 to 5 parts of cyclic anhydride, dissolve in 30 parts of DMF, add 5 drops of triethylamine, the pH value is about 7.5, and react under closed stirring at 40-50°C 10-12h. After the reaction, the reaction solution was poured into water, extracted 3 times with 100 parts of ethyl acetate, the combined extracts were washed with anhydrous Na 2 SO 4 After drying and concentrating, a solid was obtained, whic...
Embodiment 1
[0083] The preparation method of embodiment 1 aminated phenolphthalein (Scheme 1: n=1)
[0084] Mix 1 part of phenolphthalein with 8 parts of ethylenediamine, stir and react at 130°C for 12 hours, and concentrate the product to near dryness under reduced pressure to obtain an oily crude product; add 50 parts of pure water, ultrasonically disperse and wash, collect the solid by suction filtration, and dry to obtain aminated Phenolphthalein derivatives.
[0085] The structure of the resulting product is characterized by: 1 H NMR (400MHz, DMSO) δ7.86(d, J=8.0Hz, 1H), 7.52(t, J=7.8Hz, 1H), 7.16(t, J=8.6Hz, 1H), 7.10–7.03(m , J=8.0Hz, 5H), 6.75(d, J=8.2Hz, 4H), 3.40(t, J=8.3Hz, 2H), 2.85(t, J=6.9Hz, 2H).
Embodiment 2
[0086] The preparation method of embodiment 2 aminated phenolphthalein (Scheme 1: n=2)
[0087] 1 part of phenolphthalein was mixed with 8 parts of propylenediamine, and the subsequent steps were the same as in Example 1.
[0088] The structure of the resulting product is characterized by: 1 H NMR (400MHz, DMSO) δ7.79(d, J=7.4Hz, 1H), 7.58(t, J=8.5Hz, 1H), 7.20–7.13(m, J=7.5Hz, 5H), 7.01(d ,J=8.5Hz,1H),6.68(d,J=8.8Hz,4H),3.15(t,J=7.8Hz,2H),2.60(t,J=6.4Hz,2H),1.61–1.55(m ,2H).
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention relates to the biotechnology field, in particular to phenolphthaleinhapten, a phenolphthaleinartificial antigen and preparation methods of the phenolphthaleinhapten and the phenolphthalein artificial antigen. According to the hapten, a linker arm structure and an active group used for coupling with macromolecules are introduced on the basis that the basic structure of phenolphthalein is retained, so that coupling of the hapten and the macromolecules is facilitated, the molecular structure of hapten and the basic structure of phenolphthalein with relatively small molecular weight are sufficiently exposed after coupling, and the situation that the hapten is shielded by macromolecules and cannot be recognized by animal bodies is avoided. The artificial antigen is prepared by coupling the hapten and protein with an active esterification method, the coupling ratio can reach 18.2, and the phenolphthalein hapten and the phenolphthalein artificial antigen can be used for immunodetection of phenolphthalein.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a phenolphthalein hapten, an artificial antigen and a preparation method thereof. Background technique [0002] Phenolphthalein, 3,3-bis(4-hydroxyphenyl)-1(3H)-isobenzofuranone, can act on intestinal smooth muscle, increase intestinal peristalsis, and at the same time inhibit the absorption of water in the intestine, Accumulate water and electrolytes in the colon and produce a laxative effect. It is often used clinically to treat habitual intractable constipation. Its action is mild and rarely causes intestinal spasms. However, phenolphthalein has certain adverse reactions, including: allergic reactions caused by phenolphthalein are clinically rare, and can occasionally cause dermatitis, drug eruption, itching, burning pain, enteritis, and bleeding tendency. Contraindications include appendicitis, undiagnosed rectal bleeding, congestive heart failure, hypertension, fecal blockage, ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More