A method to improve the regeneration efficiency of apple transgenic materials
A regeneration efficiency and transgenic technology, applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of low efficiency of leaf regeneration plants, single transgenic materials, etc., and achieve low transformation efficiency, strong stress resistance, The effect of improving efficiency
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[0018] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0019] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.
[0020] Such as figure 1 As shown, the method for improving the regeneration efficiency of apple transgenic materials provided by the embodiments of the present invention comprises the following steps:
[0021] S101: culturing the tissue-cultured seedlings of Begonia rotifera in the propagation medium for one month, cutting off the callus at the base of the stem with a scalpel, and then transplanting the regenerated stem segment into a new medium;
[0022] S102: The regenerated stem s...
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